Composite microbial feed additive and preparation method thereof

A feed additive and compound microorganism technology, applied in the field of feed additives, can solve the problems of single regulation, poor controllability, and decreased stability of probiotics, and achieve the effects of enhancing digestion and absorption, good stability, and promoting animal growth and development.

Inactive Publication Date: 2018-12-18
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a single type of probiotic has a relatively simple regulatory effect on the body, and the combination of multiple probiotics has disadvantages such as decreased stability and poor controllability during the compounding process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A compound microbial feed additive, which is formed by mixing lactic acid bacteria fermentation products, bifidobacteria fermentation products and Bacillus subtilis fermentation products according to the mass ratio of raw materials at 1:1:1, and the effective live bacteria amount is 1.2×10 9 CFU / ml.

[0024] Preparation:

[0025] 1) Preparation of Lactobacillus fermentation products and Bifidobacteria fermentation products: Take Lactobacillus and Bifidobacteria and place them in MRS solid medium respectively, separate and purify them at pH 6.3 and culture temperature 37°C for 20 hours; then transfer to liquid culture medium at pH 6.7 and culture temperature 30°C for 24 hours; then transferred to fermentation medium and fermented at pH 6.7 and culture temperature 30°C for 24 hours to obtain Lactobacillus fermentation products and bifidobacteria Bacillus fermentation products;

[0026] 2) Preparation of Bacillus subtilis fermentation product: take Bacillus subtilis and ...

Embodiment 2

[0031] A compound microbial feed additive, which is formed by mixing lactic acid bacteria fermentation products, bifidobacteria fermentation products and Bacillus subtilis fermentation products according to the mass ratio of raw materials at 1:1:1, and its effective live bacteria amount is 1.3×10 9 CFU / ml.

[0032] Preparation:

[0033] 1) Preparation of Lactobacillus fermentation products and Bifidobacteria fermentation products: Take Lactobacillus and Bifidobacteria and place them in MRS solid medium respectively, separate and purify them at pH 6.7 and culture temperature 30°C for 24 hours; then transfer to liquid culture medium at pH 6.3 and culture temperature 37°C for 20 hours; then transferred to fermentation medium and fermented at pH 6.7 and culture temperature 30°C for 24 hours to obtain Lactobacillus fermentation products and bifidobacteria Bacillus fermentation products;

[0034] 2) Preparation of Bacillus subtilis fermentation product: take Bacillus subtilis and ...

Embodiment 3

[0039] A compound microbial feed additive, which is formed by mixing lactic acid bacteria fermentation products, bifidobacteria fermentation products and Bacillus subtilis fermentation products according to the mass ratio of raw materials at 1:1:1, and the effective live bacteria amount is 1.37×10 9 CFU / ml.

[0040] Preparation:

[0041] 1) Preparation of Lactobacillus fermentation products and Bifidobacteria fermentation products: Take Lactobacillus and Bifidobacteria and place them in MRS solid medium respectively, separate and purify them at pH 6.5 and culture temperature 35°C for 22 hours; then transfer to liquid culture medium for 22 hours at a pH value of 6.5 and a culture temperature of 35°C; then transferred to a fermentation medium and fermented for 22 hours at a pH value of 6.5 and a culture temperature of 35°C to obtain Lactobacillus fermentation products and bifidobacteria Bacillus fermentation products;

[0042] 2) Preparation of Bacillus subtilis fermentation p...

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PUM

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Abstract

The present invention discloses a composite microbial feed additive. The composite microbial feed additive is prepared by mixing a lactic acid bacterium fermentation product, a bifidobacterium fermentation product and a bacillus subtilis fermentation product according to a raw material mass ratio of 1:1:1. Composite microorganisms have good stability, can synthesize various nutrients in animal intestinal reproductive processes, enhance digestion and absorption of nutrients, promote growth and development of animals, ensure structural integrity of intestinal tract states by improving height ofintestinal villi, reducing depth of crypts and increasing muscle thickness, maintain the optimal immune states of the intestinal tracts, at the same time can promote high expression of an immune geneMHC I and improve immune performance of body. The present invention also discloses a preparation method of the composite microbial feed additive. The preparation method is simple to operate, strong incontrollability and favorable for large-scale industrial production.

Description

technical field [0001] The invention relates to the field of feed, in particular to a feed additive. Background technique [0002] At present, auxin and antibiotic feed additives are widely used in the aquaculture industry. Although the use of these feed additives can promote aquaculture to a certain extent, it will cause a large amount of drug residues and increase bacterial resistance. directly or indirectly cause harm to human health through continuous accumulation in animals. At present, social food safety issues have attracted much attention, and people pay more attention to green and healthy aquatic products. At present, many countries in the world have issued laws to prohibit the addition of auxin and antibiotic feed additives in feed, making the use of such additives more efficient. strict. [0003] Probiotics are a class of active microorganisms that are beneficial to the host, and can produce definite health effects, thereby improving the host's micro-ecological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/30A23K10/37A23K20/24
CPCA23K10/12A23K10/30A23K10/37A23K20/24A23V2400/11A23V2400/51Y02P60/87
Inventor 刘华杨映谢明宝岑嘉成彭钟琴于辉
Owner FOSHAN UNIVERSITY
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