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Method for extracting flavonoid compounds from persimmon leaves

A technology of flavonoids and persimmon leaves, which is applied in the field of extraction of flavonoids, can solve the problems of low content of flavonoids, use of many solvents, and long process time, and achieve simple and easy operation methods, increase extraction content, and eliminate delays. The effect of the tail phenomenon

Pending Publication Date: 2018-12-21
西安利君精华药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used extraction method is solvent extraction. Solvent extraction has problems such as long time-consuming process, many solvents used, high cost of solvent and solvent recovery, low yield and many impurities, and the content of extracted flavonoids is low. Some extraction processes are improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The invention provides the following technical scheme: a method for extracting flavonoids from persimmon leaves, comprising the following steps:

[0026] A. Pick fresh persimmon leaves, rinse and dry: use clean water to rinse 3 times repeatedly, and dry in a ventilated place;

[0027] B, the dried persimmon leaves are added into a pulverizer for pulverization, and then passed through a 30-mesh sieve to obtain the pulverized persimmon leaves;

[0028] C, the pulverized persimmon leaves after sieving were added into ethanol, acetic acid, formic acid mixed aqueous solution and carried out ultrasonic extraction 2 times to obtain an extract, wherein the ultrasonic power was 500W, and the ultrasonic time was 5min;

[0029] D, add sodium hydroxide aqueous solution to carry out microwave extraction 2 times in primary extraction liquid, obtain secondary extract;

[0030] E, the secondary extract is subjected to ultrafiltration, followed by vacuum concentration to obtain a conce...

Embodiment 2

[0043] A method for extracting flavonoids from persimmon leaves, comprising the following steps:

[0044] A. Pick fresh persimmon leaves, rinse and dry: use clear water to rinse 5 times repeatedly, and dry in a ventilated place;

[0045] B, the dried persimmon leaves are added into a pulverizer for pulverization, and then passed through a 30-mesh sieve to obtain the pulverized persimmon leaves;

[0046] C, adding the sieved persimmon leaves pulverized matter into ethanol, acetic acid, formic acid mixed aqueous solution and carrying out ultrasonic extraction 2 times to obtain an extract, wherein the ultrasonic power is 800W, and the ultrasonic time is 10min;

[0047] D, add sodium hydroxide aqueous solution to carry out microwave extraction 2 times in primary extraction liquid, obtain secondary extract;

[0048] E, the secondary extract is subjected to ultrafiltration, followed by vacuum concentration to obtain a concentrate; wherein, the vacuum concentration method is as foll...

Embodiment 3

[0061] A method for extracting flavonoids from persimmon leaves, comprising the following steps:

[0062] A. Pick fresh persimmon leaves, rinse and dry: use clean water to rinse 3 times repeatedly, and dry in a ventilated place;

[0063] B, the dried persimmon leaves are added into a pulverizer for pulverization, and then passed through a 30-mesh sieve to obtain the pulverized persimmon leaves;

[0064]C, the pulverized persimmon leaves after sieving were added into ethanol, acetic acid, formic acid mixed aqueous solution and carried out ultrasonic extraction for 2 times to obtain an extract, wherein the ultrasonic power was 550W, and the ultrasonic time was 6min;

[0065] D, add sodium hydroxide aqueous solution to carry out microwave extraction 2 times in primary extraction liquid, obtain secondary extract;

[0066] E, the secondary extract is subjected to ultrafiltration, followed by vacuum concentration to obtain a concentrate; wherein, the vacuum concentration method is ...

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Abstract

The invention discloses a method for extracting flavonoid compounds from persimmon leaves. The method comprises the following steps: A, picking the fresh persimmon leaves, rinsing and airing; B, crushing the aired persimmon leaves, and sieving; C, adding the sieved crushed persimmon leaf material into a mixed aqueous solution of ethanol, acetic acid and formic acid, and performing ultrasonic extraction twice to obtain a primary extract; D, adding an aqueous sodium hydroxide solution into the primary extract, and performing microwave extraction twice to obtain a secondary extract; E, performingultrafiltration on the secondary extract, and then performing vacuum concentration to obtain a concentrate; F, drying the concentrate at low temperature to obtain the flavonoid compounds. The operation method adopted in the invention is simple, easy to implement and low in cost; according to the method, the content of the extracted flavonoid compounds can be increased.

Description

technical field [0001] The invention relates to the technical field of extracting flavonoids, in particular to a method for extracting flavonoids from persimmon leaves. Background technique [0002] Persimmon leaves are the leaves of Persimmon persimmon, which has been used as a natural medicine for a long time. Persimmon leaves are rich in flavonoids. Most of these flavonoids exist in the form of quercetin, kaempferol and their glycosides. Studies have shown that these substances have anti-oxidation, anti-allergic, antibacterial, blood circulation and hemostasis, enhance immunity, and protect the heart. The total flavonoids extracted from persimmon leaves have great market value in brewing vinegar. The main active ingredients in persimmon leaves are flavonoids, and the total content of flavonoids in persimmon leaves is 121.3mg / 100g. The flavonoids that have been found so far include astragaloside (molecular weight: 446.36), isoquercetin (338.27), Naphthol 3-o-β-D-glucosid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/44A61P39/06A61P37/08A61P31/04A61P7/04A61P7/06A61P9/00A61P37/02
CPCA61K36/44A61K2236/13A61K2236/15A61K2236/30A61K2236/33A61K2236/333A61K2236/51A61P7/04A61P7/06A61P9/00A61P31/04A61P37/02A61P37/08A61P39/06
Inventor 王锋怀
Owner 西安利君精华药业有限责任公司
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