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Method for preparing conditioner accelerating seed germination

A technology for regulators and seeds, applied in the direction of plant growth regulators, plant growth regulators, botany equipment and methods, etc., to achieve the effects of optimized synthesis, convenient operation, and promotion of seed germination

Inactive Publication Date: 2019-09-10
海盐县凌特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few research reports on the use of coumarin compounds to promote plant seed germination, which has certain development and application prospects

Method used

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  • Method for preparing conditioner accelerating seed germination
  • Method for preparing conditioner accelerating seed germination
  • Method for preparing conditioner accelerating seed germination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Regulators for accelerating seed germination, including 50% copper algae extract, 1% coumarin compounds and 49% plant ash solution;

[0051] The preparation method of the regulator of accelerating seed germination:

[0052] Wherein, the preparation method of copper algae extract is: fresh copper algae air sacs are cleaned and crushed, add extraction solvent, the volume ratio of copper algae air sacs and extraction solvent is 1:2.5g / mL, at 30 ℃, apply magnetic field ( The magnetic induction intensity is 0.05T) and extracted for 1.5 hours, then concentrated in vacuum at 25°C, left to crystallize, and finally concentrated in the water phase to obtain the copper algae extract; the extraction solvent is methanol-water-vanillic acid-glutathione in a volume ratio of 15 :1:0.5:0.2;

[0053] Coumarins are of the following chemical formula,

[0054]

[0055] Among them, R 1 and R 2 Both are hydroxyl groups; the use concentration of the coumarin compound is 10 -7 mol / L.

...

Embodiment 2

[0060] Regulators to accelerate seed germination, including 56% copper algae extract, 5% coumarin compounds and 39% plant ash solution;

[0061] The preparation method of the regulator of accelerating seed germination:

[0062] Wherein, the preparation method of copper algae extract is: fresh copper algae air sacs are cleaned and crushed, add extraction solvent, the volume ratio of copper algae air sacs and extraction solvent is 1:5g / mL, at 50 ℃, apply magnetic field (magnetic induction The strength is 0.6T) and extracted for 1 hour, then concentrated in vacuum at 30°C, left to crystallize, and finally concentrated in the water phase to obtain the copper algae extract; the extraction solvent is methanol-water-vanillic acid-glutathione in a volume ratio of 20: 1:0.5:0.1;

[0063] Coumarins are of the following chemical formula,

[0064]

[0065] Among them, R 1 is methoxy, R 2 is a carboxyl group; the use concentration of this coumarin compound is 10 -6 mol / L.

[0066]...

Embodiment 3

[0072] Investigate the present invention to accelerate the impact of the regulator of seed germination on the apple seed germination rate, the results are as table 3; buy apple seeds totally 60, every group of 20 seeds, the seeds are soaked with respective regulators respectively, put into a germination bag . Continue to add regulators of respective concentrations in the bag, and use clear water as a negative control group, and commercially purchased common liquid fertilizer as a positive control group.

[0073] Table 3 The present invention accelerates the regulator of seed germination on the influence of apple seed germination rate

[0074]

[0075] As can be seen from Table 3, the regulator for accelerating seed germination of the present invention can significantly promote seed germination; the promotion mechanism is to regulate the content and ratio of gibberellin and abscisic acid in the plant by affecting the activity of relevant enzymes in the immature embryos of pl...

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Abstract

The invention provides a method for preparing a conditioner accelerating seed germination. The method includes preparation of a Sargassum horneri extract. The method includes steps of cleaning fresh Sargassum horneri air sacs, stirring the air sacs to crush the air sacs, adding an extracting solvent, performing extraction at 30-60 DEG C under the function of an external magnetic field, performingvacuum concentration at 25-32 DEG C, performing standing for crystallization and performing water-phase concentration to obtain the Sargassum horneri extract, with the extracting solvent being methanol-water-vanillic acid-glutathione in a volume ratio of 15-25:1:0.5:0.1-0.2. The conditioner comprises 50-60% of the Sargassum horneri extract and 1-8% of a coumarin compound as effective components. The conditioner can regulate and control contents and the ratio of gibberellins and abscisic acid in plants through affecting activity of related enzymes in plant seed immature embryos, thus promotingseed germination.

Description

technical field [0001] The invention belongs to the field of promoting plant growth, and in particular relates to a preparation method of a regulator for accelerating seed germination. Background technique [0002] Seaweeds are plants that live in the ocean and come in many shapes and sizes. Among them, macroalgae are mainly red algae, green algae and brown algae according to their different colors. For example, red algae include seaweed, agarose, etc.; green algae include enteromorpha, Ulva, etc.; brown algae include kelp, hijiki, etc. There are many nutrients in seaweed, including protein, fat, sugar, and various plant hormones, minerals, and trace elements. They are not only the nutrients that people like, but also the nutrients that plants need for growth, which can make plants grow longer. High, promote germination, the fruit is bigger and sweeter, and it is beneficial to the maintenance of the soil, and is more beneficial to human health. my country's research and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/03A01N43/16A01P21/00A01C1/00
CPCA01C1/00A01N43/16A01N65/00A01N65/03
Inventor 董珊珊
Owner 海盐县凌特生物科技有限公司
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