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Extraction method and application of tobacco anti-tumor active components

A technology of anti-tumor activity and extraction method, which is applied in the field of extraction of tobacco anti-tumor active components, can solve the problems of toxicity, flammability, and volatility, etc., achieve broad application prospects, simple preparation method, and increase the effect of extraction content

Inactive Publication Date: 2019-06-21
GUIZHOU TOBACCO SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, low-polarity solvents such as petroleum ether, n-hexane, medium-polarity solvents acetone, and methylene chloride are mostly volatile, flammable, and toxic solvents, which have high requirements for industrial production equipment and personnel, and the obtained tobacco activity The content of components is small, which greatly limits the further research and application of tobacco active components

Method used

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  • Extraction method and application of tobacco anti-tumor active components
  • Extraction method and application of tobacco anti-tumor active components
  • Extraction method and application of tobacco anti-tumor active components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Extraction of Tobacco Antitumor Active Components

[0039] 1. The extraction steps of tobacco anti-tumor active components are as follows:

[0040] (1) Pretreatment.

[0041] Weigh 30Kg of tobacco raw material, place it in an oven for drying, the drying temperature is 60°C, the drying time is 4h, and take out until the moisture content of the tobacco is less than 5%; take the dried tobacco raw material, and use a 50-mesh screen After being pulverized by a pulverizer, it is put into a superfine pulverizer for superfine pulverization treatment to obtain tobacco superfine powder.

[0042] (2) extraction with normal temperature water.

[0043]Weigh 30Kg of tobacco superfine powder into a 200L extraction tank, add 100Kg of purified water, stir and extract at room temperature for 0.5h; of sediment.

[0044] (3) High temperature water extraction.

[0045] Weigh 25Kg of the precipitate in step (2) and add it to a 100L concentrated extraction tank; add 60Kg of pu...

Embodiment 2

[0054] Embodiment 2: the experiment that tobacco active component suppresses the growth of human tumor A549 cell

[0055] Blank control wells: no drug was added, and DMSO with a final concentration of 1% was added;

[0056] Experimental hole: add the antitumor active component that embodiment 1 step (6) (b) gains

[0057] Cell culture:

[0058] at 5% CO 2 , 37° C., under saturated humidity, cultured human lung tumor A549 cells in RPMI 1640 (10% FBS+1% PS) medium, and selected the cells growing in the logarithmic phase as experimental cells. After counting the cells, dilute it with medium to obtain a cell suspension of about 58,000 per milliliter.

[0059] Cell growth monitoring:

[0060] Place the cell real-time monitor in 5% CO 2 , 37 ℃ saturated humidity incubator. Take an 8-well plate, add 150 μL of RPMI 1640 medium to each well, put it into the real-time cell monitor for baseline, take out the eight-well plate after the baseline, and add 345 μL of diluted A549 cell s...

Embodiment 3

[0061] Embodiment 3: the test of suppressing the growth of human liver cancer Hep-G2 cells

[0062] Blank control wells: no drug was added, and DMSO with a final concentration of 1% was added;

[0063] Experimental hole: add the antitumor active component that embodiment 1 step (6) (b) gains

[0064] Cell culture:

[0065] at 5% CO 2 , 37° C., under saturated humidity, cultured human lung cancer Hep-G2 cells with DMEM (10% FBS, 1% PS) medium, and selected the cells growing in the logarithmic phase as experimental cells. After counting the cells, dilute it with medium to obtain a cell suspension of about 58,000 per milliliter.

[0066] Cell growth monitoring: put the cell real-time monitor in 5% CO 2 , 37°C saturated humidity incubator. Take an eight-well plate, add 150 μL of LDMEM medium to each well, put it into a real-time cell monitor for baseline, take out the eight-well plate after the baseline, and add 345 μL of diluted Hep-G2 cell suspension to each well to reach t...

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Abstract

The invention provides an extraction method and application of tobacco anti-tumor active components, and belongs to the technical field of processing and extraction of tobacco products. According to the method, tobacco raw materials are pretreated, operations such as normal-temperature extraction, high-temperature extraction, methanol extraction, acetone extraction and liquid chromatography separation are sequentially carried out on the pretreated tobacco raw materials, high-polarity solvent purified water, methanol and acetone are used as extraction solvents, the tobacco active components inthe tobacco raw materials can be fully extracted by adopting different extraction solvents and different extraction conditions, and the extraction content of the tobacco active components is increased. Experiments show that the target component has an anti-tumor function and can be developed into drugs for preventing and treating tumors.

Description

technical field [0001] The invention relates to the technical field of tobacco product processing and extraction, in particular to an extraction method and application of tobacco antitumor active components. Background technique [0002] Tobacco, also known as fairy grass, is a traditional Chinese medicinal material. Its inorganic components include water and mineral elements; organic components mainly include sugars, alkaloids, heterocyclics, pigments, phenols, terpenes, organic acids, lipids Substances, alcohols, aldehydes and ketones, etc. Among them, the organic components in tobacco have various functions such as antibacterial, antiviral, antitumor, antioxidant activity, and scavenging free radicals in the body. [0003] At present, in order to realize the extraction of active components of tobacco, supercritical extraction, Soxhlet extraction, and ultrasonic extraction are generally used. Absolute ethanol, n-hexane, acetone, ethyl acetate, petroleum ether, and dichlo...

Claims

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Application Information

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IPC IPC(8): A61K36/81A61P35/00
Inventor 王丰苏贤坤赵瑞娟曹军赵太均明株
Owner GUIZHOU TOBACCO SCI RES INST
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