46results about How to "Increased extraction content" patented technology

The invention discloses lithocarpus litseifolius total flavone extracted by using plant lithocarpus litseifolius as a raw material to develop a new hypoglycemic medicament, and provides a method for preparing the lithocarpus litseifolius total flavone. The purity of the lithocarpus litseifolius total flavone prepared by the method can reach over 80 percent, the phloridzin content reaches over 65 percent, the process is simple and has low energy consumption, and the technological condition is reasonable, stable and feasible. A hypoglycemic capsule developed by using the lithocarpus litseifolius total flavone can provide a convenient, acceptant and long-term taking oral hypoglycemic preparation for diabetics.

The invention provides a tobacco active ingredient, an extraction method and an application thereof, and belongs to the technical field of tobacco product processing and extraction. Through pretreating tobacco raw materials, and sequentially carrying out normal temperature extraction on the pretreated tobacco raw materials, High temperature extraction, methanol extraction three times, and purifiedwater using high polar solvent, methanol as the extraction solvent, through the use of different extraction solvents and different extraction conditions, can ensure that the tobacco active ingredients in tobacco raw materials can be fully extracted, improve the extraction content of tobacco active ingredients. It is found that the target component has antibacterial and anti-tumor functions and isa low toxicity product. Therefore, it has the development potential of antibacterial and anticancer drugs, and provides a theoretical basis for new drug research and development.

The invention discloses a double-component medicine for treating fatty liver and a preparation method thereof. The invention mainly solves the problem that the existing medicine for treating fatty liver is not suitable for long-term taking and can not simultaneously achieve stable effect, high application safety and small adverse reaction. The double-component medicine disclosed by the invention is prepared from a component A and a component B, wherein the component A is prepared from combined spicebush root extract, rhizoma corydalis extract, pseudo-ginseng powder, radix clematidis extract, black monkshood, eucommia extract and the like; and the component B is prepared from radix rehmanniae preparata extract, safflower extract, radix bupleuri extract, radix asparagi extract, radix ophiopogonis extract, radix cyathulae extract and the like. The invention also provides a preparation method of the double-component medicine. The invention is suitable for long-term taking, and has the advantages of stable effect, high application safety, small adverse reaction and the like.

The invention provides vegetable salt containing Chinese mahogany seed extract. The vegetable salt containing the Chinese mahogany seed extract comprises the following raw materials in parts by weight: 2-4 parts of Chinese mahogany seed extract and 6-8 parts of suaeda salsa vegetable-salt; the Chinese mahogany seed extract is characterized by having Chinese mahogany seed total flavonoid content of 0.53-0.61mg / g and gallic acid content of 0.42-0.53mg / g; and the suaeda salsa vegetable-salt is characterized by having total flavonoid content of 2.6-2.7mg / g and Fe content of 690-700mg / g. The invention further provides a preparation method of the vegetable salt containing the Chinese mahogany seed extract. The preparation method comprises a step of carrying out continuous countercurrent tube-type ultrasonic extraction. The vegetable salt containing the Chinese mahogany seed extract prepared by the preparation method has the effects of treating migraine, and is free of local irritation to animals.

The invention discloses an agent to enrich franc free DNA in pregnant woman plasma and method, which comprises the following steps: making the agent include 0.5M EDTA(pH=8.0) and analytical pure methylene oxide; allocating 3-5 ml peripheral blood; adding into EDTA and analytical pure methylene oxide; making the final density at 0.5 M and 1ml / L; centrifuging 1600g under room temperature at 10 min in 24 h; transferring the plasma to a new centrifuge tube; repeating the steps; can being used to successive trials or -80 cold conservation. This invention can be used to miscellaneous brand DNA extraction, which can increase the extracting content of pregnant woman plasma apparently.

The invention provides a tobacco active component (TWS), an extraction method and an application of the tobacco active component and belongs to the technical field of processing and extraction of tobacco products. A tobacco material is pretreated, the pretreated tobacco material is subjected to extraction at the normal temperature, a high-polarity solvent purified water is used as an extraction solvent, and experiments find that the target component has an antibacterial function, thereby having antibacterial drug development potentials and providing theoretical basis for research and development of new drugs.

The invention relates to a traditional Chinese medicine oral liquid for treating viral diseases of livestock and poultry. The traditional Chinese medicine oral liquid is prepared by virtue of the following steps: firstly, extracting mulberry leaf, weeping forsythia capsule, honeysuckle, baikal skullcap root, coptis chinensis, liquorice root, heartleaf houttuynia herb, hawthorn fruit, garlic, root of sinkiang arnebia, white peony root, pangolin scales, Japanese flowering fern rhizome, ash bark and common anemarrhena rhizome to obtain a concentrated liquor; and secondly, extracting mint and root of lobed kudzuvine to obtain a distillate, and blending the concentrated liquor with the distillate to obtain the oral liquid. In the way, the extraction content of the mint oil and the puerarin can be greatly increased; besides, the root of lobed kudzuvine, the heartleaf houttuynia herb, the Japanese flowering fern rhizome and the common anemarrhena rhizome are taken as the monarch drugs, while the coptis chinensis, the baikal skullcap root, the white peony root, the pangolin scales, the honeysuckle and the mulberry leaf are taken as the ministerial drugs, the weeping forsythia capsule, the mint, the garlic and the ash bark are taken as the adjuvant drugs, and the liquorice root is taken as the conductant drug, all the components are matched with each other to achieve a synergistic effect; the traditional Chinese medicine oral liquid has the effects of inhibiting and killing viruses, regulating immune functions, inducing the production of interferon and resisting toxins, is capable of effectively treating viral diseases without drug residue and free from toxic and side effects, and does not cause drug resistance.

The invention provides a technology for preparing black tea by utilizing polyphenol oxidase in potato. The technology comprises the following steps that (1) leaching liquid is prepared; (2) polyphenoloxidase is extracted, in specific, the pre-treated potato is mixed with the leaching liquid and extracted, and polyphenol oxidase extract liquid is obtained; (3) fresh tea is taken and placed in a withering tank to be withered for 8-12 hours, the tea is turned over every 1-1.5 hours, and the polyphenol oxidase extract liquid obtained in the step (2) is sprayed on the tea at a time; (4) the tea isrolled; (5) the rolled tea is evenly and loosely stacked in a fermentation container to be fermented; and (6) the well fermented tea is sent into a drying machine to be dried, till the water contentof the tea is 5% or below, and the black tea is obtained. The high-quality black tea is obtained by adopting the technology, the contents of theaflavin and thearubigin in the black tea are greatly increased, the fermentation time of the black tea is obviously shortened, and the production efficiency is improved.

The invention discloses an ultrasonic and microwave dual-assisted extraction method of total flavonoids of passion flower seed cake. The method herein comprises the steps of drying passion flower seeds at 55-70 DEG C to constant weight, crushing, screening to obtain seed powder, adding petroleum ether, with mass-volume ratio of the seed powder to the petroleum ether controlled to 6:(42-58), carrying out reflux condensation at 85-95 DEG C for 70-85 min, degreasing, recycling the petroleum ether via evaporation concentration, collecting degreased seed cake, adding ethanol-water solution, ultrasonically extracting under the thermostatic water bath condition of 75-85 DEG C to obtain a mixed liquid, carrying out microwave extraction, and filtering to obtain total flavonoid extract. The vibrational action and cavitation effect of the ultrasonic extraction technique are integrally utilized; the wall-breaking and heating effects of the microwave extraction technique enable the extraction efficiency to be greatly improved; the extraction technique is simple, and the extracting solvent is pollution free; utilization value of passion flower can be maximized.

The invention provides an active component (TEP) of tobacco and an extraction method and application thereof, and belongs to the technical field of tobacco product processing and extraction. Accordingto the method, tobacco raw materials are pretreated, the pretreated tobacco raw materials are subjected to normal-temperature extraction, high-temperature extraction and ethanol extraction in sequence, high-polar solvent purified water and ethanol are used as an extraction solvent, it can be ensured that the active component in the tobacco raw materials can be fully extracted by adopting different extraction solvents and different extraction conditions, and the extraction content of the active component of tobacco is improved. An experiment shows that the target component has an antimicrobialfunction, thus has the potential of antimicrobial drug development, and provides a theoretical basis for the development of new drugs.

The invention discloses a traditional Chinese medicine composition for auxiliary treatment of diabetes and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition mainly comprises the following raw materials: eria corneri, root of arrowleaf abelmoschus, ficus esquiroliana, elaeagnus lanccolata Warb., stemmacantha carthamoides (Willd.) Dittrich, root of ficus erecta, codonacanthus pauciflorus, root of tuan linden, root of narrow-leaf rabdosina, aleuritopteris farinosa, bulbophyllum psychoon, schisandra micrantha, a balsam pear extract and an evening primrose extract. The traditional Chinese medicine and the preparation method aim to overcome defects in the traditional Chinese medicine technology; the effective ingredients of such Chinese herbs are extracted according to the advanced technology in the prior art, so that the effects of the traditional Chinese medicine can be better and more lasting; the traditional Chinese medicine has the efficacies of nourishing yin to clear heat, tonifying the kidney to enrich essence, activating blood circulation to dredge collaterals, benefiting qi to remove stasis, and nourishing yin to strengthen the spleen, can be used for radical body conditioning, has a remarkable effect on the auxiliary treatment of diabetes, and is short in treating course, high in treating efficiency, free of side effects, low in recurrence possibility, and very good in popularization sense.

The invention provides a preparation method of cannabidiol by using industrial hemp as a raw material, and belongs to the field of biotechnology. The specific scheme is as follows: the preparation method of cannabidiol by using industrial hemp as the raw material comprises the following steps: step 1: taking leaves at one-third or more of the upper end of industrial hemp plants, and performing oven-drying and crushing to obtain an industrial hemp powder; step two: using absolute ethanol and / or methanol as a solvent, extracting the industrial hemp powder to obtain a CBD crude extraction liquid;step 3: concentrating the CBD crude extraction liquid to obtain a CBD concentration liquid; step 4: performing oil bath treatment on the CBD concentration liquid, removing volatile matters, and thenadding ethanol and / or methanol into bottom matters for re-dissolving to obtain a CBD solution with a purity of 20%-30%; step 5: adding the CBD solution into a chromatographic column, and performing eluting with dichloromethane and / or n-hexane to obtain a CBD chromatographic liquid; and step 6: concentrating the CBD chromatographic liquid to obtain a CBD concentration liquid with a purity of 60%-80%.

The invention discloses a method for extracting and determining hexabromocyclododecanes in a plant-soil system. The method comprises the following steps: S1, pretreating a to-be-detected plant / soil sample; S2, adding the to-be-detected plant / soil sample into a filter paper barrel, adding 3 to 4 drops of normal hexane, and adding a small piece of degreasing cotton above the sample; S3, performing Soxhlet extraction for 12 to 16h by taking normal hexane-acetone of which the volume ratio is 1:1 as a solvent, performing reflux for 5 to 7 times every hour, evaporating an extracting solution until the extracting solution is nearly dried, adding concentrated sulfuric acid and normal hexane of which the volume ratio is (3 to 5):1 for dissolving, extracting liquid supernatant after centrifugation, performing solid-phase extraction on the liquid supernatant through a solid-phase extraction column, and collecting eluant; then evaporating and concentrating the eluant until the eluant is nearly dried, cleaning by using methyl alcohol, nitrogen-blowing the eluant until the eluant is dried, fixing volume by using the methyl alcohol, performing full and uniform mixing, and determining by using high performance liquid chromatography-mass spectrometry. The method has the advantages of high determination speed, accurate analysis result, low pretreatment cost and applicability to migration and evaluation of a hexabromocyclododecane isomer in the plant-soil system.

The invention relates to an extraction method of ginsenoside. By the extraction method, the conventional production technology is changed, the ginsenoside is extracted through biological fermentation,raw materials can be directly used without being dried, the energy consumption is low, the added value is high, and the extraction efficiency is high; with supercritical carbon dioxide as an extractant, the extraction method has the characteristics of environment friendliness, no toxicity, no residue and the like; and with beeswax as an entrainer, the extraction content of ginsenoside can be significantly increased.

The invention provides a preparation method of silicon dioxide, including the following steps: S1) mixing slag material with carbonate and carrying out high-temperature reaction to obtain a first intermediate product, wherein the slag material contains silica; S2) mixing the first product with an acid solution for acid leaching and then filtering to obtain filtrate; S3) drying the filtrate to obtain a second intermediate product; and S4) calcining and pickling the second intermediate product to obtain silica. Compared with the prior art, the preparation method has the advantages of taking theslag material containing silica as the raw material and preparing silica with high purity through carbonate activation, acid leaching and calcination, is simple and environmentally friendly, has highextract content, and reduces the production cost by taking extracted silica to prepare a novel silicon-based negative electrode material of an lithium ion battery.

A method for extracting the cyclocarya paliurus polysaccharide comprises the following steps of: (1) sequentially carrying out freeze-drying, crushing and sieving on raw materials; (2) degreasing, vacuumizing and filtering; (3) pulping and carrying out enzymolysis for the first time; (4) adding ethanol, performing ultrasonic treatment, and filtering to obtain residue; (5) performing enzymolysis for the second time; (6) refluxing and extracting, centrifuging and collecting filtrate and residue; (7) concentrating and distilling the filtrate under reduced pressure, adding ethanol, centrifuging, and collecting precipitate; (8) washing the precipitate and filtering to obtain a cyclocarya paliurus crude polysaccharide blank; (9) purifying the blank; (10) preparing cyclocarya paliurus crude polysaccharide aqueous solution, adding chloroform and n-butanol solution, taking supernatant, adding ethanol, centrifuging, collecting the precipitate, and freeze-drying to obtain cyclocarya paliurus polysaccharide. The method has the advantages that the operation method is simple, the extraction content of the cyclocarya paliurus polysaccharide is improved, the quality is greatly improved, and the preparation of the solid beverage by using the cyclocarya paliurus polysaccharide is beneficial to improving the economic value of the cyclocarya paliurus polysaccharide.

The invention provides a tobacco active component and an extraction method and application thereof and belongs to the technical field of tobacco product processing and extraction. Tobacco raw materials are sequentially subjected to three operations of normal temperature extraction, high temperature extraction and methanol extraction after being pretreated, high polar solvent purified water and methanol are used as extraction solvents, the tobacco active components in the tobacco raw materials can be fully extracted assuredly and extraction content of the tobacco active components is increasedby using different extraction solvents and different extraction conditions. Experiments show that the component has antibacterial and antitumor functions and is a low-toxicity product; the tobacco active component has the potential of antibacterial and antitumor drugs and provides a theoretical basis for the research and development of new drugs.

The invention relates to an extraction technology of capsaicin. Raw materials are directly used without being dried. The extraction technology of the capsaicin has the advantages of low energy consumption, high extraction yield and low potential safety hazards; supercritical methanol is used as an extractant so as to meet environmental requirements, and the extraction efficiency is high; beewax isused as a cosolvent, so as to obviously increase the extraction content of the capsaicin.

The invention discloses a method for purifying a membrane protein. The method comprises the following steps: inducing a target membrane protein to be expressed in host cells; crushing the host cells, centrifuging at a low speed of 490-1000g, taking a supernatant, centrifuging the supernatant at at a high speed of 30000g, and taking a precipitate; re-suspending the precipitate by using a buffer, then adding a detergent, shaking and dissolving, centrifuging, and taking a supernatant; and purifying by adopting affinity chromatography, eluting by using an eluent containing a buffer, inorganic salt, dodecyl-beta-D-maltoside, imidazole and glycerol, and carrying out ultrafiltration and centrifugation on the eluent to obtain the target membrane protein. Aiming at the technical problems of membrane protein denaturation, low target membrane protein extraction rate, complex operation, high cost and the like caused by a detergent in the existing membrane protein purification method, low-speed centrifugation and high-speed centrifugation are combined, and the detergent is selected, so that the content of the target membrane protein in total protein is effectively increased, and the membrane protein purification method provided by the invention is simple to operate, low in cost, high in extraction rate and wide in applicability.

The invention discloses a common-sage-herb general flavone extracting method. The common-sage-herb general flavone extracting method includes the following steps that (1) fresh common sage herbs are selected as raw materials, and drying, smashing and screening are conduced; (2) the obtained common sage herbs are soaked in ethyl alcohol, and are subjected to ultrasonic treatment; (3) extracting isconducted, and common-sage-herb crude extracting liquid is obtained; (4) adsorption resin is prepared; (5) the resin adsorbs the common-sage-herb crude extracting liquid; (6) elution and drying are conducted to obtain common-sage-herb general flavone; (7) a standard curve is drawn, and the average content of the general flavone of samples is calculated; (8) the antioxidant capacity of the generalflavone is measured. The common-sage-herb general flavone extracting method has the advantages that an operating method is simple, the extracting content of the common-sage-herb general flavone is increased, and the quality (comprising the high purity and good solubility and dispersity) is greatly improved; a solid beverage is prepared with the common-sage-herb general flavone, and the economic value of the common sage herbs can be improved.

The invention discloses a cantharidin content determination method, which comprises: crushing cantharides into 20-70 mesh cantharides powder, adding water, uniformly mixing the mixture, carrying out multiple ultrasonic extraction, carrying out enzymolysis, adding an acetone solution, carrying out ultrasonic extraction, recovering the acetone solution to obtain cantharidin, and determining the cantharidin content by using liquid chromatography. After the cantharides are crushed, the later extraction time can be greatly shortened, and the extraction content is high; in addition, filtration is performed after extraction each time, the impurity rate is low, and the extraction purity of cantharidin is improved. A mobile phase, a control solution and a test solution are designed, and chromatographic analysis and determination are carried out through cooperation. According to the method, the content of cantharidin can be accurately measured, the operation is simple, the repeatability is good, the recovery rate is high, and the cantharidin can be accurately quantified.

The invention provides an extraction method and application of an anti-tumor active component from tobacco. The method includes the following steps that tobacco raw materials are subjected to ultrafine crushing firstly and then subjected to normal-temperature extraction, high-temperature extraction, methanol extraction and acetone extraction in sequence, wherein a high polar solvent, purified water, methanol and acetone are used as extracting solvents in sequence; a concentrated extractum of tobacco acetone extract is taken and subjected to chromatographic separation through a preparative liquid phase, obtained components are screened by a cell activity test to obtain components higher in activity, the obtained components higher in activity are separated and screened by a cell activity test again so as to obtain the component highest in activity, and the component highest in activity refers to the anti-tumor active component. The method has good repeatability and the obtained anti-tumor active component can be used for preparing anti-tumor drugs.

The invention provides a method for preparing a conditioner accelerating seed germination. The method includes preparation of a Sargassum horneri extract. The method includes steps of cleaning fresh Sargassum horneri air sacs, stirring the air sacs to crush the air sacs, adding an extracting solvent, performing extraction at 30-60 DEG C under the function of an external magnetic field, performingvacuum concentration at 25-32 DEG C, performing standing for crystallization and performing water-phase concentration to obtain the Sargassum horneri extract, with the extracting solvent being methanol-water-vanillic acid-glutathione in a volume ratio of 15-25:1:0.5:0.1-0.2. The conditioner comprises 50-60% of the Sargassum horneri extract and 1-8% of a coumarin compound as effective components. The conditioner can regulate and control contents and the ratio of gibberellins and abscisic acid in plants through affecting activity of related enzymes in plant seed immature embryos, thus promotingseed germination.

The invention provides a method for determining hexabromodiphenyl in ambient air. Two kinds of hexabromodiphenyl in the ambient air are detected at the same time by combining accelerated solvent extraction with gas chromatography. The method comprises the following steps: (1) sample collection: continuously collecting ambient air samples at sampling points by adopting a novel ambient air sampler; (2) extracting hexabromodiphenyl in the sample by adopting an accelerated solvent extractor; (3) purifying a target object by using a solid-phase extraction small column; and (4) detecting the two hexabromobiphenyls in the sample by using a gas chromatographic method, and performing quantifying by using an external standard method. According to the method, two hexabromodiphenyl monomers are selected for an ambient air medium for the first time, and an air sample pretreatment method and a gas chromatography detection and analysis method are established.

The invention discloses a composition for tonifying spleen, eliminating dampness and losing weight as well as a preparation method and application thereof, and belongs to the technical field of functional foods. The composition is prepared from the following components in parts by weight: 0.5 to 10 parts of fresh rhizoma phragmitis, 0.05 to 1 part of poria cocos, 2 to 10 parts of malt, 0.1 to 8 parts of semen armeniacae amarae, 0.05 to 1 part of semen coicis, 0.5 to 10 parts of herba lophatheri, 0.5 to 10 parts of semen myristicae, 0.05 to 5 parts of semen raphani, 0.05 to 5 parts of liquorice root and 0.05 to 5 parts of fructus lycii. The 11 raw materials of the composition have the effects of diffusing the lung and benefiting qi in the upper jiao, tonifying the spleen and appetizing in the middle jiao and promoting the diuresis in the lower jiao, and the medicines are compatible to remove damp evil in the upper jiao, the middle jiao and the lower jiao, strengthen the transportation of the spleen and the stomach and promote fat metabolism, so that the effects of tonifying the spleen, eliminating dampness and losing weight are achieved.

The invention provides an extraction method and application of tobacco anti-tumor active components, and belongs to the technical field of processing and extraction of tobacco products. According to the method, tobacco raw materials are pretreated, operations such as normal-temperature extraction, high-temperature extraction, methanol extraction, acetone extraction and liquid chromatography separation are sequentially carried out on the pretreated tobacco raw materials, high-polarity solvent purified water, methanol and acetone are used as extraction solvents, the tobacco active components inthe tobacco raw materials can be fully extracted by adopting different extraction solvents and different extraction conditions, and the extraction content of the tobacco active components is increased. Experiments show that the target component has an anti-tumor function and can be developed into drugs for preventing and treating tumors.

The invention discloses a traditional Chinese medicine compound concentrated decoction with a cold prevention effect and a quality control method thereof. A preparation method of the traditional Chinese medicine compound concentrated decoction comprises the following steps: taking 15-30 parts of milkvetch roots, 9-18 parts of stir-fried largehead atractylodes rhizome, 6-12 parts of radix codonopsitis, 3-6 parts of cassia twigs, 2-4 parts of dried ginger, 2-4 parts of medicinal evodia fruits, 5-10 parts of perilla leaves and 6-12 parts of divaricate saposhnikovia roots; adding water of which the volume is 18 times the weight of the medicinal materials so as to soak the medicinal materials for 60min; and performing decoction for 210min, and performing concentration to 480mL so as to obtain the traditional Chinese medicine compound concentrated decoction. The invention screens out an optimal extraction method through a lot of experiments; the traditional Chinese medicine compound concentrated decoction is extracted by adding the water of which the volume is 18 times the weight of the medicinal materials to soak the medicinal materials for 60min, performing decoction for 210min and performing concentration until the volume is 480mL; the contents of active components including calycosin-7-glucoside, prim-o-glucosylcimifugin, 5-O-methylvisammioside and cinnamic acid are highest; andexcellent unexpected technical effects are achieved.

The invention discloses a method for extracting flavonoid compounds from persimmon leaves. The method comprises the following steps: A, picking the fresh persimmon leaves, rinsing and airing; B, crushing the aired persimmon leaves, and sieving; C, adding the sieved crushed persimmon leaf material into a mixed aqueous solution of ethanol, acetic acid and formic acid, and performing ultrasonic extraction twice to obtain a primary extract; D, adding an aqueous sodium hydroxide solution into the primary extract, and performing microwave extraction twice to obtain a secondary extract; E, performingultrafiltration on the secondary extract, and then performing vacuum concentration to obtain a concentrate; F, drying the concentrate at low temperature to obtain the flavonoid compounds. The operation method adopted in the invention is simple, easy to implement and low in cost; according to the method, the content of the extracted flavonoid compounds can be increased.

The invention discloses a heparin sodium salt-free extraction preparation process, which comprises the following steps of: intestinal mucosa preparation: crushing animal small intestines into particles, adding clear water for mixing according to the ratio of the animal small intestines to water being 1: (12-18), adding the mixture into a stirrer, and stirring for 10-15 minutes to obtain a primary mucosa liquid; and PH adjustment: adding an alkaline blending liquid into the primary mucosa liquid, and raising the PH of the primary mucosa liquid to 8.6-9.4. The method has the following beneficial effects that the pH value of the animal small intestines is adjusted, then a compound enzyme solution is added to carry out enzymolysis on the mucous membrane liquid to enable heparin sodium contained in the animal small intestines to be fully subjected to enzymolysis, and then ultrasonic enzymolysis and quaternary ammonium salt extraction are carried out to enable the extraction content of the heparin sodium to reach the highest. Compared with the prior art, the heparin sodium extracted by the process has the advantages that the content of the heparin sodium finally obtained from every 2000 animal small intestines reaches 1075 g, the yield is high, and the quality is stable.