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Technology for preparing black tea by utilizing polyphenol oxidase in potato

A technology of polyphenol oxidase and potatoes, which is applied in tea treatment before extraction, etc., can solve the problems of low extraction efficiency of polyphenol oxidase, the method has not been fully reported, and is limited to laboratory research, so as to shorten the time required for fermentation. Time, quality improvement, and high extraction efficiency

Inactive Publication Date: 2019-01-15
CHENGDU VOCATIONAL COLLEGE OF AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, many studies are dedicated to how to increase the content of thearubigins and theaflavins in tea to improve the quality of tea. Studies have found that a polyphenol oxidase can be used to oxidize tea polyphenols and increase the content of thearubigins in tea. and theaflavin content to improve the quality of tea, but the current research is still in its infancy, limited to laboratory research, and no breakthroughs have been made
However, the extraction of exogenous polyphenol oxidase is affected by many conditions, resulting in low extraction efficiency of polyphenol oxidase, and its method for improving tea quality has not been fully reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A process for preparing black tea, comprising the following preparation steps:

[0030] (1) Preparation of leaching solution: configure citric acid buffer solution, the concentration of the citrate buffer solution is 0.5mol / L, and the pH is 5.2, and the buffer solution is mixed with isopropanol and CTAB to prepare the leaching solution , the concentration of isopropanol in the leaching solution is 3g / 100mL, and the concentration of CTAB is 50mmol / L;

[0031] (2) Extraction of polyphenol oxidase: take fresh potatoes, wash, smash and make potato pulp, pass the potato pulp through 100-mesh gauze to obtain a filtrate, and mix the filtrate with the extract obtained in step (1) to carry out For extraction, the mass ratio of potato filtrate and extract is 1:5, the extraction temperature is 20°C, and the extraction time is 10min. After the extraction is completed, pass through 200-mesh gauze, and then centrifuge at a speed of 10,000 rpm for 5min. The supernatant is obtained to...

Embodiment 2

[0038] A process for preparing black tea, comprising the following preparation steps:

[0039] (1) Preparation of leaching solution: configure citric acid buffer solution, the concentration of the citrate buffer solution is 1.2mol / L, and the pH is 7.8, and the buffer solution is mixed with isopropanol and CTAB to prepare the leaching solution , the concentration of isopropanol in the leaching solution is 6g / 100mL, and the concentration of CTAB is 100mmol / L;

[0040] (2) Extraction of polyphenol oxidase: take fresh potatoes, wash, smash and make potato pulp, pass the potato pulp through 100-mesh gauze to obtain a filtrate, and mix the filtrate with the extract obtained in step (1) to carry out For extraction, the mass ratio of potato filtrate and extract is 1:8, the extraction temperature is 30°C, and the extraction time is 30min. After the extraction is completed, pass through 200-mesh gauze, and then centrifuge at a speed of 15,000 rpm for 5min. The supernatant is obtained t...

Embodiment 3

[0047] A process for preparing black tea, comprising the following preparation steps:

[0048] (1) Preparation of leaching solution: configure citric acid buffer solution, the concentration of the citric acid buffer solution is 0.8mol / L, and the pH is 6.5, and the buffer solution is mixed with isopropanol and CTAB to prepare the leaching solution , the concentration of isopropanol in the leaching solution is 5g / 100mL, and the concentration of CTAB is 80mmol / L;

[0049] (2) Extraction of polyphenol oxidase: take fresh potatoes, wash, smash and make potato pulp, pass the potato pulp through 100-mesh gauze to obtain filtrate, and mix the filtrate with the extract obtained in step (1) to carry out For extraction, the mass ratio of potato filtrate and extract is 1:6, the extraction temperature is 25°C, and the extraction time is 20min. After the extraction is completed, pass through 200-mesh gauze, and then centrifuge at a speed of 12,000 rpm for 5min. The supernatant is obtained ...

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PUM

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Abstract

The invention provides a technology for preparing black tea by utilizing polyphenol oxidase in potato. The technology comprises the following steps that (1) leaching liquid is prepared; (2) polyphenoloxidase is extracted, in specific, the pre-treated potato is mixed with the leaching liquid and extracted, and polyphenol oxidase extract liquid is obtained; (3) fresh tea is taken and placed in a withering tank to be withered for 8-12 hours, the tea is turned over every 1-1.5 hours, and the polyphenol oxidase extract liquid obtained in the step (2) is sprayed on the tea at a time; (4) the tea isrolled; (5) the rolled tea is evenly and loosely stacked in a fermentation container to be fermented; and (6) the well fermented tea is sent into a drying machine to be dried, till the water contentof the tea is 5% or below, and the black tea is obtained. The high-quality black tea is obtained by adopting the technology, the contents of theaflavin and thearubigin in the black tea are greatly increased, the fermentation time of the black tea is obviously shortened, and the production efficiency is improved.

Description

technical field [0001] The invention relates to a process for preparing black tea, in particular to a process for preparing black tea by utilizing polyphenol oxidase in potatoes. Background technique [0002] Black tea is a kind of tea drink that people love. It is the tea with the largest output and the largest number of consumers in the world, accounting for about 80% of the world's total tea trade. China is the first country in the world to produce and process black tea. However, because my country's tea tree cultivation, tea processing technology and processing equipment lag behind foreign countries, the aroma quality of black tea in my country is not as good as that of Kenya, Sri Lanka, India and other countries, and the taste and quality components are also far away. Lower than these countries, the share of my country's black tea in the international market is less than 5%. At present, it mainly occupies a larger sales market in China. [0003] Research on black tea sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23F3/10
CPCA23F3/10
Inventor 李慧熊丙全阳淑练华山邹盼红杨铱彭玲莉史伟蒋跃军
Owner CHENGDU VOCATIONAL COLLEGE OF AGRI SCI & TECH
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