Technology for preparing black tea by utilizing polyphenol oxidase in potato
A technology of polyphenol oxidase and potatoes, which is applied in tea treatment before extraction, etc., can solve the problems of low extraction efficiency of polyphenol oxidase, the method has not been fully reported, and is limited to laboratory research, so as to shorten the time required for fermentation. Time, quality improvement, and high extraction efficiency
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Embodiment 1
[0029] A process for preparing black tea, comprising the following preparation steps:
[0030] (1) Preparation of leaching solution: configure citric acid buffer solution, the concentration of the citrate buffer solution is 0.5mol / L, and the pH is 5.2, and the buffer solution is mixed with isopropanol and CTAB to prepare the leaching solution , the concentration of isopropanol in the leaching solution is 3g / 100mL, and the concentration of CTAB is 50mmol / L;
[0031] (2) Extraction of polyphenol oxidase: take fresh potatoes, wash, smash and make potato pulp, pass the potato pulp through 100-mesh gauze to obtain a filtrate, and mix the filtrate with the extract obtained in step (1) to carry out For extraction, the mass ratio of potato filtrate and extract is 1:5, the extraction temperature is 20°C, and the extraction time is 10min. After the extraction is completed, pass through 200-mesh gauze, and then centrifuge at a speed of 10,000 rpm for 5min. The supernatant is obtained to...
Embodiment 2
[0038] A process for preparing black tea, comprising the following preparation steps:
[0039] (1) Preparation of leaching solution: configure citric acid buffer solution, the concentration of the citrate buffer solution is 1.2mol / L, and the pH is 7.8, and the buffer solution is mixed with isopropanol and CTAB to prepare the leaching solution , the concentration of isopropanol in the leaching solution is 6g / 100mL, and the concentration of CTAB is 100mmol / L;
[0040] (2) Extraction of polyphenol oxidase: take fresh potatoes, wash, smash and make potato pulp, pass the potato pulp through 100-mesh gauze to obtain a filtrate, and mix the filtrate with the extract obtained in step (1) to carry out For extraction, the mass ratio of potato filtrate and extract is 1:8, the extraction temperature is 30°C, and the extraction time is 30min. After the extraction is completed, pass through 200-mesh gauze, and then centrifuge at a speed of 15,000 rpm for 5min. The supernatant is obtained t...
Embodiment 3
[0047] A process for preparing black tea, comprising the following preparation steps:
[0048] (1) Preparation of leaching solution: configure citric acid buffer solution, the concentration of the citric acid buffer solution is 0.8mol / L, and the pH is 6.5, and the buffer solution is mixed with isopropanol and CTAB to prepare the leaching solution , the concentration of isopropanol in the leaching solution is 5g / 100mL, and the concentration of CTAB is 80mmol / L;
[0049] (2) Extraction of polyphenol oxidase: take fresh potatoes, wash, smash and make potato pulp, pass the potato pulp through 100-mesh gauze to obtain filtrate, and mix the filtrate with the extract obtained in step (1) to carry out For extraction, the mass ratio of potato filtrate and extract is 1:6, the extraction temperature is 25°C, and the extraction time is 20min. After the extraction is completed, pass through 200-mesh gauze, and then centrifuge at a speed of 12,000 rpm for 5min. The supernatant is obtained ...
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