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Tobacco active component and extraction method and application thereof

A technology of active components and extraction methods, which is applied in the field of tobacco product processing and extraction, and can solve the problems of low content of tobacco active components, toxicity, and high requirements

Inactive Publication Date: 2019-07-05
GUIZHOU TOBACCO SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, low-polarity solvents such as petroleum ether, n-hexane, medium-polarity solvents acetone, and methylene chloride are mostly volatile, flammable, and toxic solvents, which have high requirements for industrial production equipment and personnel, and the obtained tobacco activity The content of components is small, which greatly limits the further research and application of tobacco active components

Method used

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  • Tobacco active component and extraction method and application thereof
  • Tobacco active component and extraction method and application thereof
  • Tobacco active component and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 of the present invention provides a kind of extraction method of tobacco active component, comprises the following steps:

[0041] (1) Pretreatment.

[0042] Weigh 30Kg of tobacco raw material, place it in an oven for drying, the drying temperature is 50°C, the drying time is 4h, and take out after drying until the moisture content of the tobacco is less than 5%; take the dried tobacco raw material, and use a 50-mesh screen After pulverizing by the pulverizer, put it into a pulverizer with a 200-mesh sieve for superfine pulverization to obtain superfine tobacco powder.

[0043] (2) extraction with normal temperature water.

[0044] Weigh 30Kg of tobacco superfine powder into a 200L extraction tank, add 100Kg of purified water, stir and extract at room temperature for 0.5h; of sediment.

[0045] (3) High temperature water extraction.

[0046] Weigh 25Kg of the precipitate in step (2) and add it to a 100L concentrated extraction tank; add 60Kg of purified...

Embodiment 2

[0050] Embodiment 2 of the present invention provides a kind of extraction method of tobacco active component, comprises the following steps:

[0051] (1) Pretreatment.

[0052] Weigh 40Kg of tobacco raw material, place it in an oven for drying, the drying temperature is 55°C, the drying time is 6h, and take out after drying until the moisture content of the tobacco is less than 5%; take the dried tobacco raw material, and use a 60-mesh screen After being pulverized by a pulverizer, put it into a pulverizer with a 300-mesh screen for superfine pulverization to obtain superfine tobacco powder.

[0053] (2) extraction with normal temperature water.

[0054] Weigh 40Kg of tobacco superfine powder into a 200L extraction tank, add 120Kg of purified water, stir and extract at room temperature for 1.5h; transfer to a centrifuge bottle after stirring and extraction, and centrifuge at 10°C for 1h at a speed of 5000rpm / min; collect centrifuged Precipitate.

[0055] (3) High temperatu...

Embodiment 3

[0060] Embodiment 3 of the present invention provides a kind of extraction method of tobacco active component, comprises the following steps:

[0061] (1) Pretreatment.

[0062] Weigh 50Kg of tobacco raw material, place it in an oven for drying, the drying temperature is 60°C, the drying time is 8h, and take out after drying until the moisture content of the tobacco is less than 5%. Take the dried tobacco raw material and use an 80-mesh screen After pulverizing by the pulverizer, put it into a pulverizer with a 400-mesh sieve for superfine pulverization treatment to obtain superfine tobacco powder.

[0063] (2) extraction with normal temperature water.

[0064] Weigh 50Kg of tobacco superfine powder into a 200L extraction tank, add 120Kg of purified water, stir and extract at room temperature for 0.5h; of sediment.

[0065] (3) High temperature water extraction.

[0066] Weigh 45Kg of the precipitate in step (2) and add it to a 100L concentrated extraction tank; add 60Kg o...

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Abstract

The invention provides a tobacco active component and an extraction method and application thereof and belongs to the technical field of tobacco product processing and extraction. Tobacco raw materials are sequentially subjected to three operations of normal temperature extraction, high temperature extraction and methanol extraction after being pretreated, high polar solvent purified water and methanol are used as extraction solvents, the tobacco active components in the tobacco raw materials can be fully extracted assuredly and extraction content of the tobacco active components is increasedby using different extraction solvents and different extraction conditions. Experiments show that the component has antibacterial and antitumor functions and is a low-toxicity product; the tobacco active component has the potential of antibacterial and antitumor drugs and provides a theoretical basis for the research and development of new drugs.

Description

technical field [0001] The invention relates to the technical field of tobacco product processing and extraction, in particular to a tobacco active component, an extraction method and an application thereof. Background technique [0002] Tobacco, also known as fairy grass, is a traditional Chinese medicinal material. Its inorganic components include water and mineral elements; organic components mainly include sugars, alkaloids, heterocyclics, pigments, phenols, terpenes, organic acids, lipids Substances, alcohols, aldehydes and ketones, etc. Among them, the organic components in tobacco have various functions such as antibacterial, antiviral, antitumor, antioxidant activity, and scavenging free radicals in the body. [0003] At present, in order to realize the extraction of active components of tobacco, supercritical extraction, Soxhlet extraction, and ultrasonic extraction are generally used. Absolute ethanol, n-hexane, acetone, ethyl acetate, petroleum ether, and dichlo...

Claims

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Application Information

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IPC IPC(8): A61K36/81A61P35/00A61P31/04A61P31/10
CPCA61K36/81A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61P31/04A61P31/10A61P35/00
Inventor 杨慧苏贤坤王丰曹军陈梅张时山
Owner GUIZHOU TOBACCO SCI RES INST
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