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Active component (TEP) of tobacco and extraction method and application thereof

A technology of active components and extraction methods, which is applied in the field of tobacco product processing and extraction, can solve problems such as flammability, toxicity, and volatility, and achieve the effects of simple preparation methods, broad application prospects, and increased extraction content

Inactive Publication Date: 2019-07-12
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, low-polarity solvents such as petroleum ether, n-hexane, medium-polarity solvents acetone, and methylene chloride are mostly volatile, flammable, and toxic solvents, which have high requirements for industrial production equipment and personnel, and the obtained tobacco activity The content of components is small, which greatly limits the further research and application of tobacco active components

Method used

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  • Active component (TEP) of tobacco and extraction method and application thereof
  • Active component (TEP) of tobacco and extraction method and application thereof
  • Active component (TEP) of tobacco and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the extraction of tobacco antibacterial active component

[0031] 1. The extraction steps of tobacco antibacterial active components are as follows:

[0032] (1) Pretreatment: drying the tobacco raw material, coarsely pulverizing the dried tobacco raw material, and finally superfine pulverizing to obtain superfine tobacco powder;

[0033] (2) Water extraction at normal temperature: add extraction solvent in tobacco superfine powder, carry out extraction operation under normal temperature condition; Carry out centrifugation to the tobacco superfine powder after extraction operation, obtain tobacco normal temperature water extraction sediment and supernatant, will supernatant The clear liquid is stored at low temperature and freeze-dried to obtain the freeze-dried powder of the tobacco supernatant extracted with normal temperature water; the extraction solvent in step (2) is purified water;

[0034] (3) High-temperature water extraction: add an extraction s...

Embodiment 2

[0036] Embodiment 2: The experiment that tobacco active component inhibits the growth of fungus-Cordyceps fungus

[0037] 1. The purpose of the experiment:

[0038] Through the antibacterial experiment, the antibacterial efficacy of tobacco extract can be determined.

[0039] 2. Experimental method:

[0040] agar punch method

[0041] 3. Experimental principle:

[0042] The antibacterial substance diffuses and penetrates from the hole to the agar medium, and affects the growth and reproduction of the bacteria through the inhibitory effect on the test bacteria, so that the colony forms a bacteriostatic zone, and the antibacterial ability of the antibacterial substance is determined according to the size of the bacteriostatic zone. This test is suitable for the identification of bacteriostatic agents and dissolution bacteriostatic products. This test is suitable for the identification of bacteriostatic agents and dissolution bacteriostatic products.

[0043] 4. Experimental...

Embodiment 3

[0052]Embodiment 3: the experiment that tobacco active component inhibits the growth of bacteria-Escherichia coli

[0053] 1. The purpose of the experiment:

[0054] Through the antibacterial experiment, the antibacterial efficacy of tobacco extract can be determined.

[0055] 2. Experimental method:

[0056] agar punch method

[0057] 3. Experimental principle:

[0058] The antibacterial substance diffuses and penetrates from the hole to the agar medium, and affects the growth and reproduction of the bacteria through the inhibitory effect on the test bacteria, so that the colony forms a bacteriostatic zone, and the antibacterial ability of the antibacterial substance is determined according to the size of the bacteriostatic zone. This test is suitable for the identification of bacteriostatic agents and dissolution bacteriostatic products.

[0059] 4. Experimental strains:

[0060] Escherichia coli

[0061] 5. Experimental steps:

[0062] (1) Prepare solid medium: yeast...

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Abstract

The invention provides an active component (TEP) of tobacco and an extraction method and application thereof, and belongs to the technical field of tobacco product processing and extraction. Accordingto the method, tobacco raw materials are pretreated, the pretreated tobacco raw materials are subjected to normal-temperature extraction, high-temperature extraction and ethanol extraction in sequence, high-polar solvent purified water and ethanol are used as an extraction solvent, it can be ensured that the active component in the tobacco raw materials can be fully extracted by adopting different extraction solvents and different extraction conditions, and the extraction content of the active component of tobacco is improved. An experiment shows that the target component has an antimicrobialfunction, thus has the potential of antimicrobial drug development, and provides a theoretical basis for the development of new drugs.

Description

technical field [0001] The invention relates to the technical field of tobacco product processing and extraction, in particular to a tobacco active component (TEP), an extraction method and an application thereof. Background technique [0002] Tobacco, also known as fairy grass, is a traditional Chinese medicinal material. Its inorganic components include water and mineral elements; organic components mainly include sugars, alkaloids, heterocyclics, pigments, phenols, terpenes, organic acids, lipids Substances, alcohols, aldehydes and ketones, etc. Among them, the organic components in tobacco have various functions such as antibacterial, antiviral, antitumor, antioxidant activity, and scavenging free radicals in the body. [0003] At present, in order to realize the extraction of active components of tobacco, supercritical extraction, Soxhlet extraction, and ultrasonic extraction are generally used. Absolute ethanol, n-hexane, acetone, ethyl acetate, petroleum ether, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/81A61P31/04A61P31/10
CPCA61K36/81A61K2236/15A61K2236/331A61K2236/333A61K2236/39A61P31/04A61P31/10
Inventor 王丰杨慧苏贤坤曹军张时山聂开美
Owner GUIZHOU TOBACCO SCI RES INST
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