Method for determining content of cantharidin
A method for measuring cantharidin, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that the detection accuracy needs to be further improved, the steps are cumbersome, time-consuming, etc., and achieve good recovery rate, good repeatability, and impurity content. low rate effect
Pending Publication Date: 2021-11-09
JIANGSU SIHUAN BIOENGINEERING PHARM CO LTD
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Problems solved by technology
However, the current method for the determination of cantharidin content is cumbersome and time-consuming, and the detection accuracy needs to be further improved.
Method used
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Embodiment 1
[0065] Reagents and materials:
[0066] Methanol (analytical grade or better);
[0067] Purified water (ChP grade);
[0068] Acetone solution;
[0069] Protease;
[0070] lye, preferably sodium hydroxide;
[0071] Cantharidin reference substance;
[0072] Cantharidin samples.
[0073] equipment:
[0074] Liquid chromatography;
[0075] Chromatographic column: Agilent C18, 4.6×250mm, 5μm or equivalent column;
[0076] Pure water preparation instrument;
[0077] Analytical Balances;
[0078] 10ml volumetric flask.
[0079] Chromatographic conditions:
[0080] Chromatographic column: Agilent C18, 250mm×4.6mm, 5μm or equivalent column
[0081] Column temperature: 25°C
[0082] Mobile phase: methanol-water (23:77)
[0083] Flow rate: 1.0ml / min
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Abstract
The invention discloses a cantharidin content determination method, which comprises: crushing cantharides into 20-70 mesh cantharides powder, adding water, uniformly mixing the mixture, carrying out multiple ultrasonic extraction, carrying out enzymolysis, adding an acetone solution, carrying out ultrasonic extraction, recovering the acetone solution to obtain cantharidin, and determining the cantharidin content by using liquid chromatography. After the cantharides are crushed, the later extraction time can be greatly shortened, and the extraction content is high; in addition, filtration is performed after extraction each time, the impurity rate is low, and the extraction purity of cantharidin is improved. A mobile phase, a control solution and a test solution are designed, and chromatographic analysis and determination are carried out through cooperation. According to the method, the content of cantharidin can be accurately measured, the operation is simple, the repeatability is good, the recovery rate is high, and the cantharidin can be accurately quantified.
Description
technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a method for determining the content of cantharidin. Background technique [0002] Mylabris, aliased as coriander green, piebald hair, etc., is the dry worm body of Mylabris phalerataPallas or Mylabris cichorii Linnaeus, which is a cold and pungent taste, and is a highly toxic Chinese herbal medicine. Mylabris can erode dead muscles when used externally, apply scabies and malignant sores, and when taken orally, it has the effects of attacking poison, dispelling blood stasis and dispelling stagnation, and antitumor. Modern medical research has proved that the medicinal component of mylabris is mainly cantharidin, which is a natural active substance with special effects. [0003] In addition to important clinical applications such as anti-malignant tumors, cantharidin can also be added to hair growth cosmetics, which can relieve skin itching, improve local neurot...
Claims
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IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/74
CPCG01N30/02G01N30/06G01N30/54G01N30/74
Inventor 江永红丁月萍刘红刘蕾陶冬梅
Owner JIANGSU SIHUAN BIOENGINEERING PHARM CO LTD
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