Ultrasonic and microwave dual-assisted extraction method of total flavonoids of passion flower seed cake

A technology of passion flower seed oil and auxiliary extraction, which is applied in the direction of medical formula, medical preparations containing active ingredients, plant raw materials, etc., and can solve the problems of low flavonoid extraction content, too small flavonoid concentration difference, unfavorable flavonoid dissolution, etc. , to achieve the effects of high flavonoid extraction content, increased extraction efficiency, and no pollution to the extraction solvent

Active Publication Date: 2019-07-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the solid-liquid ratio is too large, the concentration difference of flavonoids between the raw material and the solvent is too small, and the mass transfer driving force is too low, which is not conducive to the dissolution of flavonoids, resulting in a low extraction content of flavonoids

Method used

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  • Ultrasonic and microwave dual-assisted extraction method of total flavonoids of passion flower seed cake
  • Ultrasonic and microwave dual-assisted extraction method of total flavonoids of passion flower seed cake
  • Ultrasonic and microwave dual-assisted extraction method of total flavonoids of passion flower seed cake

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Experimental program
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Effect test

Embodiment 1

[0045] Embodiment 1 A kind of method that adopts ultrasonic wave, microwave double auxiliary extraction total flavonoids of passion fruit seed oil meal

[0046] (1) pulverizing: the passionflower seed is dried to constant weight at 55 DEG C, the dried passionflower seed is pulverized with a pulverizer, crosses a 0.250mm sieve, and obtains the seed powder;

[0047] (2) Degreasing: Weigh 6.0 g of seed powder and put it into a filter paper tube, add 42 mL of petroleum ether into a quartz cup, reflux at 95°C for 70 minutes to degrease, recover petroleum ether by rotary evaporation, and collect the degreased seed oil meal;

[0048](3) Ultrasonic extraction: Weigh 1.0g of the above-mentioned seed oil meal, add ethanol-water solution with a volume fraction of ethanol of 50% according to the ratio of material to liquid 1:20g / mL, set the ultrasonic power to 200w, and extract in a constant temperature water bath at 80°C for 60min , to obtain a mixture;

[0049] (4) Microwave extraction...

Embodiment 2

[0050] Embodiment 2 A kind of method that adopts ultrasonic wave, microwave double auxiliary extraction total flavonoids of passionflower seed oil meal

[0051] (1) pulverizing: the passionflower seed is dried to constant weight at 70 DEG C, the dried passionflower seed is pulverized with a pulverizer, and crosses a 0.425mm sieve to obtain the seed powder;

[0052] (2) Degreasing: Weigh 6.0 g of seed powder and put it into a filter paper tube, add 58 mL of petroleum ether into a quartz cup, reflux at 85°C for 85 minutes to degrease, recover petroleum ether by rotary evaporation, and collect the degreased seed oil meal;

[0053] (3) Ultrasonic extraction: Weigh 1.0 g of the above-mentioned seed oil meal, add an ethanol solution with an ethanol volume fraction of 90% according to a material-to-liquid ratio of 1:60 g / mL, set the ultrasonic power to 500w, and extract in a constant temperature water bath at 80°C for 10 minutes. get mixed solution;

[0054] (4) Microwave extraction...

Embodiment 3

[0055] Embodiment 3 A kind of method that adopts ultrasonic wave, microwave double auxiliary extraction total flavonoids of passionflower seed oil meal

[0056] (1) pulverizing: the passionflower seed is dried to constant weight at 60 DEG C, the dried passionflower seed oil meal is pulverized with a pulverizer, and crosses a 0.300mm sieve to obtain the seed powder;

[0057] (2) Degreasing: Weigh 6.0 g of seed powder and put it into a filter paper tube, add 50 mL of petroleum ether into a quartz cup, condense and reflux at 85°C for 70 minutes to degrease, recover petroleum ether by rotary evaporation, and collect the degreased seed oil meal;

[0058] (3) Ultrasonic extraction: Weigh 1.0 g of the above-mentioned seed oil meal, add an ethanol solution with an ethanol volume fraction of 80% according to a material-to-liquid ratio of 1:50 g / mL, set the ultrasonic power to 300w, and extract in a constant temperature water bath at 80°C for 30 minutes. The number of times of ultrasoni...

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Abstract

The invention discloses an ultrasonic and microwave dual-assisted extraction method of total flavonoids of passion flower seed cake. The method herein comprises the steps of drying passion flower seeds at 55-70 DEG C to constant weight, crushing, screening to obtain seed powder, adding petroleum ether, with mass-volume ratio of the seed powder to the petroleum ether controlled to 6:(42-58), carrying out reflux condensation at 85-95 DEG C for 70-85 min, degreasing, recycling the petroleum ether via evaporation concentration, collecting degreased seed cake, adding ethanol-water solution, ultrasonically extracting under the thermostatic water bath condition of 75-85 DEG C to obtain a mixed liquid, carrying out microwave extraction, and filtering to obtain total flavonoid extract. The vibrational action and cavitation effect of the ultrasonic extraction technique are integrally utilized; the wall-breaking and heating effects of the microwave extraction technique enable the extraction efficiency to be greatly improved; the extraction technique is simple, and the extracting solvent is pollution free; utilization value of passion flower can be maximized.

Description

technical field [0001] The invention belongs to the technical field of plant natural component extraction. Specifically, it relates to a method for extracting total flavonoids from passion flower seed oil meal with ultrasonic and microwave assistance. Background technique [0002] Passiflora edulis Sims (Passiflora edulis Sims) is a vine of the genus Passiflora in the Passiflora family. It is planted on a large scale in Yunnan, Guangdong, Guangxi and other provinces in my country. Its fruit juice has a pleasant fragrance and unique flavor, and occupies an important position in the beverage market. status. Passion fruit produces seeds accounting for about 11% of the whole fruit after the juice is extracted, and the oil content of the seeds is about 20%. At present, the research on the utilization of passion fruit seeds at home and abroad mainly focuses on the development of seed oil. The research on the active ingredients of purple passion fruit seeds shows that it contains ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K131/00
CPCA61K36/185A61K2236/10A61K2236/15A61K2236/333A61K2236/39
Inventor 黄苇李焱
Owner SOUTH CHINA AGRI UNIV
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