Heparin sodium salt-free extraction preparation process

A preparation process, the technology of heparin sodium, is applied in the field of heparin sodium salt-free extraction and preparation process, which can solve the problems of environmental impact, high-salt wastewater difficult to discharge, etc., and achieve the effects of reducing treatment cost, good extraction effect and high yield

Pending Publication Date: 2022-02-08
潢川县鹏升畜产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This extraction method makes it difficult to discharge high-salt wastewater without pollution, which will have a great impact on the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] This embodiment provides a salt-free extraction preparation process for heparin sodium, comprising the following steps:

[0054] S100, preparation of intestinal mucosa: take 20 small intestines of animals, pig small intestines are used in this example, crush the small intestines of animals into granules, mix them with water according to the ratio of small intestines to water of 1-12, add the mixture into a blender and stir In 10 minutes, the initial mucosal fluid was obtained;

[0055] S200, PH adjustment: adding an alkaline blending solution to the primary mucous fluid to raise the pH of the primary mucous fluid to 8.6;

[0056] S300, enzymatic hydrolysis of preliminary mucosal fluid: add compound enzyme solution to the primary mucosal fluid after pH adjustment to obtain mixed mucosal fluid, the ratio of compound enzyme solution to preliminary mucosal fluid is 1:30; and mix the mucosal fluid Add to enzymolysis equipment for enzymolysis;

[0057] S400, ultrasonic enzyma...

Embodiment 2

[0072] This embodiment provides a salt-free extraction preparation process for heparin sodium, comprising the following steps:

[0073] S100, preparation of intestinal mucosa: take 20 small intestines of animals, pig small intestines are used in this example, crush the small intestines of animals into granules, mix them with water according to the ratio of small intestines to water of 1-18, add the mixture into a blender and stir After 15 minutes, the initial mucosal fluid was obtained;

[0074] S200, PH adjustment: adding an alkaline blending solution to the primary mucous fluid to raise the pH of the primary mucous fluid to 9.4;

[0075] S300, enzymatic hydrolysis of preliminary mucosal fluid: add compound enzyme solution to the primary mucosal fluid after pH adjustment to obtain mixed mucosal fluid, the ratio of compound enzyme solution to preliminary mucosal fluid is 1:30; and mix the mucosal fluid Add to enzymolysis equipment for enzymolysis;

[0076] S400, ultrasonic e...

Embodiment 3

[0091] This embodiment provides a salt-free extraction preparation process for heparin sodium, comprising the following steps:

[0092] S100, preparation of intestinal mucosa: take 20 animal small intestines, pig small intestines are used in this example, crush the animal small intestines into granules, mix them with water according to the ratio of animal small intestines to water is 1-14, add the mixture into a blender and stir In 12 minutes, the initial mucosal fluid was obtained;

[0093] S200, PH adjustment: adding an alkaline blending solution to the primary mucous fluid to raise the pH of the primary mucous fluid to 8.8;

[0094] S300, enzymatic hydrolysis of preliminary mucosal fluid: add compound enzyme solution to the primary mucosal fluid after pH adjustment to obtain mixed mucosal fluid, the ratio of compound enzyme solution to preliminary mucosal fluid is 1:30; and mix the mucosal fluid Add to enzymolysis equipment for enzymolysis;

[0095] S400, ultrasonic enzym...

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Abstract

The invention discloses a heparin sodium salt-free extraction preparation process, which comprises the following steps of: intestinal mucosa preparation: crushing animal small intestines into particles, adding clear water for mixing according to the ratio of the animal small intestines to water being 1: (12-18), adding the mixture into a stirrer, and stirring for 10-15 minutes to obtain a primary mucosa liquid; and PH adjustment: adding an alkaline blending liquid into the primary mucosa liquid, and raising the PH of the primary mucosa liquid to 8.6-9.4. The method has the following beneficial effects that the pH value of the animal small intestines is adjusted, then a compound enzyme solution is added to carry out enzymolysis on the mucous membrane liquid to enable heparin sodium contained in the animal small intestines to be fully subjected to enzymolysis, and then ultrasonic enzymolysis and quaternary ammonium salt extraction are carried out to enable the extraction content of the heparin sodium to reach the highest. Compared with the prior art, the heparin sodium extracted by the process has the advantages that the content of the heparin sodium finally obtained from every 2000 animal small intestines reaches 1075 g, the yield is high, and the quality is stable.

Description

technical field [0001] The invention relates to the technical field of heparin sodium extraction, in particular to a preparation process for heparin sodium salt-free extraction. Background technique [0002] Heparin sodium (Heparin sodium) can interfere with many links in the blood coagulation process, and has anticoagulant effects both in vivo and in vitro. Its mechanism of action is relatively complex, mainly by combining with antithrombin III (AT-III), and enhancing the inhibitory effect of the latter on activated coagulation factors II, IX, X, XI and XII, and its consequences involve preventing platelet aggregation and Destroy and hinder the formation of thromboplastin, prevent prothrombin from turning into thrombin, inhibit thrombin, thereby preventing fibrinogen from turning into fibrin, thereby exerting an anticoagulant effect. [0003] The commonly used heparin sodium extraction method is mainly by salting-out method: the heparin sodium is separated from the complex...

Claims

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Application Information

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IPC IPC(8): C08B37/10
CPCC08B37/0003C08B37/0078
Inventor 徐袁陈讲宇余璞斐黄帅涂亚兰
Owner 潢川县鹏升畜产品有限公司
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