A kind of obtaining method of wild fungus of rare medicinal Anluo diphtheria
A technology of Anluo Xiaopi mushrooms and strains, which is applied in the field of obtaining wild strains of rare medicinal Anluo Xiaopi mushrooms, can solve problems such as difficulty in obtaining Anluo Xiaopi mushrooms, and achieve good application prospects and operation simple effect
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[0018] 1) Preparation of special medium:
[0019] The ingredients are: 200g potatoes, 10g glucose, 10g fructose, 5mL wort juice, 0.25g magnesium sulfate, 0.5g dipotassium hydrogen phosphate, 20g agar, vitamin B 1 100mg, distilled water 1000mL, pH7.5.
[0020] The preparation steps are as follows: boil the potatoes and filter them in water, then add in order to remove vitamin B 1 For other medicines, after being completely dissolved, dilute to 1000mL, divide into test tubes and sterilize. After sterilization, when the culture medium is cooled to below 50°C, add vitamin B in the ultra-clean workbench 1 , and then use precision pH test paper to adjust the pH of the solution to 7.5, and divide into test tubes for later use.
[0021] 2) Collect wild Anluo Xiaopi mushroom cords grown on a specific substrate: take Anluo Xiaopi which grows in the wild on the rotten wood of Chinese pine with an air temperature of 15-18°C and a humidity of about 90% and a length of 2-3cm umbrella (s...
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