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A kind of rhizobia and its application

A technology of rhizobia and microbial strains, applied in the field of microorganisms, can solve the problems of easily damaged soil structure and environmental pollution, and achieve the effect of promoting plant growth and increasing yield

Active Publication Date: 2021-06-04
XIAN UNVERSITY OF ARTS & SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Potassium and phosphorus in soil mostly exist in minerals such as aluminosilicate and apatite. Potassium and phosphorus in the form of mineral potassium and mineral phosphorus cannot be directly absorbed by plants. Chemical potassium and chemical phosphorus are commonly used in agriculture. The way of supplementing plants in form is easy to damage the soil structure and cause environmental pollution

Method used

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  • A kind of rhizobia and its application
  • A kind of rhizobia and its application

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Experimental program
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Embodiment Construction

[0036] Screening of strains:

[0037] Step 1. Enrichment: Collect about 20g of soil samples from Ziwei Garden, Baisha Road, Bowen Road Square, and Ganjiazhai next to the ground surface in Yanta District, Xi'an City, about 20g each, and put the collected soil samples into an oven to dry , pass the dried soil through a 100-mesh sieve, accurately weigh 1g of the soil that has passed through a 100-mesh sieve in a sterile manner, put it in 9mL of sterile water, let it stand for 30min, shake it at room temperature for 30min, and obtain a dilution of 10 -1 soil suspension; suck out 1mL from the above soil suspension, add 9mL sterile water, and mix thoroughly to obtain a dilution of 10 -2 soil suspension; from a dilution of 10 -2 Aspirate 1mL from the soil suspension, add 9mL sterile water, and mix well to obtain a dilution of 10 -3 soil suspension;

[0038] Step 2, preliminary screening and purification: take the dilution in step 1 as 10 -30.1mL of the soil suspension was spread ...

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Abstract

The invention discloses a rhizobium bacterium Rhizobium sp. WP 1‑3, and the preservation number is CGMCC No. 14472. In addition, the invention also discloses the application of the rhizobia in decomposing soil potassium and insoluble phosphorus in soil. The rhizobia of the present invention is a dominant bacterial strain screened out with potassium feldspar as a potassium source. During the process of growth and reproduction, the bacterial strain can convert the insoluble potassium element in the aluminosilicate in the soil into a soluble component. Decomposition of insoluble phosphorus to increase the source of phosphorus in the soil. In addition, the strain also has the function of nitrogen fixation, and provides nitrogen needed for plant growth and metabolism.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a rhizobia and application thereof. Background technique [0002] Potassium and phosphorus are important nutrients in plants, necessary for biological nitrogen fixation, and key components for promoting plant growth and metabolism. Potassium and phosphorus play an important role in regulating cell osmosis, material transport, promoting enzyme activation, promoting photosynthesis, increasing the assimilation rate of carbon dioxide, promoting the synthesis and transport of substances in crops, and enhancing plant resistance and tolerance. Potassium and phosphorus in soil mostly exist in minerals such as aluminosilicate and apatite. Potassium and phosphorus in the form of mineral potassium and mineral phosphorus cannot be directly absorbed by plants. Chemical potassium and chemical phosphorus are commonly used in agriculture. The way of supplementing the plants i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/41C09K101/00
CPCC09K17/14C09K2101/00C12N1/205C12R2001/41
Inventor 赵咏梅杨银银王华薛杨
Owner XIAN UNVERSITY OF ARTS & SCI
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