A method for the enrichment, extraction and identification of the mitochondrial genome of the river clam

A mitochondrial genome and identification method technology, applied in the field of molecular biology, can solve problems such as difficult to achieve, small total amount, low extraction purity, etc., and achieve the effect of large total amount, good discrimination, and good DNA integrity
CN109112190BActive Publication Date: 2021-09-17FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
Publication Date
2021-09-17

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Abstract

The invention provides a method for enriching and extracting the mitochondrial genome DNA of the clam and identifying the clam through the mitochondrial gene. Through the enrichment and extraction method provided by the present invention, a sufficient amount of mitochondrial genome DNA of the river clam can be successfully obtained. The present invention designs the primer pair Mit1 of the nad2-cox3 gene according to the mitochondrial sequence of the clam, and the primer pair Mit2 designed according to the cob gene fragment. The two pairs of primers provided by the present invention have strong specificity and can specifically amplify the mitochondrial sequence of the clam , but the gene fragment cannot be amplified for the mitochondria of other species. It has the advantages of good amplification performance, good discrimination, and less by-products. It can be used for species identification of river clams.
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Description

technical field

[0001] The invention belongs to the technical field of molecular biology, and relates to a method for enriching, extracting and identifying the mitochondrial genome of the clam, in particular to specifically identifying the mitochondria of the clam through a pair of specific primers. Background technique

[0002] River clams (Corbicula sp.), also known as clams, sand snails, snails, etc., belong to the clambranchia, euplobranchial order, clam family, clam genus. River clam is a bivalve aquatic organism with strong environmental adaptability, widely distributed in China, Korea, Japan and Southeast Asian countries. In my country's inland waters, river clams live in caves on the surface of the underwater soil and feed on plankton. They grow fast and have strong reproductive capabilities, making them suitable for artificial breeding. River clam meat is rich in nutrients and can be eaten fresh or used as medicine. It has the functions of appetizing, improving eye...

Claims

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