A method for constructing direct organogenesis regeneration pathway of Dangshansu pear

Abstract

The invention provides a method for constructing the direct organogenesis-type regeneration pathway of Dangshansu pear. The steps are as follows: (1) Acquisition of explants: two sources, one is tissue culture of Dangshansu pear with a leaf age of 20-50 days Seedling blade; another kind, utilize the pear seed aseptic sowing earlier, then adopt the blade of aseptic seedling as explant; Then place it in the pre-culture medium for dark culture; (3) Induction and proliferation of adventitious buds: inoculate the leaves on the induction medium for dark culture, then transfer to the photoperiod culture room to induce adventitious buds; cut off the adventitious buds, inoculate until multiplication Medium. The present invention establishes a genetically stable Dangshansu pear leaf regeneration system for the first time, the leaf regeneration rate reaches 78.6%, the average number of adventitious buds per leaf is 3.8, and the browning rate is 0; this method can not only directly produce a large number of adventitious buds from pear leaf organs, It also has the advantages of stable genetics, short cycle time, and low cost.

Description

technical field [0001] The invention relates to the technical field of plant cell engineering, in particular to a method for constructing a Dangshansu pear direct organogenesis regeneration pathway. Background technique [0002] Pear is a worldwide fruit tree species, belonging to Rosaceae, which is cultivated and distributed in 85 countries on five continents. Pear cultivation has a long history, at least 3,000 years old. As early as the pre-Qin period in China, there is a record in "The Book of Songs Qin Feng Chen Feng Pian": "There are buds in the mountains, and trees in the mountains." At present, there are 22 species widely recognized in the genus Pyrus, of which 13 species originate from China. Therefore, China is the country with the most abundant pear variety resources in the world. The main cultivated species of pears in my country include: white pear, sand pear, Qiuzi pear and Xinjiang pear, among which white pear and sand pear have the most extensive cultivation ...

AI Technical Summary

Problems solved by technology

Since then, scientists from various countries have done further research on the genetic transformation of pear, but compared with foreign research, China's research on this aspect is nearly 10 years later than foreign countries, and in the existing reports, pear Genetic transformation is mainly concentrated on Western pear varieties, and there are few reports on Oriental pears, especially the white pear variety Dangshansu pear belonging to Oriental pears.

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