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Anti-interference determination method for enzyme electrode biosensor

A technology of biosensor and measurement method, which is applied in the field of anti-interference measurement of enzyme electrode biosensors, can solve the problems of low measurement results and inability to reflect the actual concentration of the analyte, and achieve accurate measurement results

Active Publication Date: 2019-01-08
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

If there is such a reversible inhibitor in the measured substance of the enzyme electrode sensor, because the enzyme activity is inhibited during the measurement, the measurement result will be low and cannot reflect the actual concentration of the analyte

Method used

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  • Anti-interference determination method for enzyme electrode biosensor
  • Anti-interference determination method for enzyme electrode biosensor
  • Anti-interference determination method for enzyme electrode biosensor

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Embodiment Construction

[0034] The present invention will be further described below.

[0035] An enzyme electrode biosensor anti-interference assay method, comprising the steps of:

[0036] In the case that there is an enzyme activity inhibitor in the determination substrate, the calibration of the enzyme activity is also carried out in the same environment, so that the calibration and determination status are in the same enzyme reaction environment. The influence of enzyme activity inhibitors on the determination process was excluded. In the measurement process, the sample to be tested is directly injected according to the set injection volume (nμl), and the response current value of the enzyme electrode sensor to the sample is measured, and repeated several times until the response current value of the sensor is basically unchanged (less than the set error value), record the average value X of the response current measured for the last two times (or the last response current value, taking the las...

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Abstract

An anti-interference determination method for an enzyme electrode biosensor comprises the following steps: constant a and Michaelis constant b are calculated by firstly measuring corresponding currentvalue X of a to-be-tested sample, then measuring corresponding current value Y of the to-be-tested sample and equivalent standard sample and finally measuring corresponding current value Z of the to-be-tested sample and half of the standard sample. Concentration of the to-be-tested sample after compensation is calculated finally, influence of interference factors is eliminated in the determination method, and a determination result obtained with the determination method is more accurate and more reliable under the condition of presence of an interference source.

Description

technical field [0001] The invention relates to the field of enzyme electrode biosensors, in particular to an anti-interference measuring method for enzyme electrode biosensors. Background technique [0002] Enzyme electrode biosensor is a composite sensor that combines biologically active enzymes with electrochemical electrodes. The response current of the electrochemical electrode finally obtains the concentration data of the enzyme reaction substrate. [0003] An enzyme is a protein whose catalytic activity gradually inactivates over time. Therefore, the enzyme electrode sensor is not a permanent sensor and has a certain service life. The life of the enzyme electrode sensor is related to the type of the enzyme electrode sensor, usually several days to several months. Although the catalytic activity of the enzyme electrode sensor changes with time, the change in activity in a short period of time (minutes to tens of minutes) is negligible. The application of enzyme elec...

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 朱思荣毕春元高广恒张利群赵晓华张金玲杜祎
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI