PCR laboratory with environmental barrier

A laboratory and barrier technology, applied in the field of PCR laboratory, can solve problems such as incorrect direction of gas flow pressure gradient, false positive test results, contaminated reagents and samples, etc., to achieve true test results, control positive false positives, and prevent gas mutual penetration leaky effect

Pending Publication Date: 2019-01-11
ZHONGCHUAN NO 9 DESIGN & RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the anti-cross-contamination control methods in each test area of ​​the PCR laboratory are varied, chaotic, and full of mistakes
Neither the positive pressure area nor the negative pressure area of ​​the laboratory air flow pressure gradient is scientifically and reasonably divided, and the dedicated corridor is not separated, and the direction of the air flow pressure gradient is not correct. Reliable air flow blocking cannot be achieved, which may easily lead to the amplification / product analysis area. The aerosol containing nucleic acid diffuses out, contaminating reagents and samples, causing false positives in experimental results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Such as Figure 1-3 The shown PCR laboratory with environmental barriers includes a reagent preparation room 1, a sample preparation room 2, an amplification room 3 and a product analysis room 4 arranged sequentially from left to right, wherein:

[0060] A first transfer window 12 is provided on the wall between the reagent preparation chamber 1 and the sample preparation chamber 2, and a second transfer window 23 is provided on the wall between the sample preparation chamber 2 and the amplification chamber 3. A third transfer window 34 is provided on the wall between the amplification chamber 3 and the product analysis chamber 4;

[0061]A first buffer chamber 5 is provided on the front side of the reagent preparation chamber 1, a second buffer chamber 6 is provided on the front side of the sample preparation chamber 2, and a third buffer chamber 6 is provided on the front side of the amplification chamber 3. buffer room 7;

[0062] On the front side of the first buf...

Embodiment 2

[0078] A PCR laboratory with an environmental barrier, the rest are the same as in Example 1, the air pressure P1 in the reagent preparation chamber 1=the air pressure P5+5Pa in the first buffer chamber 5;

[0079] Air pressure P5+5Pa in the first buffer chamber 5=air pressure P8+5Pa in the dedicated corridor 8 in the positive pressure zone;

[0080] Air pressure P8 in the special corridor 8 of the positive pressure area = outdoor normal temperature pressure P 0 +5Pa;

[0081] Air pressure P9 in the dedicated corridor 9 in the negative pressure area = outdoor normal temperature pressure P 0 -5Pa;

[0082] Air pressure P7 in the third buffer chamber 7 = air pressure P9-5Pa in the dedicated corridor 9 in the negative pressure zone;

[0083] Air pressure P3 in the amplification chamber 3 = air pressure P7-5Pa in the third buffer chamber 7 .

Embodiment 3

[0085] A kind of PCR laboratory with environmental barrier, all the other are the same as embodiment 2, and described reagent preparation room entrance and exit door 15 is offered toward the direction of reagent preparation room 1;

[0086] The entrance and exit door 26 of the sample preparation chamber is opened toward the direction of the sample preparation chamber 2;

[0087] The entrance and exit door 37 of the amplification chamber is opened towards the direction of the third buffer chamber 7;

[0088] The entrance and exit door 49 of the product analysis room is opened in the direction of the dedicated corridor 9 in the negative pressure area;

[0089] The first buffer door 58 is opened towards the direction of the first buffer chamber 5;

[0090] The second buffer door 68 is opened towards the direction of the second buffer chamber 6;

[0091] The third buffer door 79 is opened in the direction of the dedicated corridor 9 in the negative pressure area;

[0092] The p...

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Abstract

The invention relates to the technical field of PCR laboratories, in particular to a PCR laboratory with environmental barrier. The PCR laboratory with environmental barrier comprises a reagent preparation room, a sample preparation room, a proliferation room and a product analysis room arranged sequentially from left to right, wherein the wall body between the reagent preparation room and the sample preparation room is provided with a first transfer window; the wall body between the sample preparation room and the proliferation room is provided with a second transfer window; and the wall bodybetween the proliferation room and the product analysis room is provided with a third transfer window. The PCR laboratory with environmental barrier has the beneficial effects that the laboratory isan innovative achievement through many years of practice, research and summary; and the technology of the invention is mainly embodied in the following aspects: structural divisions are laid firstly in a scientific and reasonable technology; setting in a scientific and reasonable airflow pressure gradient direction is firstly carried out; a reasonable gate opening direction of each functional roomis firstly carried out; and a personnel flow and sample flow direction is firstly laid in a reasonable mode.

Description

technical field [0001] The invention relates to the technical field of PCR laboratories, in particular to a PCR laboratory with an environmental barrier. Background technique [0002] PCR experiment refers to gene amplification experiment. PCR laboratory is widely used in various fields of biology, such as: AIDS detection, hepatitis B, avian epidemic disease, cancer gene detection and diagnosis, DNA fingerprint, individual identification, paternity identification and forensic medicine Physical evidence, animal and plant quarantine, animal and derivative product testing, animal feed, cosmetics, food hygiene testing, genetically modified crops and genetically modified microorganisms, etc. [0003] Generally, PCR laboratories can be divided into reagent preparation rooms, sample preparation rooms, amplification, product analysis and other rooms according to the methods used. For laboratories that only use automatic amplification detectors (such as fluorescent quantitative PCR ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E04H1/12E04B2/00
CPCE04H1/12E04B2/00
Inventor 陈文云
Owner ZHONGCHUAN NO 9 DESIGN & RES INST
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