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A dual rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease virus and preparation method thereof

A foot-and-mouth disease virus and detection card technology, applied in measuring devices, analytical materials, instruments, etc., can solve problems such as antigen rapid detection cards that have not yet been seen, and achieve intuitive results display, high detection efficiency, high specificity and sensitivity. Effect

Active Publication Date: 2021-10-01
ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, detection of 3ABC protein antibody can be used as an important indicator for diagnosing wild virus infection. Most of the FMDV colloidal gold rapid detection cards that appear at present are based on test strips for detecting 3ABC protein antibody, such as patents CN105954514A, CN202814986U, CN101236204, CN1798976B, etc. It belongs to the colloidal gold rapid test card for detecting FMDV antibodies; while most of the FMDV antigen rapid test cards that have appeared so far are for the detection of O-type FMDV, there is no antigen rapid test card that can detect O-type and A-type FMDV at the same time

Method used

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  • A dual rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease virus and preparation method thereof
  • A dual rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease virus and preparation method thereof
  • A dual rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease virus and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0065] like Figure 1-2 As shown, one detection and distinguishing between the O-type, the two-stage fast detection card of the A-type open-and-mouth disease virus, including the casings and test strip, the test strip including the support plate 1 and the adsorption layer fixed to the support plate, the adsorption layer from the test The end starts sequentially for the sample pad 3, the goldbat pad, the detection film 2 and the water absorbing pad 9, the gold bond pad includes the upper and lower laminated pads 4 and the lower goldbatch pad 5 and the lower gold bond pad 5 on the support plate. The anti-O-mouth-made anti-O-flavor monoclonal antibody MABIII was adsorbed on the lower gold-labeled anti-A-type mouth-shoe virus monoclonal antibody MABIV, and the film anti-mouse anti-mouse was attached to the lower gold-labeled anti-A-type mouth-shaped virus monoclonal antibody MABIV. IgG antibody or golden Staphylococcus Sapulus Steit Sapa Steit Sapa Steit Channel C 6, anti-O-FMDV monocl...

Embodiment 2

[0070] Preparation of the double fast detection card of the O-FMDV and A-FMDV of the present invention: First, an O-FMDV immune antigen and a A-FMDV immune antigen, which are prepared to prepare anti-O-FMDV monoclonal antibody and A-FMDV monoclonal antibody, and Filter monoclonal antibodies identifying O-FMDV and A-FMDV. Among them, O-FMDV monoclonal antibody MABIII and A-FMDV monoclonal antibody MABIV is used to prepare a gold standard antibody, a monoclonal antibody MABI, which is different from MABIII identifying an antigenic epitope for printing O-FMDV detection. Line blot T1 "│", specific identification A-FMDV and monoclonal antibody MABII different from MABIV recognition antigenic epitope is used to print the A-FMDV detection line blot T2 "│"; secondly prepare lamb anti-mouse anti-mice IgG antibody or golden Staphylococcus SPA for printing quality control line blotting C "│"; re-assembling prepared sample pad, goldbatch pad, detection film and water absorbing pad, and suppor...

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Abstract

The invention relates to a double-link rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease viruses and a preparation method thereof. The detection card uses the principle of double-antibody sandwich ELISA to detect antigens. The monoclonal antibody mAbI that can specifically recognize the O-type foot-and-mouth disease virus and the monoclonal antibody mAbII that can specifically recognize the A-type foot-and-mouth disease virus are sprayed onto the detection membrane (nitrocellulose membrane) ) at the T1 and T2 detection line positions to prepare a detection imprint; spray goat or rabbit anti-mouse IgG or SPA to the C line position of the quality control area of ​​the detection membrane to prepare a quality control imprint. The gold-labeled antibody fiber layer is divided into upper and lower layers, which are respectively adsorbed with colloidal gold-labeled monoclonal antibody mAbIII that can specifically recognize type O foot-and-mouth disease virus and monoclonal antibody mAbIV that can specifically recognize type A foot-and-mouth disease virus. The test strip can be used for rapid detection of O-type and A-type foot-and-mouth disease virus (FMDV) infection. By applying the invention, the operation is simple and fast, the result is clear and easy to argue, and is suitable for popularization at the grassroots level.

Description

Technical field [0001] The present invention relates to the simultaneous detection of the two Veterinary instrument identification, detection, and more particularly to a type O, cards and rapid detection method for preparing double-mouth disease virus type A distinction. Background technique [0002] An acute FMD (foot-and-mouth disease, FMD) by FMDV (foot and mouthdisease virus, FMDV) causes cloven-hoofed animals (pigs, cattle, sheep), the heat resistance, highly contagious disease susceptible Up to 70 species of animals, the disease has infected more than animal species, spread fast, highly contagious, causing huge economic losses and so on. FMD is "OIE disease, infection and infestation List" in Reportable 25 kinds of animals were suffering from one of a variety of infectious and parasitic diseases, the OIE as Class A infectious diseases, my country's Ministry of Agriculture requires mandatory immune prevention and control of animal diseases. [0003] FMDV belongs to the small...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/531
CPCG01N33/531G01N33/54306G01N33/56983
Inventor 张改平刘运超王爱萍刘东民赵建国陈玉梅石海宁周景明王方雨王彦伟
Owner ZHENGZHOU UNIV
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