37results about How to "Combination does not affect" patented technology

The invention discloses a paper-based micro-fluidic chip enhancement type chemiluminescence hlyA gene detection method. The method comprises the steps that (1) a screen printing board is pressed on chromatography paper, coated with wax and heated, and the chromatography paper is naturally disengaged from the screen printing board and aired; (2) a catching probe and a signal probe are designed; (3) a paper-based micro-fluidic chip is pretreated; (4) a DNA sample to be tested and the catching probe are incubated, the signal probe is added to conduct a hybridization reaction with the DNA sample to be tested, and HRP-SA is added for incubation; finally a base solution is added to trigger enhancement type chemiluminescence, and an luminescence signal is collected by a CCD digital imaging device in an imaging mode. Compared with an expensive and complex optical imaging system, the simple CCD device is adopted for imaging detection, an enhancement type chemiluminescence system is combined with a biotin-streptavidin affine magnification system, the detection flexibility of paper-based micro-fluidic chip enhancement type chemiluminescence is greatly improved, and the detection limit can reach 6.3*10<-2>pmol / L.

The invention discloses a test strip that is used for detecting antibodies of porcine parvovirus and porcine encephalitis B virus in one step, which comprises a supporting layer, an adsorptive layer and a protecting layer, and the adsorptive layer consists of a sample fiber layer, an adsorptive gold mark fiber layer, a fibrin membranous layer and a water absorptive material layer sequentially from a testing end; the adsorptive gold mark fiber layer adsorbs any one or two from antigen liquids of gold colloid marked SPA or gold colloid marked pure PPV and JEV; a detecting print and a contrast print are arranged on the fibrin membranous layer, the detecting print is printed with any one or two from the purified PPV and JEV antigen liquids, and the contrast print is printed with an IgG solution of ovine or rabbit anti-SPA or an IgG solution of any one or two viruses from ovine or rabbit anti-PPV and anti-JEV. The test strip of the invention has strong detecting specificity and high sensitivity, avoids additional equipment and reagents, can be operated by every person, and is particularly applicable to the quick specific antibody detection of porcine diseases on the spot.

The invention discloses a liquid biochip system for binding, separating and purifying a targeting biomacromolecule. The system comprises a liquid chip consisting of a micron or nanometer chip particle and chip particle storage buffer solution, and liquid chip work buffer solution, wherein the liquid chip work buffer solution comprises sample modifying buffer solution, binding buffer solution, washing buffer solution, eluting buffer solution, and / or chip passivating buffer solution, and / or chip activating buffer solution. The system has the advantages of good repeatability, high sample processing throughput, simple operation, low cost and the like, has extremely wide application field, and is applicable to separation and purification of various biomacromolecules.

The invention provides an EB virus VCA-IgA antibody detection reagent which at least comprises a reactive film, an antigen pad and a gold-labeled pad, wherein a detection line and a quality control line are marked on the reactive film; the detection line contains a mouse anti-human IgA monoclonal antibody; the quality control line contains biotin; the antigen pad contains a biotin-labeled recombinant EB virus VCA antigen; and the gold-labeled pad contains an avidin complex labeled by colloidal gold. The detection kit disclosed by the invention has the characteristics of rapidness, simplicity, accuracy and high accuracy.

The present invention relates to a test strip which is used for testing a kind of or a plurality of kinds of disease antibodies of porcine virus diarrhea; the test strip comprises a supporting layer and a reaction reagent carrier absorbing layer which is pasted on the supporting layer; the reaction reagent carrier absorbing layer comprises a testing end fiber layer, a fiber layer of absorbing gold-labeled SPA protein or gold-labeled antigen which corresponds to the antigen to be tested, and a cellulose layer, which are arranged at the sample end in sequence, and an absorbent material layer which is positioned at the handle end; the cellulose layer contains one, two or three testing print(s) which are printed by anyone, any two or three of the purified transmissible gastroenteritis virus TGEV solution, the porcine epidemic diarrhea virus PDEV solution and the porcine rotavirus RV, and the cellulose layer also contains the contrast prints which are printed by anyone, any two or three of the anti-SPA protein IgG solution of the sheep or the rabbit or the anti-TGEV, anti-PDEV, anti-RV IgG solution of the sheep or the rabbit; and the invention provides a test strip for testing a kind of or a plurality of kinds of disease antibodies of the porcine virus diarrhea, which has the advantages of accurate and rapid detection, convenient operation and low costs.

The invention discloses a magnetic-sensitive rubber composition for a rubber air spring, and a preparation method thereof. The rubber composition comprises, by weight, 100 parts of matrix rubber, 2-8parts of an activator, 0-50 parts of carbon black, 0-20 parts of white carbon black, 0-4 parts of a silane coupling agent, 1-3 parts of an accelerant, 3-6 parts of an anti-aging agent, 1-2 parts of avulcanizing agent, 200-600 parts of modified ferromagnetic particles and 1-10 parts of a self-repairing agent, wherein the matrix rubber is composed of natural rubber or chloroprene rubber or hydrogenated butadiene-acrylonitrile rubber; the activator is composed of nanometer zinc oxide and stearic acid; the white carbon black is high-dispersion precipitated white carbon black; the silane couplingagent is a liquid silane coupling agent Si-69; the accelerant is an accelerant CBS; the anti-aging agent is composed of an anti-aging agent 6PPD and an anti-aging agent RD; the vulcanizing agent is sulfur; and the self-repairing agent is liquid nitrile rubber-phenolic resin. The tensile strength of the magnetic-sensitive rubber composition reaches 15 MPa or above, and the elongation at break is 400% or above.

The invention discloses a module type hydraulic classification device with a variable cone angle. The module type hydraulic classification device with the variable cone angle is characterized in that the module type hydraulic classification device with the variable cone angle comprises three modules; the first module is composed of an upper shell body, an overflowing collecting groove and a feeding well, the upper shell body is located on the upper portion and of a conical structure, the overflowing collecting groove is formed around the outer conical surface located below a feeding port of the upper shell body, and the feeding well is arranged in the feeding port of the upper shell body in a penetrating mode through a fixing piece in the mode that the feeding well and a hydraulic classification machine body are coaxial; the second module is composed of a lower shell body, the lower shell body is located in the middle and buckled with the upper shell body, the cone angle of the lower shell body is variable, and the lower shell body is of a reverse-cone structure; the third module is composed of a hollow column section and a conical discharging section, the hollow column section is installed in the position of a discharging port formed in the bottom of the lower shell body and provided with a top water inlet pipe opening, and the conical discharging section is arranged at the bottom of the hollow column section; the upper end of the feeding well is higher than an upper edge opening of the overflowing collection groove, and the lower end of the feeding well is located above the connection portion between the second module and the third module.

The present invention relates to a magnetic resonance molecular probe for detecting early-stage hepatocellular carcinoma. The magnetic resonance molecular probe comprises: the ligand of a glypican-3 (GPC3) receptor, wherein the ligand is a biotinylated polypeptide having an amino acid sequence represented by SEQ ID NO:1; and a MR imaging agent, wherein the imaging agent is a streptavidin-polyethylene glycol-ultra-small superparamagnetic iron oxide (SA-PEG-USPIO) nanometer compound, and the biotinylated polypeptide and the SA-PEG-USPIO are combined through biotin-avidin to form the GPC3-targeting magnetic resonance molecular probe. According to the present invention, the magnetic resonance molecular probe can be combined with the specific receptor expressed in hepatocellular carcinoma cells, does not have immunogenicity and toxicity or has low immunogenicity and low toxicity, and can improve the signal-to-noise ratio of the image of the MR imaging.

The invention provides an anti-OX40 antibody. Furthermore, the invention provides a nucleic acid sequence encoding the antibody, a vector comprising the nucleic acid sequence, and host cells transformed or transfected with the vector. Further, the invention provides pharmaceutical use of the antibody.

The invention relates to a double rapid test strip for detecting and identifying type O and A foot and mouth disease viruses and a preparation method thereof. According to the test strip, on the basisof a principle of detecting antigens through double antibody sandwich ELISA, monoclonal antibodies mAbI capable of specifically identifying a type O foot and mouth disease virus and monoclonal antibodies mAbII capable of specifically identifying a type A foot and mouth disease virus are sprayed at positions of T1 and T2 detection lines of detection membrane (nitrocellulose membrane), so detectionimprints are prepared; and goat or rabbit anti-mouse IgG or SPA is sprayed at the position of a C line in a quality control area, so quality control imprints are prepared. Gold labeled antibody fibrous layers comprise an upper layer and a lower layer and are respectively used for absorbing colloidal gold labeled monoclonal antibodies mAbIII capable of specifically identifying the type O foot andmouth disease virus and colloidal gold labeled monoclonal antibodies mAbIV capable of specifically identifying the type A foot and mouth disease virus. The test strip can be used for rapidly detectingtype O and A foot and mouth disease virus (FMDV) infection. Through application of the test strip and the preparation method, operation is simple and rapid, a result is clean and is easy to identify,and the test strip and the preparation method are applicable to basic level popularization.

The invention provides an EB (epstein-barr) virus VCA / NA1-IgA antibody joint detection reagent. The EB virus VCA / NA1-IgA antibody joint detection reagent at least comprises a reaction membrane, an antigen pad and a gold label pad, wherein a detection line and a quality control line are labeled on the reaction membrane; the detection line contains a rat anti-human IgA monoclonal antibody; the quality control line contains biotin; the antigen pad contains a recombinant EB virus VCA antigen labeled with the biotin and a recombinant EB virus NA1 antigen labeled with the biotin; and the gold label pad contains an avidin compound containing a colloid gold label. The joint detection reagent provided by the invention is used for carrying out joint detection on VCA and NA1 so that the cost can be saved; the application and the popularization of the detection reagent are facilitated and the detection cost is reduced; and the EB virus VCA / NA1-IgA antibody joint detection reagent has the characteristics of rapidness, simplicity and convenience, accuracy and high sensitivity.

The invention discloses a magnetic sensitive rubber composition used for rubber air springs and a preparation method thereof. The components include 100 parts of base rubber, 2 to 8 parts of active agent, and 0 to 50 parts of carbon black in terms of weight ratio. , 0-20 parts of white carbon black, 0-4 parts of silane coupling agent, 1-3 parts of accelerator, 3-6 parts of anti-aging agent, 1-2 parts of vulcanizing agent, 200-600 parts of modified ferromagnetic particles, Restorative agent 1 to 10 parts. Among them, the matrix rubber is composed of natural rubber or chloroprene rubber or hydrogenated nitrile rubber; the active agent is composed of nano-zinc oxide and stearic acid; the white carbon black is highly dispersed precipitated silica; the silane coupling agent is liquid silane Coupling agent Si-69; accelerator is accelerator CBS; anti-aging agent is anti-aging agent 6PPD and anti-aging agent RD; vulcanizing agent is sulfur; self-healing agent is liquid nitrile rubber-phenolic resin. The magnetic sensitive rubber composition of the present application has a tensile strength of over 15 MPa and an elongation at break of over 400%.

The invention provides an EB (epstein-barr) virus NA1-IgA antibody detection reagent. The EB virus NA1-IgA antibody detection reagent at least comprises a reaction membrane, an antigen pad and a gold label pad, wherein a detection line and a quality control line are labeled on the reaction membrane; the detection line contains a rat anti-human IgA monoclonal antibody; the quality control line contains biotin; the antigen pad contains a recombinant EB virus NA1 antigen labeled with the biotin; and the gold label pad contains an avidin compound containing a colloid gold label. The detection reagent provided by the invention has the characteristics of rapidness, simplicity and convenience, accuracy and high sensitivity.

The invention relates to a test strip for rapidly identifying a foot-and-mouth-disease virus infected and vaccine immunized animal and a preparation method of the test strip. The test strip comprises a supporting layer and an adsorbing layer; the adsorbing layer is attached to the supporting layer; the adsorbing layer comprises a sample adsorbing fiber layer, a gold-labeled antibody fiber layer and a cellulose membrane layer sequentially arranged on the testing end and further comprises a water absorbing material layer arranged on the handle end; a detection blot and a control blot are arranged on the cellulose membrane layer; the colloidal gold labeled staphylococcus protein A is adsorbed on the gold-labeled antibody fiber layer; the detection blot is printed by one or two of a pig foot-and-mouth-disease virus protein structural VPI antigen and a non-structural protein 3ABC antigen which are expressed and purified by an escherichia coli expression system; and the control blot is printed by using a goat anti- or rabbit anti-SPA IgG antibody solution. The test strip is high in specificity, high in sensitivity, simple, direct, exact, wide in application scope, low in cost, capable of being used by professionals or non-professionals for detection at any time and any place and beneficial to popularization and application.

The invention discloses a preparation method for preparing a microcosmic porous structure material through freeze curing 3D printing, and relates to the technical field of 3D printing, the method comprises the following steps: preparing a support material, and placing the prepared support material in a mold; preparing the target material into target slurry for extrusion type printing; a mold containing a supporting material is placed on a printing platform, and the target slurry is placed in a charging barrel of a printing device; according to a target design model, the target slurry is extruded from the supporting material through a spray head of a printing device, and a printing model is obtained; freezing and solidifying the printing model to obtain a target model; and post-processing the target model to obtain the microscopic porous structure material. According to the preparation method, 3D printing and freeze curing forming are combined, and the microcosmic porous structure materials with different pore diameters and porosities are prepared by adjusting the component composition and the component concentration of the target slurry.

The invention discloses a fungaltoxin magnetochemiluminescence immunoassay kit based on bifunctional fusion protein and application of the fungaltoxin magnetochemiluminescence immunoassay kit. The kit comprises streptavidin magnetic particles, a biotin-labeled mycotoxin antigen, a mycotoxin standard substance solution, a mycotoxin nano antibody-alkaline phosphatase bifunctional fusion protein, a sample diluent, a scrubbing solution and a substrate solution. According to the kit, chemiluminescence, magnetic particle separation and genetic engineering technologies are gathered together, rapid, accurate and full-automatic detection of mycotoxin can be achieved, edible oil can be directly subjected to sample adding detection, extraction, centrifugation and other operations are not needed, the operation error of detection personnel is reduced, the requirement for the ability of the detection personnel is lowered, and the detection efficiency is improved. The detection efficiency of a large batch of samples at a basic level is remarkably improved, and a powerful means is provided for mycotoxin detection and monitoring.

The invention discloses an interleukin 2 binding molecule, which can specifically bind to interleukin 2 and comprises at least one immunoglobulin single variable domain; the at least one immunoglobulin single variable domain comprises CDR1, CDR2 and CDR3, wherein CDR1 comprises an amino acid sequence that is at least 90% identical to any one of SEQ ID NO: 1-15; CDR2 comprises an amino acid sequence that is at least 90% identical to any one of SEQ ID NO: 16-54; and CDR3 comprises an amino acid sequence that is at least 90% identical to any one of SEQ ID NO: 55-84. The invention also discloses a nucleic acid molecule encoded by the interleukin 2 binding molecule, an expression vector, a host cell, a production method, an immunoconjugate using the interleukin 2 binding molecule, a pharmaceutical composition, a kit and application of the interleukin 2 binding molecule. According to the invention, the interleukin 2 binding molecule with high specificity, high affinity and high stability is obtained through screening and is used for treating, preventing and diagnosing IL2 related diseases.

The invention relates to integrated self-amplification indirect competitive immunochromatography test paper. The test paper comprises a supporting layer, an adsorption layer and a protective layer, wherein the adsorption layer and the protective layer are fixed on the supporting layer; the adsorption layer sequentially comprises a sample pad, a conjugate pad I, a conjugate pad II, a cellulose membrane layer and a water absorption material layer which is located at a handle end, wherein the sample pad, the conjugate pad I, the conjugate pad II and the cellulose membrane layer are sequentially distributed from a test end; a detection line and a quality control line are sequentially arranged on the cellulose membrane layer; and the conjugate pad I adsorbs a target monoclonal antibody, and the conjugate pad II adsorbs an artificial antigen marked by a nanometer material and biotin marked by the nanometer material. According to the test paper, the antibodies do not need to be marked, protective liquid is added and dried on the conjugate pad I, folding and damage of the antibodies in the antibody marking process are avoided, and the antibody dosage can be accurately controlled; and the nano-particle labeled artificial antigen is used as a probe, and each antibody intercepted on the detection line can be accurately combined with two labeled antigens, so that signal self-amplification is realized, and the sensitivity of the immunochromatography test paper is higher.

The invention relates to a 'self-amplification' and 'precise control' indirect competitive immunochromatography detection test strip which consists of two parts, namely test paper and a sample cup. The test paper comprises a supporting layer, an adsorption layer fixed on the supporting layer and a protective layer. The adsorption layer sequentially comprises a sample pad, a combination pad, a cellulose membrane layer and a water absorption material layer at a handle end from a test end. A detection line and a quality control line are sequentially arranged on the cellulose membrane layer. The conjugate pad is made of glass fiber cotton adsorbed with a nano material labeled antigen. The sample cup is a container for diluting a sample, and a target monoclonal antibody is pre-fixed in the sample cup. According to the test strip, the antibody is independently dried in the sample cup, so that folding and damage in the antibody labeling process are avoided, and the antibody dosage can be accurately controlled; and nano-particle labeled artificial antigens are used as a probe, and each antibody intercepted on a detection line can be accurately combined with two labeled antigens, so that signal self-amplification is realized, and the sensitivity of the test paper is improved.

The invention relates to an action mechanism of a light fuel weapon, which is characterized of briefness. The principle is that two objects two inclined surfaces (under the condition of opposite moving direction) of which are intersectant can be adhered under a certain pressure and contact area, and the two objects can be in relative displacement with the reducing of the pressure. The subject of the invention is to solve the problem of the existing action in sealing and being simple and convenient. The action mechanism of a light fuel weapon opens and closes locks in a spiral mode.

The present invention discloses one kind of granular marker for biological detection, and the granular marker is granular matter with at least one marking component and at least one marked component on the surface. The granular marker is prepared with granular matter as carrier and through adsorbing, combining or connecting the marking component and the marked component directly or indirectly. The present invention has simple granular marker preparing process and doubled detected signal of the marker, and makes it possible to connect several kinds of marking components and marked components onto the same kind of granular matter for multiple marking.

The invention relates to a cast-in type burr cylinder sleeve and a manufacturing method of the cast-in type burr cylinder sleeve. Mushroom-shaped burrs are arranged on the outer portion of a cylindersleeve body, after follow-up cylinder block molten aluminum casting, combination performance between the cast-in type burr cylinder sleeve and an engine is significantly improved, the cast-in type burr cylinder sleeve is stripped with strong external force, outer circle protrusions of the cast-in type burr cylinder sleeve still remain in the cylinder block, and the heat conducting performance of the cast-in type burr cylinder sleeve is significantly improved. During casting, a specially-prepared mold release agent is adopted, the burrs are not prone to oxidation, the follow-up combination of the cast-in type burr cylinder sleeve and the engine is not influenced, and the yield of the cast-in type burr cylinder sleeve can reach 94%.

The invention discloses a vertically-opening ice breaking high-voltage isolation switch. The upper portion of an operation pull bar (11) is in hinged connection with one end of an ice breaking connecting rod (10). The other end of the ice breaking connecting rod (10) is in hinged connection with an ice breaking claw (7). The ice breaking claw (7) which can slide longitudinally along the surface of a conductive arm moving contact is arranged on the inner side of a moving contact area of a conductive arm (12). The tail portion of the ice breaking claw (7) is in hinged connection with the ice breaking connecting rod (10). The other end of the ice breaking connecting rod (10) is in hinged connection with the operation pull bar (11). According to the invention, through utilization of characteristics of relatively low shearing intensity of ice, motions of the moving contact pull bar simultaneously drives the motions of an ice breaking mechanism so as to realize ice breaking of the moving contact in a closing process; then the moving contact which is without ice carries out ice breaking on the static contact under working of the operation mechanism. The structure is simple and reasonable; the working is reliable; and requirements of rapid ice breaking can be satisfied.

The invention discloses a test strip that is used for detecting antibodies of porcine parvovirus and porcine encephalitis B virus in one step, which comprises a supporting layer, an adsorptive layer and a protecting layer, and the adsorptive layer consists of a sample fiber layer, an adsorptive gold mark fiber layer, a fibrin membranous layer and a water absorptive material layer sequentially from a testing end; the adsorptive gold mark fiber layer adsorbs any one or two from antigen liquids of gold colloid marked SPA or gold colloid marked pure PPV and JEV; a detecting print and a contrast print are arranged on the fibrin membranous layer, the detecting print is printed with any one or two from the purified PPV and JEV antigen liquids, and the contrast print is printed with an IgG solution of ovine or rabbit anti-SPA or an IgG solution of any one or two viruses from ovine or rabbit anti-PPV and anti-JEV. The test strip of the invention has strong detecting specificity and high sensitivity, avoids additional equipment and reagents, can be operated by every person, and is particularly applicable to the quick specific antibody detection of porcine diseases on the spot.

The invention relates to a magnetic resonance molecular probe for detecting early hepatocellular carcinoma. The magnetic resonance molecular probe for detecting the early hepatocellular carcinoma comprises: a ligand of a phosphatidylinositol proteoglycan 3 (GPC3) receptor, wherein the ligand is a biotinylated polypeptide (BT-L5) with an amino acid sequence as shown in SEQ ID NO: 1; an MR imaging developer, wherein the developer is a biotin polyethylene glycol ultra-small superparamagnetic iron oxide (BT-PEG-USPIO) nano composite, wherein the biotinylated polypeptide BT-L5 and the BT-PEG-USPIOare bridged through biotin-avidin to form the magnetic resonance molecular probe of the targeted GPC3. The magnetic resonance molecular probe not only can be combined with a specific receptor expressed by liver cancer cells in a targeting manner, but also has no immunogenicity (or is very low), no toxicity or low toxicity, and can provide enough image signal-to-noise ratio for MR imaging.

The invention discloses a spiral burr type aluminum alloy cylinder liner. The spiral burr type aluminum alloy cylinder liner comprises a cylinder liner body, the internal surface of the cylinder linerbody is coated with an internal coating, the surface roughness of the internal coating is 80-120 [mu]m, and the thickness of the internal coating is 0.2-0.4 mm; and the external surface of the cylinder liner body is provided with burrs, the burrs are spirally distributed, and the height of the burrs is 0.1-0.5 mm. The invention also discloses a method for producing the spiral bur type aluminum alloy cylinder liner. The spiral bur type aluminum alloy cylinder liner has the advantages of good biting force, long service life and good wear resistance.

The invention relates to a double-link rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease viruses and a preparation method thereof. The detection card uses the principle of double-antibody sandwich ELISA to detect antigens. The monoclonal antibody mAbI that can specifically recognize the O-type foot-and-mouth disease virus and the monoclonal antibody mAbII that can specifically recognize the A-type foot-and-mouth disease virus are sprayed onto the detection membrane (nitrocellulose membrane) ) at the T1 and T2 detection line positions to prepare a detection imprint; spray goat or rabbit anti-mouse IgG or SPA to the C line position of the quality control area of ​​the detection membrane to prepare a quality control imprint. The gold-labeled antibody fiber layer is divided into upper and lower layers, which are respectively adsorbed with colloidal gold-labeled monoclonal antibody mAbIII that can specifically recognize type O foot-and-mouth disease virus and monoclonal antibody mAbIV that can specifically recognize type A foot-and-mouth disease virus. The test strip can be used for rapid detection of O-type and A-type foot-and-mouth disease virus (FMDV) infection. By applying the invention, the operation is simple and fast, the result is clear and easy to argue, and is suitable for popularization at the grassroots level.

The invention discloses a method for preparing a gradient copper matrix composite material and for integrating a part. The method comprises the steps of preparing reaction flakes with different proportions; laminating the reaction flakes according to the Cu content gradient; subjecting the reaction flakes to cold pressing in a mould to obtain a prefabricated reaction block; fixing the prefabricated reaction block on the top part of the die cavity of a sand-type mould or a metal-type mould; melting pure copper in a pot resistance furnace and heating the pure copper to 1133 DEG C-1183 DEG C; and then casting to obtain the copper matrix composite material with Al2O3 content distributed in gradient on the surface of a part. The method utilizes high temperature Cu to ignite the prefabricated block containing Al powder, CuO powder and Cu powder in gradient lamination, realizes the integration of strengthening the part surface gradient and shaping the part, thereby reducing cost, strengthening the strength and the performance of high temperature creep resistance and abrasion resistance of the copper matrix composite material, and improving the quality of the part.