Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

37results about How to "Combination does not affect" patented technology

Test paper strip for detecting Porcine Parvovirus and porcine encephalitis B virus antibody

The invention discloses a test strip that is used for detecting antibodies of porcine parvovirus and porcine encephalitis B virus in one step, which comprises a supporting layer, an adsorptive layer and a protecting layer, and the adsorptive layer consists of a sample fiber layer, an adsorptive gold mark fiber layer, a fibrin membranous layer and a water absorptive material layer sequentially from a testing end; the adsorptive gold mark fiber layer adsorbs any one or two from antigen liquids of gold colloid marked SPA or gold colloid marked pure PPV and JEV; a detecting print and a contrast print are arranged on the fibrin membranous layer, the detecting print is printed with any one or two from the purified PPV and JEV antigen liquids, and the contrast print is printed with an IgG solution of ovine or rabbit anti-SPA or an IgG solution of any one or two viruses from ovine or rabbit anti-PPV and anti-JEV. The test strip of the invention has strong detecting specificity and high sensitivity, avoids additional equipment and reagents, can be operated by every person, and is particularly applicable to the quick specific antibody detection of porcine diseases on the spot.
Owner:HENAN ACAD OF AGRI SCI

Test paper strip for detecting one or more porcine virus diarrhea disease antibody

The present invention relates to a test strip which is used for testing a kind of or a plurality of kinds of disease antibodies of porcine virus diarrhea; the test strip comprises a supporting layer and a reaction reagent carrier absorbing layer which is pasted on the supporting layer; the reaction reagent carrier absorbing layer comprises a testing end fiber layer, a fiber layer of absorbing gold-labeled SPA protein or gold-labeled antigen which corresponds to the antigen to be tested, and a cellulose layer, which are arranged at the sample end in sequence, and an absorbent material layer which is positioned at the handle end; the cellulose layer contains one, two or three testing print(s) which are printed by anyone, any two or three of the purified transmissible gastroenteritis virus TGEV solution, the porcine epidemic diarrhea virus PDEV solution and the porcine rotavirus RV, and the cellulose layer also contains the contrast prints which are printed by anyone, any two or three of the anti-SPA protein IgG solution of the sheep or the rabbit or the anti-TGEV, anti-PDEV, anti-RV IgG solution of the sheep or the rabbit; and the invention provides a test strip for testing a kind of or a plurality of kinds of disease antibodies of the porcine virus diarrhea, which has the advantages of accurate and rapid detection, convenient operation and low costs.
Owner:HENAN ACAD OF AGRI SCI

Module type hydraulic classification device with variable cone angle

InactiveCN103433117AAdd adjustment parametersReduce maintenance costsWet separationEngineeringWater pipe
The invention discloses a module type hydraulic classification device with a variable cone angle. The module type hydraulic classification device with the variable cone angle is characterized in that the module type hydraulic classification device with the variable cone angle comprises three modules; the first module is composed of an upper shell body, an overflowing collecting groove and a feeding well, the upper shell body is located on the upper portion and of a conical structure, the overflowing collecting groove is formed around the outer conical surface located below a feeding port of the upper shell body, and the feeding well is arranged in the feeding port of the upper shell body in a penetrating mode through a fixing piece in the mode that the feeding well and a hydraulic classification machine body are coaxial; the second module is composed of a lower shell body, the lower shell body is located in the middle and buckled with the upper shell body, the cone angle of the lower shell body is variable, and the lower shell body is of a reverse-cone structure; the third module is composed of a hollow column section and a conical discharging section, the hollow column section is installed in the position of a discharging port formed in the bottom of the lower shell body and provided with a top water inlet pipe opening, and the conical discharging section is arranged at the bottom of the hollow column section; the upper end of the feeding well is higher than an upper edge opening of the overflowing collection groove, and the lower end of the feeding well is located above the connection portion between the second module and the third module.
Owner:HENAN POLYTECHNIC UNIV

Double rapid test strip for detecting and identifying type O and A foot and mouth disease viruses and preparation method thereof

The invention relates to a double rapid test strip for detecting and identifying type O and A foot and mouth disease viruses and a preparation method thereof. According to the test strip, on the basisof a principle of detecting antigens through double antibody sandwich ELISA, monoclonal antibodies mAbI capable of specifically identifying a type O foot and mouth disease virus and monoclonal antibodies mAbII capable of specifically identifying a type A foot and mouth disease virus are sprayed at positions of T1 and T2 detection lines of detection membrane (nitrocellulose membrane), so detectionimprints are prepared; and goat or rabbit anti-mouse IgG or SPA is sprayed at the position of a C line in a quality control area, so quality control imprints are prepared. Gold labeled antibody fibrous layers comprise an upper layer and a lower layer and are respectively used for absorbing colloidal gold labeled monoclonal antibodies mAbIII capable of specifically identifying the type O foot andmouth disease virus and colloidal gold labeled monoclonal antibodies mAbIV capable of specifically identifying the type A foot and mouth disease virus. The test strip can be used for rapidly detectingtype O and A foot and mouth disease virus (FMDV) infection. Through application of the test strip and the preparation method, operation is simple and rapid, a result is clean and is easy to identify,and the test strip and the preparation method are applicable to basic level popularization.
Owner:ZHENGZHOU UNIV +1

EB (epstein-barr) virus VCA/NA1-IgA antibody joint detection reagent and preparation method thereof

The invention provides an EB (epstein-barr) virus VCA / NA1-IgA antibody joint detection reagent. The EB virus VCA / NA1-IgA antibody joint detection reagent at least comprises a reaction membrane, an antigen pad and a gold label pad, wherein a detection line and a quality control line are labeled on the reaction membrane; the detection line contains a rat anti-human IgA monoclonal antibody; the quality control line contains biotin; the antigen pad contains a recombinant EB virus VCA antigen labeled with the biotin and a recombinant EB virus NA1 antigen labeled with the biotin; and the gold label pad contains an avidin compound containing a colloid gold label. The joint detection reagent provided by the invention is used for carrying out joint detection on VCA and NA1 so that the cost can be saved; the application and the popularization of the detection reagent are facilitated and the detection cost is reduced; and the EB virus VCA / NA1-IgA antibody joint detection reagent has the characteristics of rapidness, simplicity and convenience, accuracy and high sensitivity.
Owner:中山生物工程有限公司

EB (epstein-barr) virus NA1-IgA antibody detection reagent and preparation method thereof

The invention provides an EB (epstein-barr) virus NA1-IgA antibody detection reagent. The EB virus NA1-IgA antibody detection reagent at least comprises a reaction membrane, an antigen pad and a gold label pad, wherein a detection line and a quality control line are labeled on the reaction membrane; the detection line contains a rat anti-human IgA monoclonal antibody; the quality control line contains biotin; the antigen pad contains a recombinant EB virus NA1 antigen labeled with the biotin; and the gold label pad contains an avidin compound containing a colloid gold label. The detection reagent provided by the invention has the characteristics of rapidness, simplicity and convenience, accuracy and high sensitivity.
Owner:中山生物工程有限公司

Test strip for one-step identification of foot-and-mouth disease virus infection and vaccine-immunized animals and preparation method thereof

The invention relates to a test strip for rapidly identifying a foot-and-mouth-disease virus infected and vaccine immunized animal and a preparation method of the test strip. The test strip comprises a supporting layer and an adsorbing layer; the adsorbing layer is attached to the supporting layer; the adsorbing layer comprises a sample adsorbing fiber layer, a gold-labeled antibody fiber layer and a cellulose membrane layer sequentially arranged on the testing end and further comprises a water absorbing material layer arranged on the handle end; a detection blot and a control blot are arranged on the cellulose membrane layer; the colloidal gold labeled staphylococcus protein A is adsorbed on the gold-labeled antibody fiber layer; the detection blot is printed by one or two of a pig foot-and-mouth-disease virus protein structural VPI antigen and a non-structural protein 3ABC antigen which are expressed and purified by an escherichia coli expression system; and the control blot is printed by using a goat anti- or rabbit anti-SPA IgG antibody solution. The test strip is high in specificity, high in sensitivity, simple, direct, exact, wide in application scope, low in cost, capable of being used by professionals or non-professionals for detection at any time and any place and beneficial to popularization and application.
Owner:HENAN ACAD OF AGRI SCI

Integrated self-amplification indirect competitive immunochromatography test paper and detection method

PendingCN113063935AHigh sensitivityAvoid folding damageBiological testingCelluloseAntigen
The invention relates to integrated self-amplification indirect competitive immunochromatography test paper. The test paper comprises a supporting layer, an adsorption layer and a protective layer, wherein the adsorption layer and the protective layer are fixed on the supporting layer; the adsorption layer sequentially comprises a sample pad, a conjugate pad I, a conjugate pad II, a cellulose membrane layer and a water absorption material layer which is located at a handle end, wherein the sample pad, the conjugate pad I, the conjugate pad II and the cellulose membrane layer are sequentially distributed from a test end; a detection line and a quality control line are sequentially arranged on the cellulose membrane layer; and the conjugate pad I adsorbs a target monoclonal antibody, and the conjugate pad II adsorbs an artificial antigen marked by a nanometer material and biotin marked by the nanometer material. According to the test paper, the antibodies do not need to be marked, protective liquid is added and dried on the conjugate pad I, folding and damage of the antibodies in the antibody marking process are avoided, and the antibody dosage can be accurately controlled; and the nano-particle labeled artificial antigen is used as a probe, and each antibody intercepted on the detection line can be accurately combined with two labeled antigens, so that signal self-amplification is realized, and the sensitivity of the immunochromatography test paper is higher.
Owner:HENAN ACAD OF AGRI SCI

Self-amplification precisely-controlled indirect competitive immunochromatography test strip and application thereof

The invention relates to a 'self-amplification' and 'precise control' indirect competitive immunochromatography detection test strip which consists of two parts, namely test paper and a sample cup. The test paper comprises a supporting layer, an adsorption layer fixed on the supporting layer and a protective layer. The adsorption layer sequentially comprises a sample pad, a combination pad, a cellulose membrane layer and a water absorption material layer at a handle end from a test end. A detection line and a quality control line are sequentially arranged on the cellulose membrane layer. The conjugate pad is made of glass fiber cotton adsorbed with a nano material labeled antigen. The sample cup is a container for diluting a sample, and a target monoclonal antibody is pre-fixed in the sample cup. According to the test strip, the antibody is independently dried in the sample cup, so that folding and damage in the antibody labeling process are avoided, and the antibody dosage can be accurately controlled; and nano-particle labeled artificial antigens are used as a probe, and each antibody intercepted on a detection line can be accurately combined with two labeled antigens, so that signal self-amplification is realized, and the sensitivity of the test paper is improved.
Owner:HENAN ACAD OF AGRI SCI

A vertically-opening ice breaking high-voltage isolation switch

The invention discloses a vertically-opening ice breaking high-voltage isolation switch. The upper portion of an operation pull bar (11) is in hinged connection with one end of an ice breaking connecting rod (10). The other end of the ice breaking connecting rod (10) is in hinged connection with an ice breaking claw (7). The ice breaking claw (7) which can slide longitudinally along the surface of a conductive arm moving contact is arranged on the inner side of a moving contact area of a conductive arm (12). The tail portion of the ice breaking claw (7) is in hinged connection with the ice breaking connecting rod (10). The other end of the ice breaking connecting rod (10) is in hinged connection with the operation pull bar (11). According to the invention, through utilization of characteristics of relatively low shearing intensity of ice, motions of the moving contact pull bar simultaneously drives the motions of an ice breaking mechanism so as to realize ice breaking of the moving contact in a closing process; then the moving contact which is without ice carries out ice breaking on the static contact under working of the operation mechanism. The structure is simple and reasonable; the working is reliable; and requirements of rapid ice breaking can be satisfied.
Owner:SOUTHWEST JIAOTONG UNIV

A dual rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease virus and preparation method thereof

The invention relates to a double-link rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease viruses and a preparation method thereof. The detection card uses the principle of double-antibody sandwich ELISA to detect antigens. The monoclonal antibody mAbI that can specifically recognize the O-type foot-and-mouth disease virus and the monoclonal antibody mAbII that can specifically recognize the A-type foot-and-mouth disease virus are sprayed onto the detection membrane (nitrocellulose membrane) ) at the T1 and T2 detection line positions to prepare a detection imprint; spray goat or rabbit anti-mouse IgG or SPA to the C line position of the quality control area of ​​the detection membrane to prepare a quality control imprint. The gold-labeled antibody fiber layer is divided into upper and lower layers, which are respectively adsorbed with colloidal gold-labeled monoclonal antibody mAbIII that can specifically recognize type O foot-and-mouth disease virus and monoclonal antibody mAbIV that can specifically recognize type A foot-and-mouth disease virus. The test strip can be used for rapid detection of O-type and A-type foot-and-mouth disease virus (FMDV) infection. By applying the invention, the operation is simple and fast, the result is clear and easy to argue, and is suitable for popularization at the grassroots level.
Owner:ZHENGZHOU UNIV +1
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products