The invention relates to integrated self-amplification indirect competitive immunochromatography test paper. The test paper comprises a supporting layer, an adsorption layer and a protective layer, wherein the adsorption layer and the protective layer are fixed on the supporting layer; the adsorption layer sequentially comprises a sample pad, a conjugate pad I, a conjugate pad II, a
cellulose membrane layer and a water absorption material layer which is located at a
handle end, wherein the sample pad, the conjugate pad I, the conjugate pad II and the
cellulose membrane layer are sequentially distributed from a test end; a detection line and a
quality control line are sequentially arranged on the
cellulose membrane layer; and the conjugate pad I adsorbs a target
monoclonal antibody, and the conjugate pad II adsorbs an
artificial antigen marked by a nanometer material and
biotin marked by the nanometer material. According to the test paper, the antibodies do not need to be marked, protective liquid is added and dried on the conjugate pad I, folding and damage of the antibodies in the
antibody marking process are avoided, and the
antibody dosage can be accurately controlled; and the nano-particle labeled
artificial antigen is used as a probe, and each antibody intercepted on the detection line can be accurately combined with two labeled antigens, so that
signal self-amplification is realized, and the sensitivity of the immunochromatography test paper is higher.