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Double rapid test strip for detecting and identifying type O and A foot and mouth disease viruses and preparation method thereof

A foot-and-mouth disease virus and detection card technology, which is applied to measurement devices, analytical materials, instruments, etc., can solve problems such as antigen rapid detection cards that have not yet been seen

Active Publication Date: 2019-01-11
ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, detection of 3ABC protein antibody can be used as an important indicator for diagnosing wild virus infection. Most of the FMDV colloidal gold rapid detection cards that appear at present are based on test strips for detecting 3ABC protein antibody, such as patents CN105954514A, CN202814986U, CN101236204, CN1798976B, etc. It belongs to the colloidal gold rapid test card for detecting FMDV antibodies; while most of the FMDV antigen rapid test cards that have appeared so far are for the detection of O-type FMDV, there is no antigen rapid test card that can detect O-type and A-type FMDV at the same time

Method used

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  • Double rapid test strip for detecting and identifying type O and A foot and mouth disease viruses and preparation method thereof
  • Double rapid test strip for detecting and identifying type O and A foot and mouth disease viruses and preparation method thereof
  • Double rapid test strip for detecting and identifying type O and A foot and mouth disease viruses and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0065] Such as Figure 1-2 As shown, a double-linked rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease viruses, including a jam and a test strip, the test strip includes a support plate 1 and an adsorption layer fixed on the support plate, and the adsorption layer is from the test Starting from the end, there are sample pad 3, gold label pad, detection film 2 and water-absorbing pad 9 in sequence. Colloidal gold-labeled anti-type O foot-and-mouth disease virus monoclonal antibody mAbIII is adsorbed on the pad, colloidal gold-labeled anti-type A foot-and-mouth disease virus monoclonal antibody mAbIV is adsorbed on the lower layer of gold-labeled pad, and the detection membrane is equipped with goat antibody or rabbit anti-mouse The quality control line C 6 printed by IgG antibody or Staphylococcus aureus SPA, the test line T1 7 printed by anti-O-FMDV monoclonal antibody mAbI, and the test line T2 8 printed by anti-A-FMDV monoclonal a...

Embodiment 2

[0070] The preparation of the dual rapid detection card of O-FMDV and A-FMDV of the present invention: first prepare O-FMDV immune antigen and A-FMDV immune antigen, and then prepare anti-O-FMDV monoclonal antibody and A-FMDV monoclonal antibody, and Screening of monoclonal antibodies recognizing O-FMDV and A-FMDV. Among them, O-FMDV monoclonal antibody mAbIII and A-FMDV monoclonal antibody mAbIV are used to prepare gold-labeled antibodies, and monoclonal antibody mAbI that specifically recognizes O-FMDV and is different from mAbIII's epitope is used for printing O-FMDV detection Line blot T1 "│", the monoclonal antibody mAbII that specifically recognizes A-FMDV and is different from the epitope recognized by mAbIV is used to print A-FMDV detection line blot T2 "│"; secondly, prepare goat or rabbit anti-mouse IgG antibody or Staphylococcus aureus SPA, used to print the quality control line blot C "│"; then assemble the prepared sample pad, gold standard pad, detection membrane...

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Abstract

The invention relates to a double rapid test strip for detecting and identifying type O and A foot and mouth disease viruses and a preparation method thereof. According to the test strip, on the basisof a principle of detecting antigens through double antibody sandwich ELISA, monoclonal antibodies mAbI capable of specifically identifying a type O foot and mouth disease virus and monoclonal antibodies mAbII capable of specifically identifying a type A foot and mouth disease virus are sprayed at positions of T1 and T2 detection lines of detection membrane (nitrocellulose membrane), so detectionimprints are prepared; and goat or rabbit anti-mouse IgG or SPA is sprayed at the position of a C line in a quality control area, so quality control imprints are prepared. Gold labeled antibody fibrous layers comprise an upper layer and a lower layer and are respectively used for absorbing colloidal gold labeled monoclonal antibodies mAbIII capable of specifically identifying the type O foot andmouth disease virus and colloidal gold labeled monoclonal antibodies mAbIV capable of specifically identifying the type A foot and mouth disease virus. The test strip can be used for rapidly detectingtype O and A foot and mouth disease virus (FMDV) infection. Through application of the test strip and the preparation method, operation is simple and rapid, a result is clean and is easy to identify,and the test strip and the preparation method are applicable to basic level popularization.

Description

technical field [0001] The invention relates to a detection device for identifying two kinds of livestock diseases at the same time, in particular to a double-link rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease viruses and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (foot-and-mouth disease, FMD) is an acute, febrile, highly contagious infectious disease of cloven-hoofed animals (pigs, cattle, sheep) caused by foot-and-mouth disease virus (FMDV), susceptible There are more than 70 species of animals. The disease has the characteristics of many types of infected animals, fast transmission speed, strong infectivity, and huge economic losses caused. Foot-and-mouth disease is one of the 25 kinds of zoonotic infectious and parasitic diseases that must be declared in the "OIE Diseases, Infection and Infestation List". Immunization against animal diseases. [0003] FMDV belongs to the small RNA ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543G01N33/531
CPCG01N33/531G01N33/54306G01N33/56983
Inventor 张改平刘运超王爱萍刘东民赵建国陈玉梅石海宁周景明王方雨王彦伟
Owner ZHENGZHOU UNIV
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