5 results about "Fmd virus" patented technology

The invention relates to a recombinant CHO cell strain capable of efficiently inducing and expressing swine foot-and-mouth disease VLP as well as a preparation method and application of the recombinant CHO cell strain. The method comprises the following steps: firstly, screening a monoclonal cell strain capable of stably inducing expression of FMDV 3C protease by taking CHO-B40 (GS defective strain) as a host cell and taking hygromycin and GS as screening markers in a CHO cell strain stable transformation mode, naming the monoclonal cell strain as CHO-FMDV-3C-3D6 cell strain, and then, carrying out recombinant expression of FMDV 3C protease by taking the CHO-FMDV-3C-3D6 cell strain as a host cell. The CHO-FMDV-3C-VLP-4D8 cell strain capable of stably expressing the P12A protein of the foot and mouth disease virus is screened through MSX, the cell strain can only induce expression of 3C protease under the action of an inducer GLP-1 in the culture process, the expressed 3C protease cuts the expressed P12A protein of the FMDV, the cut protein is self-assembled to form FMDV VLP, and the FMDV VLP is separated from the FMDV VLP. The purified VLP and an ISA201 adjuvant are emulsified to prepare an FMDV vaccine, and the FMDV vaccine has good immunogenicity and is beneficial to the development of the swine foot-and-mouth disease virus vaccine industry.

The invention belongs to the field of biology, and particularly relates to a novel application of a compound Sotagliflozin for resisting foot and mouth disease viruses. The invention accidentally discovers that the compound Sotagliflozin can significantly inhibit the expression of foot and mouth disease virus RNA polymerase 3D protein, reduce the half histiocyte infection dose (TCID50) of the foot and mouth disease virus, can significantly reduce the replication ability of the foot and mouth disease virus, has the effect of antagonizing foot and mouth disease virus infection, can be used for preparing the anti-foot and mouth disease virus medicine, and can be used for preparing the anti-foot and mouth disease virus medicine. Good application prospects are realized.

This invention discloses a recombinant BCG vaccine expressing a multi-epitope fusion peptide of foot-and-mouth disease virus (FMD) types O and A and its application, belonging to the fields of biotechnology and veterinary vaccines. The amino acid sequence of the multi-epitope fusion peptide is shown in SEQ ID NO:1, containing dominant immune T-cell and B-cell antigenic epitopes from multiple circulating FMD virus types O and A, and fused with a mycobacterial signal peptide at the N-terminus. This invention successfully constructed the recombinant strain rBCG-MIPGA by codon optimization of the fusion gene, cloning it into the pMV306 vector, and electroporating it into BCG. This recombinant BCG can simultaneously stimulate high-titer specific antibodies and significant T-cell immune responses against FMD virus types O and A, exhibiting durable and broad-spectrum protective potential, and has significant application value in the preparation of safe, efficient, and broad-spectrum FMD vaccines.

Compositions for prevention of Foot and Mouth Disease (FMD) are provided, comprising an antigen component in the amount equivalent to 0.5-20 μg FMD virus and an adjuvant component comprising oil, an immunostimulatory oligonucleotide, and a polycationic carrier. Methods of using the composition, as well as the methods of reducing FMD persistence are also provided.

This disclosure provides a method for preventing foot lesions caused by FMD virus infection in ruminants through the use of a vaccine presented as an emulsion containing one or more adjuvants and viral antigens.