31 results about "Fmd virus" patented technology

The present invention relates to modified poxviral vectors and to methods of making and using the same. In particular, the invention relates to recombinant avipox that expresses gene products of foot and mouth disease virus (FMDV), and to compositions or vaccines that elicit immune responses directed to FMDV gene products and which can confer protective immunity against infection by FMDV.

The invention provides a chimeric protein, a virus-like particle and application thereof and belongs to the field of molecular biology. The chimeric protein is prepared by the following steps: replacing one, two or three of four sites in a porcine circovirus 2 type Cap protein by a main B cell epitope of a porcine O-shaped foot-and-mouth disease virus VP1 protein to obtain 58th-66th amino acid residues, 72nd-94th amino acid residues, 122nd-147th amino acid residues and 162nd-197th amino acid residues. The chimeric protein can form the chimeric virus-like particle after soluble expression, shows the main B cell epitope of the porcine O-shaped foot-and-mouth disease virus VP1 protein on the surface of the chimeric virus-like particle, has very good immunogenicity to both PCV2 and FMDV and can generate a high antibody to the PCV2 and the FMDV, by once immune.

The invention provides a foot-and-mouth disease O-type PanAsia-2 pedigree reserve vaccine strain as well as a construction method and application thereof, and belongs to the technical field of veterinary drug biological products. The foot-and-mouth disease recombinant virus rHN / TUR09 / VP1 is obtained by taking an O / HN / CHA / 93 virus strain as a skeleton and embedding a VP1 gene of O / TURR / 5 / 2009. The rHN / TUR09 / VP1 not only is highly matched with antigens of a foot-and-mouth disease O-type PanAsia pedigree strain and an Ind-2001 pedigree strain which are relatively close to the genetic relationship, but also is matched with antigens of a foot-and-mouth disease O-type Cathay pedigree strain and a Mya-98 pedigree strain which are relatively far from the genetic relationship, so that the rHN / TUR09 / VP1 can be used for well immunizing, preventing and controlling the prevalence of the current O-type multi-pedigree FMD virus strain in China; and the strain can be used as a strategic reserve vaccine strain for effective prevention and control of O / Panaa-2 pedigree foot-and-mouth disease in border areas of China.

The present invention relates to modified poxviral vectors and to methods of making and using the same. In particular, the invention relates to recombinant modified vaccinia virus Ankara-based (MVA-based) vaccine against FMDV infection and to related products, methods and uses. Specifically, the present invention relates to genetically engineered (recombinant) MVA vectors comprising at least one heterologous nucleotide sequence encoding an antigenic determinant of a FMDV protein. The invention also relates to products, methods and uses thereof, e.g., suitable to induce a protective immune response in a subject.

The invention provides monoclonal antibodies 6G6 and 7D7 which can specifically bind with different genetic topology O-type foot-and-mouth disease (FMD) viruses, and an ELISA detection kit prepared from the monoclonal antibodies 6G6 and 7D7 can be used for carrying out high-sensitivity detection on the different genetic topology O-type FMD viruses.

A multiplex-PCR (polymerase chain reaction) kit for detecting six viruses of sheep and goats simultaneously comprises six pairs of specific primers, wherein the primers used for detection of the bluetongue virus are BTV-F and BTV-R respectively; the primers for detection of the foot and mouth disease virus are FMDV-F and FMDV-R respectively; the primers for detection of the peste des petits ruminants virus are PPRV-F and PPRV-R respectively; the primers for detection of the sheep pox virus are SPPV-F and SPPV-R respectively; the primers for detection of the goat pox virus are GTPV-F and GTPV-R respectively; the primers for detection of the sore mouth disease virus are ORFV-F and ORFV-R respectively. The sequences of the primers are nucleotide sequences shown in SEQ ID NO: 1-12. The multiplex-PCR kit can be used for detecting nucleic acid of the six viruses including the bluetongue virus, the foot and mouth disease virus, the peste des petits ruminants virus, the sheep pox virus, the goat pox virus and the sore mouth disease virus in sheep and goat clinical samples and has the characteristics of high sensitivity, high specificity, simple operation and reduction of detection time.

The present invention relates to the field of molecular immunology. Although conventional vaccines such as attenuated foot-and-mouth disease vaccines and inactivated vaccines currently used have good immunogenicity, there are problems such as strong virus virulence, incomplete virus inactivation, and live virus escape from the preparation factory. Unsafe factors have prompted people to seek a safer and more effective FMD vaccine. The present invention utilizes phage surface display technology to screen effective FMDV epitopes, and provides a molecular mimic peptide of O-type foot-and-mouth disease virus antigen epitope, and the present invention also provides that the mimic peptide can be used in the preparation of immunogen and porcine foot-and-mouth disease epitope peptide vaccine Applications. The FMDV epitope peptide vaccine of the present invention has the antigenicity of the target molecule without toxicity, is very safe, and has low cost in large-scale production, and has good economic and social benefits.

The present invention relates to modified poxviral vectors and to methods of making and using the same. In particular, the invention relates to recombinant modified vaccinia virus Ankara-based (MVA-based) vaccine against FMDV infection and to related products, methods and uses. Specifically, the present invention relates to genetically engineered (recombinant) MVA vectors comprising at least one heterologous nucleotide sequence encoding an antigenic determinant of a FMDV protein. The invention also relates to products, methods and uses thereof, e.g., suitable to induce a protective immune response in a subject.

The invention discloses a foot-and-mouth disease virus-like particle, a preparation method, a vaccine composition and application. The vaccine composition of the present invention is composed of O-type foot-and-mouth disease virus-like particles composed of foot-and-mouth disease virus structural proteins VP4, VP2, VP3, and VP1 and / or Asian type 1 foot-and-mouth disease virus-like particles and / or foot-and-mouth disease virus-like particles composed of foot-and-mouth disease structural proteins VPO, VP3, and VP1 The type A foot-and-mouth disease virus-like particles composed of structural proteins VP0, VP3, and VP1 are composed of an adjuvant, which has a stable structure and reasonable components. It can quickly form specific antibodies, significantly increase the duration of immunity, and maintain long-term immune protection.

The present application relates to a method for preparing a foot-and-mouth disease (FMD) vaccine, comprising the following steps: (i) obtaining cell culture media containing FMD virus; (ii) separating and purifying the cell culture media containing FMD virus by an integrated filtration system with two membranes in combination; and (iii) collecting the concentrated solution containing FMD virus obtained in step (ii). The present application also relates to an FMD vaccine prepared by the method described herein and use thereof in the manufacture of a medicament for preventing animal FMD. The present application further relates to an apparatus for preparing an FMD vaccine, which comprises an integrated filtration system with two membranes in combination.

The invention provides a Seneca recombinant virus of recombinant O-type foot-and-mouth disease virus epitope gene, a recombinant vaccine, a preparation method and application thereof, and relates to the technical field of genetic engineering. The present invention obtains the full-length cDNA of SVV / FJ / 001 strain, and performs deletion and mutation transformation on its 5'UTR, and at the same time fuses the recombinant epitope gene of O-type FMDV into the SVA cDNA to construct the recombinant foot-and-mouth disease antigen epitope The Seneca recombinant virus, which can express the fused foot-and-mouth disease B-cell epitope and T-cell epitope, and the expression product has good reactogenicity, and the pathogenicity of the recombinant virus is significantly reduced or even non-pathogenic to pigs It can significantly improve the biological safety of the strain; the prepared inactivated vaccine has good immunogenicity, and can effectively stimulate SVA neutralizing antibodies while also producing specific antibodies against FMDV, which can be used for Seneca virus and Control of one or more non-Seneca viruses.

The invention relates to the application of buquinal in the preparation of medicines for preventing foot-and-mouth disease virus infection, and belongs to the technical field of veterinary medicines. The application of the invention can provide a class of highly efficient, safe and quality-controllable anti-foot-and-mouth disease virus medicine for further controlling the spread of foot-and-mouth disease.

The invention discloses a foot-and-mouth disease virus non-structural protein monoclonal antibody 7D7, a foot-and-mouth disease virus non-structural protein antibody detection kit containing the monoclonal antibody and an application thereof. The antibody detection kit provided by the invention has high sensitivity and can obviously widen the linear range of detection; the detection is not limited by animal species, and is suitable for large-scale screening of foot-and-mouth disease diseases of artiodactyl animals. The vaccine prepared by the conjugate containing the monoclonal antibody 7D7 can remove non-structural proteins, and can avoid the interference of FMDV non-structural protein antibodies after immunizing animals, thereby accurately distinguishing infected animals from immunized animals.

The invention relates to a double-link rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease viruses and a preparation method thereof. The detection card uses the principle of double-antibody sandwich ELISA to detect antigens. The monoclonal antibody mAbI that can specifically recognize the O-type foot-and-mouth disease virus and the monoclonal antibody mAbII that can specifically recognize the A-type foot-and-mouth disease virus are sprayed onto the detection membrane (nitrocellulose membrane) ) at the T1 and T2 detection line positions to prepare a detection imprint; spray goat or rabbit anti-mouse IgG or SPA to the C line position of the quality control area of ​​the detection membrane to prepare a quality control imprint. The gold-labeled antibody fiber layer is divided into upper and lower layers, which are respectively adsorbed with colloidal gold-labeled monoclonal antibody mAbIII that can specifically recognize type O foot-and-mouth disease virus and monoclonal antibody mAbIV that can specifically recognize type A foot-and-mouth disease virus. The test strip can be used for rapid detection of O-type and A-type foot-and-mouth disease virus (FMDV) infection. By applying the invention, the operation is simple and fast, the result is clear and easy to argue, and is suitable for popularization at the grassroots level.

The invention discloses a monoclonal antibody blocking enzyme-linked immunosorbent assay (ELISA) kit and a method for detecting the nonstructural protein (NSP) antibody of a foot-and-mouth disease virus (FMDV) (FMD NSP B-ELISA); the kit comprises ELISA reaction plates, serum diluent, 25 times concentrated detergent, substrate solution, 100* concentrated ELISA detecting antibody, stop buffer, positive control serum and negative control serum; the ELISA reaction plates are two 96-pore high-affinity ELISA reaction plates, firstly 6* groups of amino acid monoclonal antibody or NSP 2C polyclonal antibody, and then FMDV 3ABC or 2C3AB NSP which is expressed by pronucleus and is provided with 6* groups of amino acid labels is captured through the monoclonal antibody or the polyclonal antibody; and compared with other similar kits, the method has higher coincidence rate and higher positive serum detection rate, and is applicable to detecting the serum of cattle, sheep, pigs and other susceptible animals.

The present invention relates to the technical field of molecular biology, and relates to an artificial attenuated strain O / Akesu / 58 CE39B genome sequence, primers and applications thereof. The artificially attenuated strain O / Akesu / 58 CE39B genome sequence is as follows: Shown in SEQ ID No.1. The present invention discloses the full genome sequence of the artificially attenuated strain O / Akesu / 58 CE39B of foot-and-mouth disease virus for the first time, which is helpful for the reverse genetics research of the virus, and is for further research on the proliferation mechanism, virus titer and pathogenic mechanism of FMDV in cells It lays the foundation for FMDV-marked vaccines, which is conducive to the study of virus evolution, pathogenicity, and molecular epidemiology; and the artificially attenuated strain of foot-and-mouth disease virus O / Akesu / 58 CE39B rescued virus has good safety and effectiveness, and is expected to be used as a vaccine. The next-generation genetically engineered vaccine is used for the prevention and control of foot-and-mouth disease, and has broad prospects for research and industrialization.

The invention relates to a foot-and-mouth disease virus antibody, nucleic acid encoding it and application. The foot-and-mouth disease virus antibody includes a heavy chain and a light chain, wherein the amino acid sequence of the heavy chain is shown in SEQ ID No.1, and the amino acid sequence of the light chain is shown in SEQ ID No.2. This is the first time that the neutralization mechanism of the FMD virus antibody is found by enhancing the stability of the FMD virus capsid to neutralize the FMD virus infection.

The invention relates to a duck tembusu virus transient transfection replicon carrying ranilla luciferase as well as a construction method and application of the duck tembusu virus transient transfection replicon. The replicon is characterized in that a ranilla luciferase reporter gene is linked after a tembusu virus deletes an encoding structure gene and the sequences of 38 amino acids in front of N-terminal of C protein are encoded; a sequence FMDV2A for encoding self-splicing peptide of a foot and mouth disease virus is inserted behind Rluc; an eukaryotic promoter CMV and a prokaryote promoter T7 are added to the upstream of the 5' UTR sequence of the tembusu virus; hepatitis delta virus ribozyme sequence HDVr and SV40poly(A) sequence are added to the downstream of 3' UTR; the obtainedreplicon can accurately and quantitatively reflect replication conditions of the replicon in cells and can be conveniently applied to research of replication mechanisms of the tembusu virus or even other flaviviruses, antiviral drug screening, virus and protein interaction and the like.