31 results about "Fmd virus" patented technology

The present invention relates to modified poxviral vectors and to methods of making and using the same. In particular, the invention relates to recombinant modified vaccinia virus Ankara-based (MVA-based) vaccine against FMDV infection and to related products, methods and uses. Specifically, the present invention relates to genetically engineered (recombinant) MVA vectors comprising at least one heterologous nucleotide sequence encoding an antigenic determinant of a FMDV protein. The invention also relates to products, methods and uses thereof, e.g., suitable to induce a protective immune response in a subject.

The present invention relates to the field of molecular immunology. Although conventional vaccines such as attenuated foot-and-mouth disease vaccines and inactivated vaccines currently used have good immunogenicity, there are problems such as strong virus virulence, incomplete virus inactivation, and live virus escape from the preparation factory. Unsafe factors have prompted people to seek a safer and more effective FMD vaccine. The present invention utilizes phage surface display technology to screen effective FMDV epitopes, and provides a molecular mimic peptide of O-type foot-and-mouth disease virus antigen epitope, and the present invention also provides that the mimic peptide can be used in the preparation of immunogen and porcine foot-and-mouth disease epitope peptide vaccine Applications. The FMDV epitope peptide vaccine of the present invention has the antigenicity of the target molecule without toxicity, is very safe, and has low cost in large-scale production, and has good economic and social benefits.

The present invention relates to modified poxviral vectors and to methods of making and using the same. In particular, the invention relates to recombinant modified vaccinia virus Ankara-based (MVA-based) vaccine against FMDV infection and to related products, methods and uses. Specifically, the present invention relates to genetically engineered (recombinant) MVA vectors comprising at least one heterologous nucleotide sequence encoding an antigenic determinant of a FMDV protein. The invention also relates to products, methods and uses thereof, e.g., suitable to induce a protective immune response in a subject.

The invention discloses a foot-and-mouth disease virus-like particle, a preparation method, a vaccine composition and application. The vaccine composition of the present invention is composed of O-type foot-and-mouth disease virus-like particles composed of foot-and-mouth disease virus structural proteins VP4, VP2, VP3, and VP1 and / or Asian type 1 foot-and-mouth disease virus-like particles and / or foot-and-mouth disease virus-like particles composed of foot-and-mouth disease structural proteins VPO, VP3, and VP1 The type A foot-and-mouth disease virus-like particles composed of structural proteins VP0, VP3, and VP1 are composed of an adjuvant, which has a stable structure and reasonable components. It can quickly form specific antibodies, significantly increase the duration of immunity, and maintain long-term immune protection.

The invention provides a Seneca recombinant virus of recombinant O-type foot-and-mouth disease virus epitope gene, a recombinant vaccine, a preparation method and application thereof, and relates to the technical field of genetic engineering. The present invention obtains the full-length cDNA of SVV / FJ / 001 strain, and performs deletion and mutation transformation on its 5'UTR, and at the same time fuses the recombinant epitope gene of O-type FMDV into the SVA cDNA to construct the recombinant foot-and-mouth disease antigen epitope The Seneca recombinant virus, which can express the fused foot-and-mouth disease B-cell epitope and T-cell epitope, and the expression product has good reactogenicity, and the pathogenicity of the recombinant virus is significantly reduced or even non-pathogenic to pigs It can significantly improve the biological safety of the strain; the prepared inactivated vaccine has good immunogenicity, and can effectively stimulate SVA neutralizing antibodies while also producing specific antibodies against FMDV, which can be used for Seneca virus and Control of one or more non-Seneca viruses.

The invention discloses a foot-and-mouth disease virus non-structural protein monoclonal antibody 7D7, a foot-and-mouth disease virus non-structural protein antibody detection kit containing the monoclonal antibody and an application thereof. The antibody detection kit provided by the invention has high sensitivity and can obviously widen the linear range of detection; the detection is not limited by animal species, and is suitable for large-scale screening of foot-and-mouth disease diseases of artiodactyl animals. The vaccine prepared by the conjugate containing the monoclonal antibody 7D7 can remove non-structural proteins, and can avoid the interference of FMDV non-structural protein antibodies after immunizing animals, thereby accurately distinguishing infected animals from immunized animals.

The invention relates to a double-link rapid detection card for detecting and distinguishing O-type and A-type foot-and-mouth disease viruses and a preparation method thereof. The detection card uses the principle of double-antibody sandwich ELISA to detect antigens. The monoclonal antibody mAbI that can specifically recognize the O-type foot-and-mouth disease virus and the monoclonal antibody mAbII that can specifically recognize the A-type foot-and-mouth disease virus are sprayed onto the detection membrane (nitrocellulose membrane) ) at the T1 and T2 detection line positions to prepare a detection imprint; spray goat or rabbit anti-mouse IgG or SPA to the C line position of the quality control area of ​​the detection membrane to prepare a quality control imprint. The gold-labeled antibody fiber layer is divided into upper and lower layers, which are respectively adsorbed with colloidal gold-labeled monoclonal antibody mAbIII that can specifically recognize type O foot-and-mouth disease virus and monoclonal antibody mAbIV that can specifically recognize type A foot-and-mouth disease virus. The test strip can be used for rapid detection of O-type and A-type foot-and-mouth disease virus (FMDV) infection. By applying the invention, the operation is simple and fast, the result is clear and easy to argue, and is suitable for popularization at the grassroots level.

The invention discloses a monoclonal antibody blocking enzyme-linked immunosorbent assay (ELISA) kit and a method for detecting the nonstructural protein (NSP) antibody of a foot-and-mouth disease virus (FMDV) (FMD NSP B-ELISA); the kit comprises ELISA reaction plates, serum diluent, 25 times concentrated detergent, substrate solution, 100* concentrated ELISA detecting antibody, stop buffer, positive control serum and negative control serum; the ELISA reaction plates are two 96-pore high-affinity ELISA reaction plates, firstly 6* groups of amino acid monoclonal antibody or NSP 2C polyclonal antibody, and then FMDV 3ABC or 2C3AB NSP which is expressed by pronucleus and is provided with 6* groups of amino acid labels is captured through the monoclonal antibody or the polyclonal antibody; and compared with other similar kits, the method has higher coincidence rate and higher positive serum detection rate, and is applicable to detecting the serum of cattle, sheep, pigs and other susceptible animals.

The invention relates to a foot-and-mouth disease virus antibody, nucleic acid encoding it and application. The foot-and-mouth disease virus antibody includes a heavy chain and a light chain, wherein the amino acid sequence of the heavy chain is shown in SEQ ID No.1, and the amino acid sequence of the light chain is shown in SEQ ID No.2. This is the first time that the neutralization mechanism of the FMD virus antibody is found by enhancing the stability of the FMD virus capsid to neutralize the FMD virus infection.