Bi-recombinant active carrier vaccine of co-expression pig gyrate virus and foot and mouth disease virus
A technology of porcine circovirus and foot-and-mouth disease virus, applied in the direction of viral peptides, antibody medical ingredients, medical preparations containing active ingredients, etc., can solve the problems of no effective treatment methods and no effective vaccines available
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[0182] 1. Preparation and transformation of E. coli competent cells, plasmid extraction and restriction enzyme digestion, DNA fragment recovery, linear DNA fragment connection, recombinant plasmid screening and identification, PCR amplification reaction, etc. Refer to Jin Dongyan and Li Mengfeng Translated "Molecular Cloning Experiment Guide" second edition, Science Press (1992) related chapters.
[0183] 1.1 Preparation of E. coli competent cells
[0184] Under aseptic conditions, pick the strains stored in the refrigerator at -70°C, streak them and inoculate them on LB agar plates without ampicillin (Amp), and invert them at 37°C for about 16 hours. Pick a single colony, inoculate it into 3ml of LB culture medium without antibiotics, and cultivate overnight at 37°C with shaking. The next day, 1ml of bacterial culture was aseptically drawn and added to 100ml of Amp-free LB culture that was preheated to 37°C, and cultured at 37°C with vigorous shaking (200r / min). Cultivate to OD 6...
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