Subunit vaccine for porcine reproductive and respiratory syndrome as well as preparation method and application of subunit vaccine
A subunit vaccine and porcine PRRS technology, which is applied in the field of porcine PRRS subunit vaccine and its preparation, can solve problems such as irritation, and achieve the effects of improving immunogenicity, good protection effect, and easier separation and purification
Active Publication Date: 2020-11-24
WUHAN KEQIAN BIOLOGY CO LTD
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Abstract
The invention relates to the fields of genetic engineering and veterinary biological pharmacy, and in particular discloses a subunit vaccine for porcine reproductive and respiratory syndrome as well as a preparation method and application of the subunit vaccine. The invention firstly provides a fusion protein, wherein an amino acid sequence of the fusion protein is as shown in SEQ ID No. 1; and then the invention provides the subunit vaccine for the porcine reproductive and respiratory syndrome, wherein the subunit vaccine comprises the fusion protein. The fusion protein in the subunit vaccinefor the porcine reproductive and respiratory syndrome is obtained by conducting fusion expression on an ORF5 full-length gene of a PRRSV NADC30-like strain, which is optimized by virtue of a codon, and a gene of pseudomonas aeruginosa, from which a Domain III structural Domain is removed, and by virtue of an insect baculovirus/ insect cell expression system. The vaccine provided by the inventionis good in protective effect, capable of inducing effective humoral immunity and cellular immunity and is suitable for clinical prevention of the reproductive and respiratory syndrome.
Application Domain
SsRNA viruses positive-senseVirus peptides +15
Technology Topic
Genetic engineeringCellular immunity +12
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