6 results about "Protein virus" patented technology

The invention provides a porcine circovirus type 5 Cap protein, a virus-like particle, an elisa plate, an ELISA kit and application thereof, and belongs to the technical field of animal viruses. The invention provides a porcine circovirus type 5 Cap protein with an amino acid sequence as shown in SEQ ID NO. 1. The porcine circovirus type 5 Cap protein provided by the invention has the advantages of stable structure and good antigenicity, can be specifically combined with a circovirus type 5 antibody in a serum sample, and can be self-assembled into 60 virus-like particles. The ELISA plate and the ELISA kit for detecting the porcine circovirus type 5, provided by the invention, have the advantages of strong specificity and high sensitivity, and can accurately detect whether a blood sample contains the porcine circovirus type 5 or not. The porcine circovirus type 5 virus-like particle provided by the invention has the advantages of high yield, good uniformity and strong stability, and can be subsequently prepared into a porcine circovirus type 5 subunit vaccine.

The invention belongs to the technical field of Alzheimer's disease, and particularly relates to application of a Borna disease virus type 1 virus peptide and a derivative of the Borna disease virus type 1 virus peptide to preparation of a medicine for preventing and / or treating Alzheimer's disease, the virus peptide is X protein, and the derivative of the virus peptide is X protein derived peptide PX3, the derivative PX3 of the virus peptide has a remarkable protection effect on SH-SY5Y cell injury induced by A beta, and a new strategy is provided for preventing and / or treating the Alzheimer disease.

The application discloses a kind of electrochemical biomolecular sensor and construction method based on DNA molecular pendulum, including buffer solution, electrode, DNA double-stranded pendulum rod, the electrode, DNA double-stranded pendulum rod is set in buffer solution;Step voltage reduction signal molecule is inserted on the phosphate skeleton of the DNA double-stranded pendulum rod;One end of the DNA double-stranded pendulum rod is connected on electrode, and one single strand on the other end of the DNA double-stranded pendulum rod is connected with target aptamer.The application has wider detection range, higher sensitivity and lower detection limit, and has important significance for detecting protein, virus, nucleic acid and some small molecule substances.

The application provides a purification method of foot-and-mouth disease virus capsid protein virus-like particles, and the purification method comprises the following steps: a fusion protein containing P1-2A is subjected to enzymolysis by 3C and TEV proteases to obtain an enzymolysis liquid; the enzymolysis liquid is subjected to nickel column purification by a nickel column to obtain foot-and-mouth disease virus capsid protein virus-like particle 5S protomers; and then foot-and-mouth disease virus capsid protein virus-like particles VLPs can be obtained by self-assembly. The purity of the foot-and-mouth disease virus capsid protein virus-like particles obtained by the purification method of the application is more than 90%, and the yield is more than 95%.

The present application relates to a model for recruiting and concentrating NuRD complex in the nucleus by transcription factor SALL4 and application. The method for constructing the model for recruiting and concentrating NuRD complex in the nucleus by transcription factor SALL4 comprises the following steps: 1) constructing a SALL4-fluorescent protein fusion protein coding fragment; 2) constructing a SALL4-fluorescent protein fusion protein virus expression vector; 3) packaging a virus for expressing SALL4-fluorescent protein fusion protein; 4) primary separation / in vitro culture of target cells; the target cells in 4) are infected by the virus vector constructed in 3), that is, SALL4-fluorescent protein fusion protein is expressed in the target cells, and a cell model for recruiting and concentrating NuRD complex in the nucleus by transcription factor SALL4 is obtained. According to the scheme provided in the present application, a model for recruiting and concentrating NuRD complex in the nucleus can be established. The model provided in the present application can be applied to in situ electron microscope structure analysis of NuRD in cells.

The invention discloses a therapeutic vaccine for human papilloma virus infection and a preparation method thereof. In particular to a composition for preventing and / or treating diseases caused by HPV infection. The composition comprises DC cells loaded with HPV antigens and exosomes. The invention also discloses a preparation method of the composition. The therapeutic vaccine provided by the invention comprises a SiHa cell exosome, an MS751 cell exosome and a human DC cell loaded with HPV VLPs (L1 protein virus-like particles of HPV). Experiments show that the therapeutic vaccine can induce and significantly enhance HPV specific CTL immune response, has an obvious killing effect on cervical cancer cells, can enhance anti-tumor immune response, and enhances the immunity of an organism to diseases, thereby achieving a therapeutic effect. The therapeutic vaccine can effectively treat HPV infection and diseases caused by the HPV infection, or prevent deterioration of the HPV infection and the diseases caused by the HPV infection.