46 results about "Protein virus" patented technology

The present invention relates to a synthetic plant-optimized nucleic acid molecule having a Norwalk virus capsid protein coding nucleotide sequence, and nucleic acid constructs, host cells, expression systems, and plants having the plant-optimized Norwalk virus nucleic acid molecule. The present invention also relates to a method of producing Norwalk virus capsid protein virus-like particles in a transgenic plant or transgenic plant seed transformed with a plant-optimized nucleic acid molecule encoding Norwalk virus capsid protein. The plant or a component thereof can be administered to a subject under conditions effective to immunize the subject against disease resulting from infection by a Norovirus, including Norwalk virus. An oral vaccine for immunization of a subject against Norwalk virus infection is also disclosed.

The invention relates to escherichia coli-derived single-plasmid-tandem soluble coexpression foot-and-mouth disease virus capsid proteins VP0 (which is a VP4 and VP2 fusion gene), VP1 and VP3, and a foot-and-mouth disease virus capsid protein virus-like particle preparation method. Foot-and-mouth disease virus capsid protein virus-like particles can be used for preparation of a foot-and-mouth disease vaccine. According to the method, a plurality of aspects of escherichia coli-derived soluble coexpression foot-and-mouth disease virus capsid protein are studied, by comprehensive use of tandem coexpression and SUMO(suggested upper merged ontology) technology with a tag for soluble coexpression of the foot-and-mouth disease virus capsid proteins VP0 (which is the VP4 and VP2 fusion gene), VP1 and VP3, the ultimate objective protein accounts for about 20% of total bacterial protein, and the foot-and-mouth disease virus capsid proteins obtained by purification can be successfully assembled into the virus like particles.

The invention supplies a manufacturing technique of IBV-HI antigen, the preparation process of antigen is that: the virus fluid of containing the protein virus is isolated from chicken embryo allantois fluid of containing respiratory type IBV the virus and blending with clostridium filtrate of rabbits A type wei surname as a culture medium, after two hours water bath action with 37deg.C, put the fluid into the 4deg.C fridge for 48 hours, through freeze drying to get antigen, the related centrifuge divided into two centrifugal, first the mixed fluid through high speed centrifugation, choose the supernatant after the centrifugation; then it progress the two centrifugation as ultracentrifugation, the time is 2-3h, the rotation rate of centrifugation is 25000-35000g, abandon the supernatant after the centrifugation, and use deposit suspension to obtain virus fluid. Using this invention method, infectious bronchitis HI antigen of respiratory system has relatively low cost, type specificity, high sensitivity, stability feature, it makes HI method to detect respiratory infectious bronchitis feasible, and enhance the credibility and maneuverability of its detection.