2 results about "Protein virus" patented technology

The application provides a purification method of foot-and-mouth disease virus capsid protein virus-like particles, and the purification method comprises the following steps: a fusion protein containing P1-2A is subjected to enzymolysis by 3C and TEV proteases to obtain an enzymolysis liquid; the enzymolysis liquid is subjected to nickel column purification by a nickel column to obtain foot-and-mouth disease virus capsid protein virus-like particle 5S protomers; and then foot-and-mouth disease virus capsid protein virus-like particles VLPs can be obtained by self-assembly. The purity of the foot-and-mouth disease virus capsid protein virus-like particles obtained by the purification method of the application is more than 90%, and the yield is more than 95%.

The application discloses a kind of electrochemical biomolecular sensor and construction method based on DNA molecular pendulum, including buffer solution, electrode, DNA double-stranded pendulum rod, the electrode, DNA double-stranded pendulum rod is set in buffer solution;Step voltage reduction signal molecule is inserted on the phosphate skeleton of the DNA double-stranded pendulum rod;One end of the DNA double-stranded pendulum rod is connected on electrode, and one single strand on the other end of the DNA double-stranded pendulum rod is connected with target aptamer.The application has wider detection range, higher sensitivity and lower detection limit, and has important significance for detecting protein, virus, nucleic acid and some small molecule substances.