13 results about "Protein virus" patented technology

The application relates to the technical field of detecting O-type foot-and-mouth disease virus antibodies, and specifically discloses a multi-epitope VP1 fusion protein of O-type foot-and-mouth disease virus, a virus-like particle, a polyclonal antibody and application of the multi-epitope VP1 fusion protein, the virus-like particle, the polyclonal antibody and the like. The application takes the virus-like particle as a competitive antigen, takes the polyclonal antibody as a labeled antibody, and establishes a method for detecting O-type foot-and-mouth disease virus antibodies based on a competition method. The method not only has the advantages of simple operation, short detection time and the like, but also covers main topological types of O-type foot-and-mouth disease virus, avoids missing detection, and has the advantages of high specificity, high sensitivity, good stability and the like.

To provide a substrate for suspension culture of adhesive cells, which is used for suspension culture of adhesive cells capable of producing a substance selected from proteins, glycoproteins, viruses, and viral vectors, wherein the substrate exhibits superior culture performance and enables further increase in the production of the substance.SOLUTION: A substrate for suspension culture of adhesive cells comprises a laminate in which a porous layer and a nonwoven fabric layer having an apparent density of less than 0.440 g / cm3 are laminated.SELECTED DRAWING: None

The invention discloses a therapeutic vaccine for human papilloma virus infection and a preparation method thereof. In particular to a composition for preventing and / or treating diseases caused by HPV infection. The composition comprises DC cells loaded with HPV antigens and exosomes. The invention also discloses a preparation method of the composition. The therapeutic vaccine provided by the invention comprises a SiHa cell exosome, an MS751 cell exosome and a human DC cell loaded with HPV VLPs (L1 protein virus-like particles of HPV). Experiments show that the therapeutic vaccine can induce and significantly enhance HPV specific CTL immune response, has an obvious killing effect on cervical cancer cells, can enhance anti-tumor immune response, and enhances the immunity of an organism to diseases, thereby achieving a therapeutic effect. The therapeutic vaccine can effectively treat HPV infection and diseases caused by the HPV infection, or prevent deterioration of the HPV infection and the diseases caused by the HPV infection.

The invention provides a porcine circovirus type 5 Cap protein, a virus-like particle, an elisa plate, an ELISA kit and application thereof, and belongs to the technical field of animal viruses. The invention provides a porcine circovirus type 5 Cap protein with an amino acid sequence as shown in SEQ ID NO. 1. The porcine circovirus type 5 Cap protein provided by the invention has the advantages of stable structure and good antigenicity, can be specifically combined with a circovirus type 5 antibody in a serum sample, and can be self-assembled into 60 virus-like particles. The ELISA plate and the ELISA kit for detecting the porcine circovirus type 5, provided by the invention, have the advantages of strong specificity and high sensitivity, and can accurately detect whether a blood sample contains the porcine circovirus type 5 or not. The porcine circovirus type 5 virus-like particle provided by the invention has the advantages of high yield, good uniformity and strong stability, and can be subsequently prepared into a porcine circovirus type 5 subunit vaccine.

The invention discloses a chicken infectious bursal disease virus VP2 protein, a VP2 protein virus-like particle vaccine and a preparation method thereof. The method comprises the following steps: firstly, optimally designing an IBDV VP2 protein; secondly, a pCold II-SUMO prokaryotic expression vector is obtained by adding SUMO protein on the basis of an escherichia coli cold shock expression vector pCold II, the prokaryotic soluble expression efficiency of the VP2 protein is improved, and the concentration of the purified VP2 protein with the SUMO label can reach 1.00 mg / mL; after the SUMO label is removed, the VP2 protein can be 100% assembled into VLPs in a specific solution, and after emulsification-demulsification, the VP2 protein still keeps a complete VLPs structure instead of a VP2 monomer, the stability is good, and experimental results show that the VLPs vaccine can 100% protect SPF chicken from IBDV virulent virus attack. Besides, the preparation method of the VP2 protein is simple, molecular sieve chromatography purification with high cost is avoided, complex processes such as ultracentrifugation are not needed, the recovery rate of two-step affinity purification is close to 60%, the purity of the purified VP2 protein is about 80%, and industrial-scale preparation of IBDV structural protein and IBDV VLPs vaccines becomes possible.

The invention belongs to the technical field of Alzheimer's disease, and particularly relates to application of a Borna disease virus type 1 virus peptide and a derivative of the Borna disease virus type 1 virus peptide to preparation of a medicine for preventing and / or treating Alzheimer's disease, the virus peptide is X protein, and the derivative of the virus peptide is X protein derived peptide PX3, the derivative PX3 of the virus peptide has a remarkable protection effect on SH-SY5Y cell injury induced by A beta, and a new strategy is provided for preventing and / or treating the Alzheimer disease.

The invention relates to a model for recruiting and concentrating a NuRD compound in a cell nucleus by using a transcription factor SALL4 and application. The construction method of the model for recruiting and concentrating the NuRD compound in the cell nucleus by using the transcription factor SALL4 comprises the following steps: 1) constructing an SALL4-fluorescent protein fusion protein coding fragment; 2) constructing an SALL4-fluorescent protein fusion protein virus expression vector; 3) packaging the virus for expressing the SALL4-fluorescent protein fusion protein; 4) performing primary isolation / in-vitro culture on target cells; and infecting the target cells in the step 4) by utilizing the virus vector constructed in the step 3), namely expressing SALL4-fluorescent protein fusion protein in the target cells to obtain the cell model for recruiting and concentrating the NuRD compound in the cell nucleus by utilizing the transcription factor SALL4. By utilizing the scheme provided by the invention, the model for recruiting and concentrating the NuRD compound in the cell nucleus can be established. The model provided by the invention can be applied to intracellular NuRD in-situ electron microscope structure analysis.

The application discloses a kind of electrochemical biomolecular sensor and construction method based on DNA molecular pendulum, including buffer solution, electrode, DNA double-stranded pendulum rod, the electrode, DNA double-stranded pendulum rod is set in buffer solution;Step voltage reduction signal molecule is inserted on the phosphate skeleton of the DNA double-stranded pendulum rod;One end of the DNA double-stranded pendulum rod is connected on electrode, and one single strand on the other end of the DNA double-stranded pendulum rod is connected with target aptamer.The application has wider detection range, higher sensitivity and lower detection limit, and has important significance for detecting protein, virus, nucleic acid and some small molecule substances.

The application provides a purification method of foot-and-mouth disease virus capsid protein virus-like particles, and the purification method comprises the following steps: a fusion protein containing P1-2A is subjected to enzymolysis by 3C and TEV proteases to obtain an enzymolysis liquid; the enzymolysis liquid is subjected to nickel column purification by a nickel column to obtain foot-and-mouth disease virus capsid protein virus-like particle 5S protomers; and then foot-and-mouth disease virus capsid protein virus-like particles VLPs can be obtained by self-assembly. The purity of the foot-and-mouth disease virus capsid protein virus-like particles obtained by the purification method of the application is more than 90%, and the yield is more than 95%.

This invention obtains the porcine circovirus type 2 (PCV2Cap) protein through mutation, and expresses it using a yeast expression system to obtain PCV2Cap protein with good solubility and immunogenicity, and significantly increased expression levels. The Cap protein constructed in this invention can be used in the production of subunit vaccines and VLPs vaccines, offering advantages such as good immunogenicity and low cost.

The present application relates to a model for recruiting and concentrating NuRD complex in the nucleus by transcription factor SALL4 and application. The method for constructing the model for recruiting and concentrating NuRD complex in the nucleus by transcription factor SALL4 comprises the following steps: 1) constructing a SALL4-fluorescent protein fusion protein coding fragment; 2) constructing a SALL4-fluorescent protein fusion protein virus expression vector; 3) packaging a virus for expressing SALL4-fluorescent protein fusion protein; 4) primary separation / in vitro culture of target cells; the target cells in 4) are infected by the virus vector constructed in 3), that is, SALL4-fluorescent protein fusion protein is expressed in the target cells, and a cell model for recruiting and concentrating NuRD complex in the nucleus by transcription factor SALL4 is obtained. According to the scheme provided in the present application, a model for recruiting and concentrating NuRD complex in the nucleus can be established. The model provided in the present application can be applied to in situ electron microscope structure analysis of NuRD in cells.

The invention discloses a therapeutic vaccine for human papilloma virus infection and a preparation method thereof. In particular to a composition for preventing and / or treating diseases caused by HPV infection. The composition comprises DC cells loaded with HPV antigens and exosomes. The invention also discloses a preparation method of the composition. The therapeutic vaccine provided by the invention comprises a SiHa cell exosome, an MS751 cell exosome and a human DC cell loaded with HPV VLPs (L1 protein virus-like particles of HPV). Experiments show that the therapeutic vaccine can induce and significantly enhance HPV specific CTL immune response, has an obvious killing effect on cervical cancer cells, can enhance anti-tumor immune response, and enhances the immunity of an organism to diseases, thereby achieving a therapeutic effect. The therapeutic vaccine can effectively treat HPV infection and diseases caused by the HPV infection, or prevent deterioration of the HPV infection and the diseases caused by the HPV infection.

The invention relates to the technical field of veterinary biological products, and particularly provides a porcine circovirus type 3 Cap protein and a porcine circovirus type 3 subunit vaccine. The amino acid in the NLS region of the Cap protein of the porcine circovirus type 3 (PCV3) has 12R-K substitution, and 75-76aa (AI) is substituted into PGGGSNPR. The amino acid sequence of the Cap protein is as shown in SEQ ID NO. 4. The invention also provides a PCV3 virus-like particle antigen which is the Cap protein of the porcine circovirus type 3 (PCV3). The virus-like particle vaccine comprises an immunizing dose of the PCV3 virus-like particle antigen and a pharmaceutically acceptable carrier. According to the invention, the correct assembly of the PCV3 virus-like particles is promoted, and the stability of the protein is improved.