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Porcine circovirus type 2 (PCV2) Cap protein virus-like particle (VLP) preservation solution and application

A porcine circovirus, protein virus technology, applied in the direction of virus, antiviral agent, virus antigen component, etc., can solve the problems of difficult preservation, poor stability of preservation solution, etc.

Inactive Publication Date: 2021-01-22
BEIJING KEMUFENG BIOLOGICAL PHARMA +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, the technical problem to be solved by the present invention is to provide a kind of porcine circovirus type 2 Cap protein virus-like particle preservation solution formula, to solve the poor preservation solution stability of Cap protein in the prior art, and cause the difficult problem of preservation ;

Method used

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  • Porcine circovirus type 2 (PCV2) Cap protein virus-like particle (VLP) preservation solution and application
  • Porcine circovirus type 2 (PCV2) Cap protein virus-like particle (VLP) preservation solution and application
  • Porcine circovirus type 2 (PCV2) Cap protein virus-like particle (VLP) preservation solution and application

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Embodiment 1

[0044] The configuration of porcine circovirus type 2 Cap protein virus-like particle preservation solution described in this embodiment comprises the following steps:

[0045] (1) Prepare 20mM / ml Tris-HCI buffer solution for later use; based on the Tris-HCI buffer solution, prepare the following components: 1wt% sucrose, 1wt% trehalose, 0.1wt% Tween-20, EDTA concentration 2mM, glycerin 5wt%, gelatin 0.1wt%, lysine 0.5wt%, arginine 0.5wt%, sorbic acid 0.001wt%, spare;

[0046](2) Add the sugars, non-ionic surfactants, metal ion chelating agents, alcohols, protein stabilizers, amino acids and preservatives to the above buffer in sequence, mix at 500rpm / ml for 10min, then add at a concentration of 200ug / ml porcine circovirus type 2 Cap protein, the volume ratio of the volume ratio of the porcine circovirus type 2 Cap protein to control the preservation solution is 15v / v%, 1000rpm / ml mixed for 10min, and put Store at 2-8°C or -20°C.

Embodiment 2

[0048] The configuration of porcine circovirus type 2 Cap protein virus-like particle preservation solution described in this embodiment comprises the following steps:

[0049] (1) Prepare 20mM / ml Tris-HCI buffer solution for later use; based on the Tris-HCI buffer solution, prepare the following components: 1wt% sucrose, 1wt% trehalose, 0.1wt% Tween-20, EDTA concentration 1mM, mannitol 5wt%, gelatin 0.1wt%, lysine 0.5wt%, arginine 0.5wt%, sodium diacetate 0.005wt%, spare;

[0050] (2) Add the sugars, non-ionic surfactants, metal ion chelating agents, alcohols, protein stabilizers, amino acids and preservatives to the above buffer in sequence, mix at 500rpm / ml for 10min, then add at a concentration of The porcine circovirus type 2 Cap protein of 20ug / ml, the usage amount of control described preservation solution accounts for described porcine circovirus type 2 Cap protein volume ratio is 5v / v%, 1000rpm / ml mixes 10min, and puts Store at 2-8°C or -20°C.

Embodiment 3

[0052] The configuration of porcine circovirus type 2 Cap protein virus-like particle preservation solution described in this embodiment comprises the following steps:

[0053] (1) Prepare 100mM / ml Tris-HCI buffer solution for later use; based on the Tris-HCI buffer solution, prepare the following components: 2wt% sucrose, 3wt% trehalose, 0.5wt% Tween-80, EDTA concentration 5mM, sorbitol 10wt%, gelatin 1.5wt%, lysine 0.5wt%, sodium diacetate 0.005wt%, spare;

[0054] (2) Add the sugars, non-ionic surfactants, metal ion chelating agents, alcohols, protein stabilizers, amino acids and preservatives to the above buffer in sequence, mix at 500rpm / ml for 10min, then add at a concentration of 3mg / ml of porcine circovirus type 2 Cap protein, the volume ratio of the volume ratio of the porcine circovirus type 2 Cap protein to control the amount of the preservation solution is 15v / v%, 1000rpm / ml mixed for 10min, and put Store at 2-8°C or -20°C.

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Abstract

A porcine circovirus type 2 (PCV2) Cap protein virus-like particle (VLP) preservation solution is based on a salt ion buffer solution system, saccharides, a nonionic surfactant, a metal ion chelatingagent, alcohols, a protein stabilizer, amino acids and a preservative are added, the PCV2 Cap protein VLP preservation solution can prolong the preservation period of the PCV2 Cap protein effectively,preserve the PVC2 Cap protein for 12 months at 2-8 DEG C while keeping protein concentration and activity unchanged, preserve the PCV2 Cap protein for 36 months at subzero 20 DEG C while keeping protein concentration and activity unchanged, can be used as a preservation solution of a standard protein of a kit for detecting the PCV antigen concentration or a diluted solution of a PCV2 Cap proteinsubunit vaccine.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnostic reagents, in particular to a formula of porcine circovirus type 2 Cap protein virus-like particle preservation solution, and further discloses its application. Background technique [0002] Porcine circovirus (PCV) is one of the smallest animal viruses found so far. It is known that PCV has two serotypes, namely PCV1 and PCV2. PCV1 is a non-pathogenic virus and PCV2 is pathogenic Virus. Among them, pigs have strong susceptibility to PCV2. Infected pigs can excrete virus from nasal fluid, feces and other wastes, and infect pigs of different ages through oral and respiratory routes. PCV2 is the primary pathogenic factor of multisystem failure syndrome, dermatitis and nephrotic syndrome and other related diseases in weaned piglets, causing huge economic losses to the pig industry. According to reports, porcine circovirus type 2 (PCV2) is widely distributed around the world. Serological ...

Claims

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Application Information

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IPC IPC(8): A61K39/12A61K47/26A61K47/18A61K47/42A61K47/12A61P31/20G01N33/569
CPCA61K39/12A61K47/12A61K47/183A61K47/26A61K47/42A61K2039/552A61P31/20C12N2750/10034G01N33/56983G01N2333/01G01N2469/10
Inventor 于萍萍张渊魁王敏刘奇徐慧敏卢晓楠刘飞汤波韩昱鹏吕帅阳聂思静刘运平
Owner BEIJING KEMUFENG BIOLOGICAL PHARMA
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