Method for transfecting cells through ultrasonic perforation

A technology of transfection cells and ultrasound, which is applied in the field of cell transfection, can solve the problems of high cell death rate, virus risk restricting large-scale use, cell damage, etc., and achieves simple operation, reduced professional skill requirements and time investment requirements , the effect of cost reduction
CN112266930AActive Publication Date: 2021-01-26SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Publication Date
2021-01-26

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Abstract

The invention discloses a method for transfecting cells through ultrasonic perforation. The method comprises the following steps: firstly, culturing target cells; secondly, mixing the target cells, atarget object and an ultrasonic contrast agent in a container; thirdly, applying ultrasonic waves to the container; and fourthly, re-culturing the container under a constant temperature condition, sothat the target object fully enters the target cells, wherein the target object is a target gene or a target protein, and when the target object is the target gene, the frequency of the ultrasonic waves adopted in the third step is 800-900 kHz; and when the target object is the target protein, the frequency of the ultrasonic waves adopted in the third step is 800-1200 kHz. The method for transfecting cells through ultrasonic perforation is simple to operate, high in transfection efficiency and low in cost, and can be used for converting or transfecting molecules such as DNA (Deoxyribonucleic Acid), RNA (Ribose Nucleic Acid), proteins, viruses, sugar, medicines and nanoparticles in an unlimited range.
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Description

technical field

[0001] The invention relates to the technical field of cell transfection, in particular to a method for ultrasonically transfecting cells. Background technique

[0002] Cell engineering, genetic engineering, genetic engineering and other disciplines or industries all involve eukaryotic cells (such as bacteria) and prokaryotic cells (such as fungi, mice, sheep, human cells), etc. Transport of viruses, proteins, drugs, nanomaterials, etc. from extracellular to intracellular. In the field of life science research, this process is a necessary process for cells to obtain new genetic traits or protein expression; in the development of new drugs, this process is applied to small batch protein / antibody preparation, antibody drug screening, and positive clone screening; In immunotherapy, it is also a key technical link in the transient preparation of CAR-T, CAR-NK and other cells. Therefore, the transport of molecules into cells involves the whole industry of life s...

Claims

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