IBV-III antigen production process

Abstract

The invention supplies a manufacturing technique of IBV-HI antigen, the preparation process of antigen is that: the virus fluid of containing the protein virus is isolated from chicken embryo allantois fluid of containing respiratory type IBV the virus and blending with clostridium filtrate of rabbits A type wei surname as a culture medium, after two hours water bath action with 37deg.C, put the fluid into the 4deg.C fridge for 48 hours, through freeze drying to get antigen, the related centrifuge divided into two centrifugal, first the mixed fluid through high speed centrifugation, choose the supernatant after the centrifugation; then it progress the two centrifugation as ultracentrifugation, the time is 2-3h, the rotation rate of centrifugation is 25000-35000g, abandon the supernatant after the centrifugation, and use deposit suspension to obtain virus fluid. Using this invention method, infectious bronchitis HI antigen of respiratory system has relatively low cost, type specificity, high sensitivity, stability feature, it makes HI method to detect respiratory infectious bronchitis feasible, and enhance the credibility and maneuverability of its detection.

Description

technical field [0001] The invention relates to biotechnology, in particular to a production process of IBV-HI (respiratory infectious bronchitis) antigen. Background technique [0002] Chicken infectious bronchitis (Infectious Bronchitis, referred to as IB) is caused by infectious bronchitis virus (Infectious Bronchitis Virus, referred to as IBV) as an acute, highly contagious respiratory and genitourinary disease in chickens, characterized by tracheal rales Respiratory symptoms such as coughing, sneezing, kidney swelling, and uric acid deposition in the ureter are the main pathological features, and can also cause swelling of the glandular stomach, enteritis, oviduct cyst, and atrophy of chickens, which are also important causes of "false hens". reason. There are more than 20 serotypes of IBV, and there is no or only partial cross-protection between different serotypes. Respiratory infectious bronchitis is currently the main virus strain in chicken flocks in my country, ...

AI Technical Summary

Problems solved by technology

IBV treated with trypsin, although it has hemagglutination activity, is non-specific and is not suitable as a detection antigen; the potency of IBV-HI antigen prepared by PLCI is the same as that prepared by using rabbit Clostridium welchii type A culture fluid The IBV-HI antigen is equivalent, but due to the lack of synergistic effect of other factors in the culture solution of Clostridium welchii type A in rabbits, the production cost is relatively high, which affects the popularization and application of the antigen

Therefore, it is not practical to produce and use it as an antigen for detection, and generally it can only be used as a laboratory test.

However, the cost of culture solution of Clostridium welchii type A in rabbits is low, but when the current technology is used for production, the entire production process takes a long time, and the final antigen has a short validity period, which cannot achieve the purpose of long-term detection and cannot Carry out mass production and practical application

At the same time, in the prior art, the method of dialysis is generally used to concentrate the virus liquid. With this method, the dialysis concentration takes a long time and only a small amount of virus liquid can be concentrated, and it cannot be concentrated in large quantities and in a long time The operation of time will have a certain impact on the virus itself, inactivating part of the virus and reducing the titer of the prepared antigen. Therefore, this method can only be used as a laboratory test and cannot be used in batch production.