Reagent kit for measuring serum amyloid A (SAA) concentration, and preparation method for reagent kit for measuring SAA concentration

A serum starch and kit technology, applied in the biological field, can solve problems such as poor sensitivity and linear range, and achieve the effect of improving repeatability and sensitivity

Inactive Publication Date: 2019-01-11
苏州普瑞斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conventional methods, latex microspheres are used to directly couple SAA antibodies. Our company found that the sensitivity and linear range of this method are poor

Method used

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  • Reagent kit for measuring serum amyloid A (SAA) concentration, and preparation method for reagent kit for measuring SAA concentration
  • Reagent kit for measuring serum amyloid A (SAA) concentration, and preparation method for reagent kit for measuring SAA concentration
  • Reagent kit for measuring serum amyloid A (SAA) concentration, and preparation method for reagent kit for measuring SAA concentration

Examples

Experimental program
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Effect test

Embodiment 1

[0042] A test kit for measuring the concentration of serum amyloid A, comprising reagent R1 and reagent R2, each component and concentration of the reagent R1 comprising: as the first buffer A dihydrate sodium dihydrogen phosphate, its concentration is 0.8g / L, as the first buffer B, disodium hydrogen phosphate dodecahydrate, its concentration is 24.31g / L, as the first electrolyte sodium chloride, its concentration is 5.8g / L, as the first polymer PEG 6000 as accelerator, its concentration is 40g / L, and Proclin-950 as the first preservative, its concentration is 2.0%.

[0043]The reagent R2 includes a second buffer, a preservation solution, latex microspheres coupled with SAA polyclonal antibody-biotin-streptavidin, and the second buffer is 2-(N-morpholine) B Sulfonic acid monohydrate, its concentration is 8.05g / L; Described preservation solution comprises: concentration is the N,N-bicine of 9.83g / L, the bovine serum albumin as stabilizer, its concentration is 0.5 g / L, Proclin-...

Embodiment 2

[0055] A test kit for measuring the concentration of serum amyloid A, comprising reagent R1 and reagent R2, each component and concentration of said reagent R1 includes sodium dihydrogen phosphate dihydrate as the first buffer A, and its concentration is 3.2 g / L, as the disodium hydrogen phosphate dodecahydrate of the first buffer solution B, its concentration is 8.75g / L, as the sodium chloride of the first electrolyte, its concentration is 20.0g / L, as the first polymer promoter The PEG 6000 of reagent, its concentration is 80g / L, the Proclin-300 as the first preservative, its concentration is 0.8%, described reagent R2 includes the second buffer solution, storage solution, is coupled with SAA polyclonal antibody-biological The latex microsphere of prime-streptavidin, described second buffer solution is 2-(N-morpholine) ethanesulfonic acid monohydrate, and its concentration is 27.30g / L; Described preservation solution comprises: concentration is 5.0g / L of N,N-bishydroxyethylgl...

Embodiment 3

[0067] A test kit for measuring the concentration of serum amyloid A, comprising reagent R1 and reagent R2, each component and concentration of said reagent R1 includes sodium dihydrogen phosphate dihydrate as the first buffer A, and its concentration is 2g / L, disodium hydrogen phosphate dodecahydrate as the first buffer solution B, its concentration is 16g / L, sodium chloride as the first electrolyte, its concentration is 12g / L, PEG as the first polymer accelerator 6000, its concentration is 120g / L, as the thimerosal of the first preservative, its concentration is 1.0%, and described reagent R2 comprises second buffer solution, preservation solution, is coupled with SAA polyclonal antibody-biotin-streptavidin For the latex microspheres of Hesu, the second buffer is 2-(N-morpholine) ethanesulfonic acid monohydrate, and its concentration is 16.80g / L; the preservation solution includes: concentration of 1.26g / L N,N-bishydroxyethylglycine, mannitol as a stabilizer, its concentrat...

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Abstract

The invention relates to the technical field of biology, and specifically relates to a reagent kit for measuring a serum amyloid A (SAA) concentration, and a preparation method for the reagent kit formeasuring the SAA concentration. The invention applies the following technical solution: a reagent kit for measuring an SAA concentration is characterized in that the reagent kit for measuring the SAA concentration includes a reagent R1 and a reagent R2, the reagent R1 includes a buffer solution, an accelerator and a preservative, the reagent R2 includes a buffer solution, a stabilizer, a latex microsphere to which SAA polyclonal antibody-biotin-streptavidin is coupled, and a preservative. The reagent kit for measuring the SAA concentration has the following advantage: the reagent kit for measuring the SAA concentration of the invention applies the latex microsphere connected with the streptavidin in a ratio of 1:1, and the SAA antibody connected with the biotin in a ratio of 1:2, and ina ratio of 1:1, the latex microsphere connected with the streptavidin is further mixed and reacted with the SAA antibody connected with the biotin, thus the repeatability and sensibility can be improved evidently and the linear range can achieve 5-400 mg / L.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and a preparation method for measuring the concentration of serum amyloid A. Background technique [0002] Serum amyloid A (serum amyloid A, SAA) is the precursor of tissue amyloid A, which belongs to the acute phase protein. It increases during tissue injury and inflammatory response, and affects cell adhesion, migration, proliferation and aggregation. SAA is the serum precursor of amyloid A protein, the main component of amyloidosis. It is an acute phase protein that is secreted into serum after being produced by liver cells. Although it can be seen everywhere, under normal circumstances, in the body The expression is still very low, but once inflammation, infection or tissue damage occurs in the body, the level of SAA can rapidly increase by about 1000 times within 5-6 hours. CRP is an acute phase response protein that has been studied more and widely used clinically. Howev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 袁嘉扬吴朝晖杜微微李磊
Owner 苏州普瑞斯生物科技有限公司
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