Method of separating and purifying dihydromyricetin from ampelopsis grossedentata

A technology for purifying dihydromyricetin and vine tea, which is applied in the direction of organic chemistry and can solve the problems of reduced yield and low purity of dihydromyricetin

Inactive Publication Date: 2019-02-05
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented process involves removing impurities like flavonoids or other compounds found on green teas that make them taste better when consumed without adding any extra ingredients such as sugar. By carrying out these processes twice before collecting pure samples, high-purity products may be achieved while also increasing yields compared to traditional methods.

Problems solved by technology

The technical problem addressed by this patented technology relates to finding an improved way to make highly pure or more effective sources of dihyodermicillins like DHA which have beneficial properties including reducing harmful chemical reactions caused by excess consumption during food processing processes, protecting against oxidative stress, treatments related diseases, improve immune function, suppress inflammations, act on bone tissue growth factors, enhances cellular activity, stimulate stem cells, promote healing wounds, increase resistance towards cancer development, aid digestion, decrease cholesterols levels, help fight off cardiofibrils associated with heart attacks, and provide medicines useful therefor.

Method used

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  • Method of separating and purifying dihydromyricetin from ampelopsis grossedentata
  • Method of separating and purifying dihydromyricetin from ampelopsis grossedentata
  • Method of separating and purifying dihydromyricetin from ampelopsis grossedentata

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Embodiment 1

[0041] This embodiment provides a kind of dihydromyricetin, which is prepared by adopting the following method of extracting dihydromyricetin from vine tea, and its specific process is as follows:

[0042] S11, crush the rattan tea and pass it through a 40-mesh sieve, and collect the sifted rattan tea powder.

[0043] S12. Take 100g of the above vine tea powder and add 2000ml of water to mix evenly, then stir and extract at 90°C for 1h, and centrifuge to obtain a water extract, such as figure 1 shown.

[0044] S13. Adsorb the water extract with D-101 macroporous resin, heat it at 45°C for 1.5h and then cool it to 25°C, then wash the macroporous resin twice with water three times the volume of the macroporous resin, and then use macroporous resin The mass percentage of 3 times of the volume of the porous resin is 40% ethanol solution to wash the macroporous resin 2 times, and finally the macroporous resin is washed with 90% ethanol solution of 3 times of the mass percentage of t...

Embodiment 2

[0050] This embodiment provides a kind of dihydromyricetin, which is prepared by adopting the following method of extracting dihydromyricetin from vine tea, and its specific process is as follows:

[0051] S21, crush the rattan tea and pass it through a 50-mesh sieve, and collect the sifted rattan tea powder.

[0052] S22. Take 100 g of rattan tea powder and 2200 ml of water and mix evenly, then stir and cold-extract at 85° C. for 1.5 h, and filter to obtain a water extract.

[0053] S23. Use the macroporous resin to adsorb the water extract, adsorb at 40°C for 2h, then cool to 30°C, then wash the macroporous resin twice with water twice the volume of the macroporous resin, and then use twice the volume of the macroporous resin Wash the macroporous resin twice with 40% ethanol solution by mass percentage, and finally wash the macroporous resin with 90% ethanol solution with 4 times the mass percentage of the macroporous resin, and collect 90% ethanol washing solution to obtain...

Embodiment 3

[0058] This embodiment provides a kind of dihydromyricetin, which is prepared by adopting the following method of extracting dihydromyricetin from vine tea, and its specific process is as follows:

[0059] S31, crush the rattan tea and pass it through a 60-mesh sieve, and collect the sifted rattan tea powder.

[0060] S32. Take 100 g of rattan tea powder and 2400 ml of water and mix evenly, then stir and condense at 80° C. for reflux for 1 hour, and filter to obtain a water extract.

[0061] S33. Use the macroporous resin to adsorb the water extract, adsorb at 50° C. for 1 hour, then cool to 28° C., then wash the macroporous resin once with water 3 times the volume of the macroporous resin, and then use 3 times the volume of the macroporous resin Wash the macroporous resin once with 35% ethanol solution by mass percentage, and finally wash the macroporous resin with 80% ethanol solution 5 times the mass percentage of the macroporous resin, and collect the 80% ethanol washing s...

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Abstract

The invention discloses a method of separating and purifying dihydromyricetin from ampelopsis grossedentata and belongs to the field of organic compound separation. The method of separating and purifying the dihydromyricetin from the ampelopsis grossedentata comprises the following steps of acquiring a water extract of the ampelopsis grossedentata; performing adsorption on the water extract by using macroporous resin, then sequentially washing the macroporous resin with water and an ethanol solution with the mass percentage being 30-50 percent, and then washing the macroporous resin with an ethanol solution with the mass percentage being 70-90 percent to obtain an eluent; and performing concentration and crystallization on the eluent, and performing washing and drying. By adopting the method of separating and purifying the dihydromyricetin from ampelopsis grossedentata, the dihydromyricetin with the purity being 98 percent or above can be obtained, and the yield of the dihydromyricetincan reach 60 percent or above.

Description

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Claims

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Application Information

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Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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