Method for inferring activity of a transcription factor of a signal transduction pathway in a subject

A technology of transcription factor and signal transduction, applied in the field of evaluating the therapeutic suitability of the subject, which can solve the problem of insufficient specificity of activity

Pending Publication Date: 2019-02-05
KONINKLJIJKE PHILIPS NV
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  • Claims
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Problems solved by technology

A possible reason for this high non-response rate may be that in individual patients, despite positive ER staining results, ER signaling pathways may actually be inactive, suggesting that ER staining is important in assessing ER signaling pathways in patients Not specific enough in terms of activity

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  • Method for inferring activity of a transcription factor of a signal transduction pathway in a subject
  • Method for inferring activity of a transcription factor of a signal transduction pathway in a subject
  • Method for inferring activity of a transcription factor of a signal transduction pathway in a subject

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Embodiment Construction

[0064] In the drawings, the same or corresponding reference signs indicate the same or corresponding parts and / or elements.

[0065] In the following, we will refer to figure 1 The flowchart shown in exemplarily describes the first embodiment of a method for inferring the activity of transcription factors of a signal transduction pathway (here, ER-α signal transduction pathway) in a subject. Here, the subject is a medical subject, particularly a cancer patient, more particularly a breast cancer patient.

[0066] In step 101, a first staining is performed to detect transcription factors (here, ER-α) in cells in a sample (in this example, breast cancer patient tissue). In step 102, a second staining is performed to detect the protein (here, PR) encoded by the target gene (here, PgR) of the transcription factor (ie, ER-α) in the cells in the sample. The first staining and the second staining are performed on the same slice of the sample. Here, both the first staining and the second ...

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Abstract

The present invention relates to a method for inferring activity of a transcription factor of a signal transduction pathway in a subject. The method comprises performing (101) a first staining for detecting the transcription factor in cells of a sample of the subject and performing (102) a second staining for detecting a protein encoded by a target gene of the transcription factor in cells of thesample. The method further comprises quantifying (103) cells of the sample that show both a nuclear presence of the transcription factor and a presence of the target gene-encoded protein based on thefirst staining and the second staining, and inferring (104) the activity of the transcription factor in the subject based on the quantifying. This allows the presented method to more reliably infer the activity of the transcription factor in the subject.

Description

Technical field [0001] The present invention relates to a method for inferring the activity of transcription factors of a signal transduction pathway in a subject. The present invention also relates to a method for stratifying a subject and a method for assessing the suitability of a subject for treatment, the treatment involving a signal transduction pathway, wherein the method includes a method for inferring the signal transduction pathway in the subject Method of transcription factor activity. Background technique [0002] Conventional pathological stains in breast cancer today include ER (estrogen receptor), PR (progesterone receptor) and HER-2 (human epidermal growth factor receptor 2) staining, for example, by performing immunohistochemistry (IHC) Measurement, in which, usually, staining is performed once per slice. According to clinical diagnostic guidelines, if more than 1% to 10% of cancer cells in the sample express nuclear ER and PR, respectively, ER and PR are consi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G16B25/00G16B99/00G16B25/10
CPCG01N33/574G01N2800/52G01N33/57415G16B25/10G16B5/20G01N33/6872G01N33/6875G01N2333/723
Inventor A·范德斯托尔佩F·范赫默特W·F·J·费尔哈格R·温贝格尔-弗里德尔J·C·P·范德芬
Owner KONINKLJIJKE PHILIPS NV
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