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Medicament capable of controllpreventing and treating fresh water ichthyophthiriasis for cage culture

A small melon worm disease and net cage culture technology, which is applied in the direction of anti-infective drugs, drug combinations, medical formulas, etc., can solve the problems of long production time of Chinese herbal medicine preparations, easy delay of treatment timing, and ineffective effects, so as to reduce damage The risk of host tissue, the prevention of melon worm disease, the effect of more exposure opportunities

Inactive Publication Date: 2019-02-12
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of simple chemical drugs and Chinese herbal medicines is relatively large in the flowing water environment of cages, which will not only pollute the environment, but also have high price and cost, and the effect is not obvious. delayed treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method for an immune enhancer for preventing and treating freshwater cucurbit disease, comprising the steps of:

[0032] S1: Dissolve lecithin and cholesterol with a weight ratio of 1:0.12 in an equal volume of ethanol and chloroform mixture, sonicate for 1 minute to make the material completely dissolved and mix uniformly and stably, then pour it into a container, and use a rotating method at 45°C The evaporator evaporates the dispersion to remove the organic solvent and form a lipid film;

[0033] S2: Microwave the drug solution and lipid film until the lipid film is completely hydrated and dissolved to form a uniform emulsion. The microwave treatment adopts intermittent heating with heating for 30s and pause for 60s. The microwave treatment power is 300W and the temperature is 30°C. The time is 8 minutes;

[0034] S3: Ultrasonic treatment of the emulsion to obtain a liposome emulsion, the ultrasonic treatment adopts the mode of ultrasonic treatment for...

Embodiment 2

[0043] A preparation method for an immune enhancer for preventing and treating freshwater cucurbit disease, comprising the steps of:

[0044] S1: Dissolve lecithin and cholesterol with a weight ratio of 1:0.125 in an equal volume of ethanol and chloroform mixture, sonicate for 1 minute to make the material completely dissolved and mix uniformly and stably, then pour it into a container, and use a rotating method at 45°C The evaporator evaporates the dispersion to remove the organic solvent and form a lipid film;

[0045] S2: Microwave the drug solution and lipid film until the lipid film is completely hydrated and dissolved to form a uniform emulsion. The microwave treatment adopts intermittent heating with heating for 30s and pause for 60s. The microwave treatment power is 300-500W and the temperature is 35 ℃, the time is 10min;

[0046] S3: Ultrasonic treatment of the emulsion to obtain a liposome emulsion, the ultrasonic treatment adopts the mode of ultrasonic treatment fo...

Embodiment 3

[0052] A preparation method for an immune enhancer for preventing and treating freshwater cucurbit disease, comprising the steps of:

[0053] S1: Dissolve lecithin and cholesterol with a weight ratio of 1:0.13 in an equal volume of ethanol and chloroform mixture, sonicate for 1 minute to make the material completely dissolved and mix uniformly and stably, then pour it into a container, and use a rotating method at 45°C The evaporator evaporates the dispersion to remove the organic solvent and form a lipid film;

[0054] S2: Microwave the drug solution and lipid film until the lipid film is completely hydrated and dissolved to form a uniform emulsion. The microwave treatment adopts intermittent heating with heating for 30s and pause for 60s. The microwave treatment power is 500W and the temperature is 38°C. The time is 12 minutes;

[0055] S3: Ultrasonic treatment of the emulsion to obtain a liposome emulsion, the ultrasonic treatment adopts the mode of ultrasonic treatment fo...

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PUM

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Abstract

The invention provides a medicament capable of preventing and treatingcontrolling fresh water ichthyophthiriasis for cage culture, and belongs to the technical field of aquaculture. A preparation method for an immunopotentiator for the medicament comprises the following steps: evaporating an organic solvent from dispersion liquid containing lecithin and cholesterol to form a lipid membrane; performing microwave treatment on a medicine solution and the lipid membrane tountil the lipid membrane is completely hydrated, and dissolving to form uniform emulsion; performing ultrasonic treatment on the emulsion to obtain lipidosome emulsion; enabling the lipidosome emulsion to sequentially pass through a micro-pore filter membranes with different pore diameters, thereby obtaining the immunopotentiator,; wherein the microwave treatment adopts a batch type heating way of heating for 30 seconds and pausing for 60 seconds, and microwave treatment power is 300-500 W. The medicament capable of preventing and treating fresh water ichthyophthiriasis has manya high exposure toplurality ofopportunities to be in contact with ichthyophthiriasis, so that the risk that fish is infected by larvas and thehost tissue is damaged by imagoes is lowered; and meanwhile, a condition that cultured fishes are infected by other bacteria adhered on a body surface or in gills after ichthyophthiriasis occurs is avoided, and therefore, the purpose of preventing and treatingthecontrolling ichthyophthiriasis is achieved.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a medicament for preventing and treating freshwater melon worm disease in cage culture. Background technique [0002] Aquatic animals play an integral role in human life. With the continuous development of the degree of intensification of aquatic products, aquatic animal diseases are characterized by multiple, complex and high hazards, threatening the healthy development of the aquaculture industry at all times and hitting the confidence of farmers in farming. The deterioration of the breeding water environment has also led to Decreased immune levels in fish. Various food safety issues that have been exposed in recent years have also sounded the alarm for the safety of aquatic products. The healthy growth of fish and effective fish disease prevention and control measures have become the key and difficult issues that people from all walks of life in the aquacultur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K45/06A61P37/04A61P33/14A61K31/198A61K31/357A61K31/465A61K31/4706A61K31/7048A61K31/715A61K38/17
CPCA61K9/0095A61K9/1277A61K31/198A61K31/357A61K31/465A61K31/4706A61K31/7048A61K31/715A61K38/1709A61K45/06A61P33/14A61P37/04A61K2300/00
Inventor 魏可张建设周真真
Owner ZHEJIANG OCEAN UNIV
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