Preservation method of denitrifying bacteria

A technology of denitrifying bacteria and preservation method, applied in the direction of preservation of microorganisms, etc., can solve problems such as difficulty in meeting large-scale production requirements and low efficiency, and achieve the effects of reducing metabolic rate and prolonging preservation time.

Pending Publication Date: 2019-02-12
HUATIAN ENG & TECH CORP MCC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the effect of dry storage is better, it requires specific drying equipment, an

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 100mL of the denitrifying bacteria liquid independently isolated and cultivated in the laboratory (bacterial quantity: 2.6×10 9 cfu / ml, using the plate counting method, the same below), put it into a 5L square small-mouth plastic bucket for storage, add 4.9L of phosphate buffer, add FeSO 4 , MgCl 2 , MnCl 2 0.02g each and carry out uninterrupted aeration to maintain the dissolved oxygen above 5mg / L. The formula of phosphate buffer is: 0.06mol / L Na 2 HPO 4 Add 1.9L, 0.06mol / L of KH 2 PO 4 Add 3L. The mixture was stored at room temperature. After 2 weeks and 1 month of preservation, the preserved bacterial liquids were respectively taken and spread on a flat plate, and the statistical survival rates were 97.5% and 94.7%, respectively.

Embodiment 2

[0025] Take 5L of the denitrifying bacteria liquid independently isolated and cultivated in the laboratory (bacteria quantity: 2.6×10 9 cfu / ml), put into a 5L square small-mouth plastic barrel for storage, add 4.9L of phosphate buffer, and add FeCl 2 , MgSO 4 , MnCl 2 0.02g each, and carry out uninterrupted aeration to maintain its dissolved oxygen above 4mg / L. The formula of phosphate buffer is: 0.06mol / L Na 2 HPO 4 Add 1L, 0.06mol / L of KH 2 PO 4 Add 3.9L. The mixture was stored at room temperature. After 2 weeks and 1 month of preservation, the preserved bacterial liquids were respectively taken and spread on a flat plate, and the statistical survival rates were 94.1% and 89.0%, respectively.

Embodiment 3

[0027] Take 5L of the denitrifying bacteria liquid independently isolated and cultivated in the laboratory (bacteria quantity: 2.6×10 9 cfu / ml), put into a 5L square small mouth plastic bucket for storage, add 4.9L KH 2 PO 4 -NaOH buffer with addition of FeSO 4 , MgSO 4 , MnCl 2 0.05g each and carry out uninterrupted aeration to maintain the dissolved oxygen above 8mg / L. K H 2 PO 4 -NaOH buffer formula is: K of 0.03mol / L 2 HPO 4 Add 4.4 L, and add 0.5 L of 0.03 mol / L NaOH. The mixture was stored at room temperature. After 2 weeks and 1 month of preservation, the preserved bacterial liquids were respectively taken and spread on a flat plate, and the statistical survival rates were 82.6% and 78.5%, respectively.

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PUM

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Abstract

The invention discloses a preservation method of denitrifying bacteria. The method comprises the following steps: (1) collecting denitrifying bacterium thalli obtained through culture; (2) preparing preservative fluid, wherein the pH range of the preservative fluid is 5.5 to 7, and the dissolved oxygen is maintained to 4mg/L to saturated dissolved oxygen; (3) mixing the thalli obtained in the step(1) and the preservative fluid obtained in the step (2) in a preservation vessel, and carrying out preservation in a room-temperature environment. Compared with the prior art, the method disclosed bythe invention is satisfactory for long-term preservation of a large number of denitrifying bacteria and has the advantages of good normal-temperature preservation effect, high survival rate, no hazardous and noxious substances, simple formula, easiness in popularization and the like.

Description

technical field [0001] The invention relates to a method for preserving bacteria agents, in particular to a method for preserving denitrifying bacteria which is simple, effective and suitable for mass use. Background technique [0002] Excessive total nitrogen concentration will cause eutrophication of the water body, resulting in excessive algae reproduction and endangering the ecology. Since 2018, the state has implemented control over the total nitrogen in sewage plant discharges, and has higher requirements for the removal of total nitrogen. The removal of total nitrogen in the environment is mainly through the action of microorganisms. Ammonia nitrogen is oxidized to nitrate under nitrification, which is then reduced by denitrifying bacteria to generate nitrogen and discharged under anaerobic conditions, thereby reducing the concentration of total nitrogen in water. [0003] Microbial strains are one of the important biological resources. After the microorganisms with ...

Claims

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Application Information

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IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 孟溪高卫民骆守鹏陶文达崔倩倩陈志刚
Owner HUATIAN ENG & TECH CORP MCC
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