Enhancer for extracting free circulating tumor dna, kit and method for extracting free circulating tumor dna in peripheral blood

Abstract

The invention discloses an enhancer for extracting free circulating tumor DNA, a kit and a method for extracting free circulating tumor DNA in peripheral blood. Wherein, the enhancer includes: 1 μg / μL~5 μg / μL Carrier RNA, 1 μg / μL~5 μg / μL polyadenylic acid, 1 μg / μL~5 μg / μL glycogen, 0~5 μg / μL linear acrylamide, 1 μg / μL ~ 5 μg / μL yeast tRNA, 2 μg / μL ~ 10 μg / μL salmon sperm DNA and / or herring sperm DNA and water. The enhancer used in the extraction of free circulating tumor DNA (ctDNA) of the present invention can be used in combination with conventional lysis binding solutions, washing buffers, eluents, etc., by specifically binding to ctDNA and settling down, significantly improving the ctDNA extraction efficiency and extraction yield.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an enhancer for extracting free circulating tumor DNA, a kit and a method for extracting free circulating tumor DNA in peripheral blood. Background technique [0002] With increasing morbidity and mortality, cancer has become the leading cause of death and a major public health problem in China. Cancer treatment methods are diverse, including radiotherapy, chemotherapy, and emerging targeted therapy and immunotherapy; among them, targeted therapy is an important component of precision medicine, and it is particularly important for the screening of targeted gene-specific populations. Cancer genetic testing It is directly related to the therapeutic efficiency of drugs for patients. At present, the main sample types used for detection are tumor tissue or puncture samples; however, tissue samples are invasive operations, which are sometimes difficult to implement, and these oper...

AI Technical Summary

Problems solved by technology

However, there are still some technical challenges in using ctDNA to detect gene mutations, mainly as follows: 1) ctDNA content varies from person to person, and most people have low content; 2) ctDNA fragments are relatively small, most of them are about 180bp, Fragments are distributed between 100bp and 400bp; 3) The proportion of tumor-associated DNA in ctDNA varies greatly from person to person, and is often difficult to detect due to the small proportion

These limit the wide application of ctDNA in tumor detection. Therefore, efficient and convenient ctDNA extraction is an important factor affecting the wide application of ctDNA in tumor detection.

[0004] At present, commercialized ctDNA extraction kits mainly include column extraction method and magnetic bead method. It is simple and fast, does not require complex instruments, and can be combined with extraction workstations to reduce manual operations. However, the extraction efficiency of commercial magnetic bead extraction kits is average at present, and the problem of extraction efficiency needs to be solved urgently

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