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A miniature photoderivatizer for aflatoxins and sulfonamides

A technology of sulfa drugs and aflatoxin, which is applied in the field of optical detection, can solve the problems of short light source life, high price, time-consuming and laborious, etc., and achieve the effect of reducing production cost, low cost and shortening analysis time

Active Publication Date: 2021-07-27
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the net of such a loop structure is compiled by hand, which is time-consuming and laborious; the price of a commercial photochemical derivatizer is between 30,000 and 40,000 yuan.
In short, the photochemical derivatives in the prior art have many problems such as short light source life, complicated production, large volume, and high price.

Method used

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  • A miniature photoderivatizer for aflatoxins and sulfonamides
  • A miniature photoderivatizer for aflatoxins and sulfonamides

Examples

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Effect test

Embodiment 1

[0034] A post-column light derivatizer for high performance liquid chromatography (HPLC), the excitation light source is a high-power central wavelength 365nm ultraviolet LED, the bandwidth (FWHM) is 10nm, the divergence angle is 60°, and the rated current is 800mA; the derivation reaction tube is highly transparent The FEP tube has an inner diameter of 0.25 mm, an outer diameter of 1 / 16 inch, and a length of 18 cm. The volume of the derivation pool is 10 μL, and the distance between the derivation reaction tube and the LED light source is 7 mm; the derivation reaction tube is spirally wound on the inner surface of the reflective bowl and fixed with a thin steel wire; The LED substrate and heat sink are made of metal aluminum. The photochemical derivatizer was connected with Shimadzu RF-20A fluorescence detector to evaluate its performance. The liquid chromatography conditions are: chromatographic column: C18 column, 4.6mm×150mm×5μm; mobile phase A methanol: mobile phase B pur...

Embodiment 2

[0039] The photochemical derivatizer as described in Example 1 was combined with Shimadzu RF-20A fluorescence detector to analyze and detect 4 kinds of sulfa drugs. RF-20A has an excitation wavelength of 230nm and an emission wavelength of 400nm.

[0040] Experimental results: 5ppb sulfadiazine (SDZ) was injected, and RF-20A was not detected when the photochemical derivatizer was not connected; after the photochemical derivatizer was connected, 5ppb sulfadiazine (SDZ) could be detected, and the detection signal-to-noise ratio was 15.

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Abstract

The invention provides a miniature light derivatizer for aflatoxin and sulfa drugs. It includes a UV LED light source, the excitation wavelength of which is between 280 and 370nm, and uses a thin inner diameter tube transparent to ultraviolet light as the derivation reaction pool. The diameter is 0.10-0.35 mm, the length is 17-30 cm, and the volume of the derivation pool is 7-15 μL. The optical derivatizer of the present invention increases the fluorescence intensity of aflatoxin B1 by 5 times; the derivatizer is used for liquid chromatography post-column derivatization and flow injection analysis derivatization needs, including high performance liquid chromatography (HPLC) and ultra-high pressure liquid Trace aflatoxins and sulfonamides in samples were analyzed by UPLC.

Description

technical field [0001] The patent of the present invention relates to the field of optical detection of high performance liquid chromatography (HPLC) and flow injection analysis (FIA), more specifically, relates to a light derivatizer for aflatoxin and sulfa drugs. Background technique [0002] Photoderivatizers are widely used in the quantitative detection of aflatoxins by high-performance liquid chromatography (HPLC)-fluorescence detection, such as national standards GB / T 18979-2003 and GB / T 23212-2008, and the 2015 Chinese Pharmacopoeia that photoderivatization The instrument is included in the chapter on the determination of aflatoxin, which is used to assist in the determination of trace aflatoxin in medicinal materials, decoction pieces and preparations. Aflatoxin (AFTS) is a molecular mycotoxin, which is known as the strongest natural carcinogen. In 1993, aflatoxin was classified as a class I carcinogen by the Cancer Research Institute of the World Health Organization...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/74G01N21/64
CPCG01N21/643G01N30/02G01N30/74
Inventor 关亚风王楠耿旭辉宁海静冯春波
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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