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Efficient method for enriching functional substances in broccoli bud seedlings

A technology of broccoli sprouts and functional substances, applied in the fields of seed and rhizome treatment, application, agriculture, etc., can solve the problems of unused broccoli, achieve the effect of short enrichment period, improve nutritional value, and simple operation

Active Publication Date: 2019-03-01
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are domestic reports on the effects of environmental stress on plant glucosinolate content, for example, Guo Liping's doctoral thesis "Heat Shock, Hypoxia and JA and ABA Regulate the Formation Mechanism of Sulforaphane in Broccoli Sprouts", Tian Ming's Doctoral thesis "Study on the Effects of Selenium Treatment on the Synthesis and Metabolism of Glucosinolates in Broccoli", Ding Yi's master thesis "Effects of Selenium-Sulphur Interaction on the Growth and Antioxidant Quality of Chinese Cabbage Plants" and thesis "Broccoli Sulfur, Selenium Metabolic Competition and Its Effects on Health Function Research Progress" and others did not conduct heat shock treatment and selenium-sulfur interaction at the same time and did not control the treatment time period, or did not use broccoli as the experimental raw material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Select broccoli seeds with full granules, high germination rate and undamaged as raw materials, soak in 1.5% sodium hypochlorite solution for 15 minutes, then wash with distilled water until neutral pH, and place in 5 times the amount (v / w) of deionized Soak in water at 30°C for 4 h; put the soaked broccoli seeds in a germination machine, germinate at 30°C for 5 days, and spray 10 mL of a mixed aqueous solution of 0.01 mM sodium selenite and 3 mM zinc sulfate every 24 hours during the first 2 days of germination. After 2 days of germination, heat-shock the sprouts at 40°C once a day for 20 minutes each time. During this period, spray the broccoli sprouts with a mixed aqueous solution of 0.1mM sodium selenite and 6mM zinc sulfate for 2 minutes every 24 hours. The spray flow rate is 10ml / min. The content of glucosinolate in broccoli sprouts is about 51 mg / g DW, and the content of selenoamino acid is about 26 μg / g DW.

Embodiment 2

[0021] Select broccoli seeds with full granules, high germination rate and undamaged as raw materials, soak in 1.5% sodium hypochlorite solution for 15 minutes, then wash with distilled water until neutral pH, and place in 6 times the amount (v / w) of deionized Soak in water at 30°C for 4 h; put the soaked broccoli seeds in a germination machine, germinate at 30°C for 5 days, and spray 15 mL of a mixed aqueous solution of 0.05 mM sodium selenite and 2 mM zinc sulfate every 24 hours during the first 2 days of germination. After 2 days of germination, heat-shock the sprouts at 40°C once a day for 15 minutes each time. During this period, spray the broccoli sprouts with a mixed aqueous solution of 0.1mM sodium selenite and 10mM zinc sulfate for 2 minutes every 16 hours. The spray flow rate is 6ml / min. The content of glucosinolate in broccoli sprouts is about 56 mg / g DW, and the content of selenoamino acid is about 24 μg / g DW.

Embodiment 3

[0023] Select broccoli seeds with full granules, high germination rate and undamaged as raw materials, soak in 1.5% sodium hypochlorite solution for 15 minutes, then wash with distilled water until neutral pH, and place in 6 times the amount (v / w) of deionized Soak in water at 30°C for 4 h; put the soaked broccoli seeds in a germination machine, germinate at 30°C for 7 days, and spray 5 mL of a mixed aqueous solution of 0.2 mM sodium selenite and 1 mM zinc sulfate every 24 hours during the first 2 days of germination. After 2 days of germination, place the sprouts at 37°C for heat shock once a day for 20 minutes each time. During this period, spray the broccoli sprouts with a mixed aqueous solution of 1mM sodium selenite and 6mM zinc sulfate for 2min every 24h. The shower flow rate is 10ml / min. The content of glucosinolate in broccoli sprouts is about 60mg / g DW, and the content of selenoamino acid is about 33μg / g DW.

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Abstract

The invention relates to an efficient method for enriching functional substances in broccoli bud seedlings, and belongs to the technical field of food biotechnology. The method comprises the specificsteps that within 5-7 days after germination of broccoli seeds, the seeds normally germinate in first two days without stress, after two days of seed germination, in the bud seedling growing process,the seedlings are subjected to high-temperature stress once every day at 35-60 DEG C for 15-25 min, 0.1-1 mM sodium selenite and a 6-10 mM zinc sulfate mixed aqueous solution are used for conducting spraying stress for 1-2 min every 16-24 h, and the flow of sprayed liquid is 5-10 mL / min. The raw material application range is wide, the operation is simple, and the production cost is low. After thebroccoli bud seedlings are subjected to heat shocking and selenium-sulfur interaction stress, the content of glucosinolate and seleno-amino acid in the generated broccoli bud seedlings is five-ten times that of the broccoli bud seedlings before treatment, and efficient enrichment of the functional substances such as the glucosinolate, the seleno-amino acid and the like can be achieved.

Description

technical field [0001] The invention relates to a high-efficiency enrichment method for functional substances in broccoli sprouts, belonging to the field of food biotechnology. Background technique [0002] Glucosinolates (referred to as glucosinolates) are a class of secondary metabolites containing nitrogen and sulfur. Glucosinolates (GRA) and its metabolites sulforaphane (ICTs) have been confirmed to be associated with the flavor and nutritional components of cruciferous vegetables. , plant self-protection mechanisms and human health are closely related. Broccoli belongs to the biennial Brassica plant, and a large number of studies have confirmed that it can effectively enrich organic selenium. Compared with other plant species, Brassica plants not only have the ability to efficiently synthesize selenoamino acids, selenoproteins, and selenoglucosides, but also have the most abundant glucosinolates among more than 450 species of organisms that contain glucosinolates in na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 尹永祺韩宇朱振宇沈敏熙程雨薇方维明
Owner YANGZHOU UNIV
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