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A kind of bladder repair material and its use and preparation method

A technology for repairing materials and antibodies, applied in the fields of medical tissue engineering and bladder tissue repair, can solve problems such as difficulty in effectively promoting muscle regeneration and few smooth muscle cells, and achieve excellent repair performance, good recovery effect, and the effect of promoting regeneration.

Active Publication Date: 2019-12-20
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Urine-derived stem cells are a new type of stem cells discovered in recent years, but there are still problems in using them as seed cells to effectively promote muscle regeneration, such as the research by Liu Yang et al. (Stem Cell Research & Therapy, 2017, 8(1 ):63), taking rabbits as the experimental object, four weeks after the operation, the new smooth muscle cells were still very few

Method used

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  • A kind of bladder repair material and its use and preparation method
  • A kind of bladder repair material and its use and preparation method
  • A kind of bladder repair material and its use and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 Preparation of a new tissue repair material

[0049] 1. Preparation of SIS

[0050] (1) Cleaning the intestines: take a pair of fresh pig small intestines, remove excess chyme in the intestines, turn the small intestines inside out, and clean the inner surface repeatedly with normal saline, then cut the small intestines longitudinally with scissors, and finally cut them into long lengths Intestine segment about 15cm.

[0051] (2) Physical curettage: use a spatula to scrape off the serosa layer and muscle layer on the intestinal segment to obtain a preliminary SIS. This process needs to be repeatedly scraped off until the milky white translucent film is separated, and washed 2-3 times with normal saline.

[0052] (3) Degreasing: After repeatedly rinsing with deionized water, wrap the SIS membrane with gauze, squeeze out part of the water, and remove excess water with filter paper. After mixing the chloroform-methanol mixture at 1:1 v / v, soak the dried SIS...

Embodiment 2

[0065] Embodiment 2 Preparation of a tissue repair material

[0066] 1. Preparation of SIS

[0067] With embodiment 1 step 1.

[0068] 2. Thiolation of SIS Membranes

[0069] (1) Cut the SIS material into discs with d = 15.6 mm (24-well plate size), and place 10 SIS discs in a glass dish with d = 60 mm;

[0070] (2) Prepare 2ml of Traut's solution of 5mg / ml with PBS;

[0071] (3) Completely soak the materials (10 tablets / dish) in the step (1) in Traut's solution, and react at room temperature for 1.5 hours. After the reaction, wash with PBS 2-3 times.

[0072] 3. Coupling of Antibody to SIS Membrane

[0073] This example takes the antibody CD29 as an example to illustrate the coupling process of the antibody to the SIS membrane. 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid sulfosuccinimidyl ester sodium salt (Sulfo-SMCC; sigma) was prepared at a concentration of 0.5 mg / ml in 100 μl of PBS React with 10 μl (1 mg / ml) CD29 antibody in a glass dish at room temperature...

Embodiment 3

[0076] Embodiment 3 Preparation of a tissue repair material

[0077] 1. Preparation of SIS

[0078] With embodiment 1 step 1.

[0079] 2. Thiolation of SIS Membranes

[0080] (1) Cut the SIS material into discs with d = 15.6 mm (24-well plate size), and place 10 SIS discs in a glass dish with d = 60 mm;

[0081] (2) Prepare 2ml of Traut's solution of 10mg / ml with PBS;

[0082] (3) Completely soak the materials (10 tablets / dish) in the step (1) in Traut's solution, and react at room temperature for 1.5 hours. After the reaction, wash with PBS 2-3 times.

[0083] 3. Coupling of Antibody to SIS Membrane

[0084] This example takes the antibody CD29 as an example to illustrate the coupling process of the antibody to the SIS membrane. With 100 μl, the 0.5mg / ml 4-(N-maleimidomethyl)cyclohexane-1-carboxylic acid sulfosuccinimide sodium salt (Sulfo-SMCC; sigma) prepared in PBS was mixed with 10 μl (1 mg / ml) of CD29 antibody was reacted in a glass dish for 1.5 hours at room temp...

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Abstract

The invention discloses a bladder repair material, and further discloses a preparation method and application of the bladder repair material. The bladder repair material is a small intestinal mucosa lower-layer film composited with an antibody, wherein the antibody is one or more of CD29, CD44, CD73 and CD90. The repair material has the capacity of collecting urine-derived stem cells in urine andcan be used for bladder repair; and the bladder repair material has the very low cell pollution risk, good biocompatibility and excellent bladder repair capacity.

Description

technical field [0001] The invention relates to the field of medical tissue engineering, especially the field of bladder tissue repair. [0002] technical background [0003] Bladder injury refers to the contusion, laceration and contusion of the bladder tissue structure caused by various violence; cystectomy is to remove the bladder lesion tissue when necessary (such as bladder cancer, etc.). At present, gastrointestinal replacement is often used to repair bladder tissue clinically, but there are many complications, such as mucus secretion, long-term bacteriuria, stone formation, anastomotic leakage, electrolyte disturbance, etc., and may cause new damage. [0004] The emergence of tissue engineering technology provides a new way to solve this problem. At present, there are many reports on the method of tissue engineering scaffold combined with seed cells for bladder repair. This method has advantages in promoting bladder tissue regeneration and preventing scar formation. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/54
CPCA61L27/3629A61L27/3679A61L27/3687A61L27/50A61L27/54A61L2300/256A61L2300/412A61L2430/22
Inventor 解慧琪李艳清江燕林
Owner WEST CHINA HOSPITAL SICHUAN UNIV