Suture-resistant collagen-based corneal regeneration and repair material and preparation method thereof

A technology for collagen-based cornea and repair materials, applied in suture-resistant collagen-based cornea regeneration and repair materials and its preparation, can solve the problems of insufficient tensile strength and unfavorable conventional suture, and achieve low cost, good composite stability, and biological good compatibility effect

Active Publication Date: 2019-11-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, each of these existing implants has drawbacks, including insufficient tensile strength, which is not conducive to conventional suturing, etc.

Method used

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  • Suture-resistant collagen-based corneal regeneration and repair material and preparation method thereof
  • Suture-resistant collagen-based corneal regeneration and repair material and preparation method thereof
  • Suture-resistant collagen-based corneal regeneration and repair material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Shred the purified bovine Achilles tendon type Ⅰ collagen with scissors, dissolve it with 0.01mol / L hydrochloric acid solution, and use a laboratory high-shear mixer to shear at high speed under ice bath conditions, and the speed is 6000-7000rpm ;

[0029] (2) Continue stirring the Col solution obtained in step (1) with a stirring paddle, and slowly add a certain amount of 15 mg / mL EDC / NHS cross-linking agent into the stirring Col solution, and stir at 4°C for 24 hours , wherein the mass ratio of Col:EDC:NHS is 6:1:1;

[0030] (3) Get a small amount of crosslinked Col solution in step (2) and concentrate in a far-infrared drying oven, and calculate the concentration of Col solution, and adjust the final concentration of Col solution to be 5mg / mL;

[0031] (4) Slowly stir the Col solution obtained in step (3) at room temperature for 2 hours, and centrifuge to remove air bubbles, wherein the rotating speed of the stirring paddle is 120rpm;

[0032] (5) Inject the Co...

Embodiment 2

[0037] (1) Cut the purified bovine Achilles tendon type Ⅰ collagen with scissors, dissolve it with 0.01mol / L hydrochloric acid solution and shear it at high speed with a laboratory high-shear mixer under ice bath conditions, and the speed is 6000-7000rpm ;

[0038] (2) Continue to stir the Col solution obtained in step (1) with a stirring paddle, and slowly add a certain amount of 15mg / mL EDC / NHS crosslinking agent into the stirring Col solution, and stir at 4°C for 24h , wherein the mass ratio of Col:EDC:NHS is 6:1:1;

[0039] (3) Get a small amount of crosslinked Col solution in step (2) and concentrate in a far-infrared drying oven, and calculate the concentration of Col solution, and adjust the final concentration of Col solution to be 6mg / mL;

[0040] (4) Slowly stir the Col solution obtained in step (3) at room temperature for 2 hours, and centrifuge to remove air bubbles, wherein the rotating speed of the stirring paddle is 120rpm;

[0041](5) Inject the Col solution ...

Embodiment 3

[0045] (1) Cut the purified bovine Achilles tendon type Ⅰ collagen with scissors, dissolve it with 0.01mol / L hydrochloric acid solution and shear it at high speed with a laboratory high-shear mixer under ice bath conditions, and the speed is 6000-7000rpm ;

[0046] (2) Continue to stir the Col solution obtained in step (1) with a stirring paddle, and slowly add a certain amount of 15mg / mL EDC / NHS crosslinking agent into the stirring Col solution, and stir at 4°C for 24h , wherein the mass ratio of Col:EDC:NHS is 6:1:1;

[0047] (3) Get a small amount of crosslinked Col solution in step (2) and concentrate in a far-infrared drying oven, and calculate the concentration of Col solution, and adjust the final concentration of Col solution to be 6.5mg / mL;

[0048] (4) Slowly stir the Col solution obtained in step (3) at room temperature for 2 hours, and centrifuge to remove air bubbles, wherein the rotating speed of the stirring paddle is 120rpm;

[0049] (5) Inject the Col soluti...

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Abstract

The invention belongs to the field of biomedical materials, and discloses a suture-resistant collagen-based corneal regeneration and repair material and a preparation method thereof. The preparation method comprises the following steps: adding collagen to hydrochloric acid to dissolve collagen, then adding an EDC / NHS cross-linking agent for a cross-linking reaction, heating and concentrating to obtain a cross-linked collagen solution; defoaming, adding the defoamed product to a petri dish to reach a certain thickness, then tiling a PCL circular ring to the bottom of the cross-linked collagen solution, air-drying, and washing to obtain a composite film; and hot-pressing the composite film in a die at 100-120 DEG C for secondary molding to obtain the suture-resistant collagen-based corneal regeneration and repair material. The suture-resistant collagen-based corneal regeneration and repair material obtained by the invention has good biocompatibility and is resistant to conventional suture during surgery. In addition, corneal epithelial cells can adhere to and proliferate on the surface of the material. Thereby, rapid epithelization of the corneal epithelial cells on the graft duringsurgery is realized, and finally the purpose of corneal defect repair and corneal replacement is achieved.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a suture-resistant collagen-based corneal regeneration repair material and a preparation method thereof. Background technique [0002] The integrity of the cornea, the transparent, avascular tissue on the surface of the human eye, is important for normal vision. Many factors, such as mechanical, thermal, chemical damage and microbial infection, can make the transparent cornea appear grayish-white turbidity, which can cause vision loss or even blindness, which is called corneal disease. Corneal disease has a high prevalence rate and strong blindness. At present, in the clinical treatment of corneal injury, antibiotics, steroid hormones, collagenase inhibitors, anti-oxidation and other drug treatments, amniotic membrane transplantation, autologous corneal limbal stem cell transplantation and corneal transplantation and other surgical treatments can achieve mild and moderate cor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/18A61L27/50
CPCA61L27/18A61L27/24A61L27/50A61L2430/16C08L67/04
Inventor 任力孙晓敏杨向璟贾永光
Owner SOUTH CHINA UNIV OF TECH
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