Application of ftz-f1 gene, encoded protein and dsrna of gypsy moth in pest control
A FTZ-F1, pest control technology, applied in the field of molecular biology, can solve problems such as gypsy moth drug resistance and pollution of the environment
Active Publication Date: 2021-09-28
HEZE UNIV
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Problems solved by technology
[0004] The present invention aims to solve the problems of drug resistance and environmental pollution in the existing chemical control of gypsy moth, and provides an application of gypsy moth FTZ-F1 gene, its encoded protein and its dsRNA in pest control
Method used
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specific Embodiment approach 1
[0037] Embodiment 1: The nucleotide sequence of the gypsy moth FTZ-F1 gene in this embodiment is shown in SEQ ID NO: 1 in the sequence listing. The nucleotide sequence of its coding region CDS is shown in SEQ ID NO: 2 in the sequence listing.
specific Embodiment approach 2
[0038] Specific embodiment 2: The amino acid sequence of the protein encoded by the coding region CDS of the gypsy moth FTZ-F1 gene in this embodiment is shown in SEQ ID NO: 3 in the sequence listing.
specific Embodiment approach 3
[0039] Specific embodiment three: The dsRNA sequence of the gypsy moth FTZ-F1 gene in this embodiment is shown in SEQ ID NO: 4 in the sequence listing.
[0040]In this embodiment, the gypsy moth FTZ-F1 gene fragment is used as a template, and a specific dsRNA primer pair is designed, and the FTZ-F1 gene dsRNA is synthesized by a MEGAscript RNAi kit (Ambion).
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Abstract
The application of the gypsy moth FTZ-F1 gene, its encoded protein and its dsRNA in pest control relates to the field of molecular biology, in particular to the gypsy gypsy moth FTZ-F1 gene, its encoded protein and its application. The invention aims to solve the problems of drug resistance and environmental pollution in the existing chemical control of the gypsy moth. The nucleotide sequence of the gene is shown as SEQ ID NO: 1 in the sequence listing. The amino acid sequence of the protein encoded by the CDS of its coding region is shown in SEQ ID NO: 3 in the sequence listing. The invention utilizes molecular biological means to interfere with the gene FTZ-F1 to down-regulate its expression, prolong the molting cycle of larvae, slow down its development, and even cause death. The 84K poplar plant transformed with dsRNA capable of expressing the gypsy moth FTZ-F1 gene provided by the present invention can significantly hinder the stretching of the adult wing of the gypsy moth, and affect the development and flight ability of the adult wing. The invention is used in the field of gypsy moth pest control.
Description
technical field [0001] The invention relates to the field of molecular biology, in particular to the gypsy moth FTZ-F1 gene, its encoded protein and its application. Background technique [0002] Gypsy moth is an important agricultural and forestry leaf-eating pest. It is widely distributed in my country and can harm more than 500 species of plants such as poplar, willow, apple, sylvestris pine, and larch. It can cause huge economic losses in large outbreaks. At present, chemical control still occupies the main position in the control of gypsy moth pests, and biological control and physical control are usually only used as auxiliary control methods. The long-term, high-frequency, and continuous increase in the use of chemical pesticides has led to insect resistance, rampant growth, and environmental pollution such as pesticide residues. The emergence of disadvantages after the use of chemical pesticides has promoted the development of a new generation of pest control resear...
Claims
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IPC IPC(8): C12N15/12C12N15/82A01H5/00A01H6/00C07K14/435
CPCC07K14/43563C12N15/8286
Inventor 王步勇问荣荣王娟李莹莹李秀伟
Owner HEZE UNIV