A kind of entomopathogenic nematode preparation and its preparation method and application

A technique for entomopathogenic nematodes and preparations, which is applied in the field of entomopathogenic nematode preparations and preparations, can solve the problems of short persistence period and poor EPN field use effect, and achieve short persistence period, poor solution effect and improved quick effect sexual effect

Active Publication Date: 2020-09-22
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem solved by the present invention is: to provide an entomopathogenic nematode preparation, which solves the problems of poor field use effect and short duration of EPN in the prior art

Method used

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  • A kind of entomopathogenic nematode preparation and its preparation method and application
  • A kind of entomopathogenic nematode preparation and its preparation method and application
  • A kind of entomopathogenic nematode preparation and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] This example provides the ingredient content table and preparation method of the entomopathogenic nematode preparation of the present invention, see Table 1 for details.

[0044] Table 1

[0045]

[0046] Adopt the raw material in table 1 to prepare the method for making entomopathogenic nematode preparation of the present invention as follows:

[0047] Step 1. Propagation and cultivation of entomopathogenic nematodes:

[0048] For entomopathogenic nematodes (Heterorhabditis beicherriana), the last instar larvae of Galleria mellonella (about 0.20g / piece in weight) or mature larvae of Tenebrio molitor (about 0.10g / piece in weight) are used for propagation; The third-instar larvae (IJs) of the entomopathogenic nematode infection stage were collected into distilled water by the White trap method, and stored in a refrigerator at 4°C for about 2 weeks before use;

[0049] Step 2. Take each raw material component in proportion:

[0050] Concentrate the entomopathogenic ...

Embodiment 2

[0052] This example provides a composition content table of the entomopathogenic nematode preparation of the present invention, see Table 2 for details.

[0053] Table 2

[0054]

[0055]

[0056] Adopt the raw material in table 2 to prepare the method for making entomopathogenic nematode preparation of the present invention as follows:

[0057] Step 1. Propagation and cultivation of entomopathogenic nematodes:

[0058] For entomopathogenic nematodes (Heterorhabditis beicherriana), the last instar larvae of Galleria mellonella (about 0.20g / piece in weight) or mature larvae of Tenebrio molitor (about 0.10g / piece in weight) are used for propagation; The third-instar larvae (IJs) of the entomopathogenic nematode infection stage were collected into distilled water by the White trap method, and stored in a refrigerator at 4°C for about 2 weeks before use;

[0059] Step 2. Take each raw material component in proportion:

[0060] Concentrate the entomopathogenic nematode (He...

Embodiment 3

[0062] This example provides the application of the preparation of the present invention to prevent and control peanut grubs.

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PUM

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Abstract

The invention discloses an EPN (entomopathogenic nematode) preparation and a preparation method and application thereof, which can solve the problems of poor use effect and short lasting period in thefield use process of EPN in the prior art. The EPN preparation comprises heterorhabditis beicherriana and thiourea. The invention also discloses the application of the EPN preparation in preparing ofpesticides for preventing and controlling underground pests of plants. The EPN preparation has the advantages that the fertilizer synergist compound (thiourea) in the fertilizer of the traditional agriculture and the biocontrol preparation (EPN) are combined to use, so as to improve the quick-effect property of the EPN in the actual agriculture production, and further solve the problems of poor use effect and short lasting period in the field use process of EPN.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to an entomopathogenic nematode preparation, a preparation method and an application. Background technique [0002] Pest control has always been one of the ways for people to increase crop yields. Chemical pesticides have once become an effective tool for pest control due to their high-efficiency and rapid insecticidal effects. However, with the extensive use of chemical pesticides, a series of potential dangers and Disadvantages have also gradually emerged. Environmental pollution, the development of pest resistance and the re-emergence of pests have become the main factors restricting the development and application of chemical pesticides. In order to meet the continuous improvement of human requirements for the environment and food safety, biological control, as a safe and effective means, has received extensive attention. Entomopathogenic Nematode (EPN...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/12A01N63/14A01N47/28A01P7/04
CPCA01N47/28A01N63/10
Inventor 李星月张鸿刘奇志杨武云李其勇陈诚朱从桦符慧娟
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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