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Penicillium oxalicum strain for inhibiting banana wilt and application of penicillium oxalicum strain

A technology for banana fusarium wilt and Penicillium oxalicum, applied in the field of microorganisms, can solve the problems of differences in the effects of fusarium wilt and differences in the diversity of antagonistic microorganisms

Active Publication Date: 2019-03-19
HUAQIAO UNIVERSITY
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Problems solved by technology

However, due to the sensitivity of soil microorganisms to soil physical and chemical properties in the environment, soil pH value, and local climate, the diversity of antagonistic microorganisms in the soil of different banana production areas is significantly different, and the effect of the same strain on controlling Fusarium wilt in large-scale cross-regional banana orchards is different. significantly

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  • Penicillium oxalicum strain for inhibiting banana wilt and application of penicillium oxalicum strain
  • Penicillium oxalicum strain for inhibiting banana wilt and application of penicillium oxalicum strain
  • Penicillium oxalicum strain for inhibiting banana wilt and application of penicillium oxalicum strain

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Embodiment Construction

[0029] Below by embodiment the content of the present invention is specified:

[0030] 1. Isolation and screening of antagonistic strains

[0031] (1) Collection of soil samples and strains of banana fusarium wilt

[0032] Soil was obtained from banana orchards in Zhangzhou, Fujian.

[0033] The pathogenic bacteria of Fusarium wilt of banana is race number FOC4, provided by Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences.

[0034] (2) Isolation of antagonistic bacteria

[0035] The collected soil samples were naturally dried indoors, crushed, and passed through a 100-mesh sieve. Weigh 1 g of the sieved sample, add 100 mL of sterilized water, shake vigorously for 30 min, and dilute to 10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 g / mL concentration gradient, mix well. Take 1mL of the diluted solution and add it to the selective medium cooled to about 50°C, shake it slightly, and culture it at 28°C for 3-5 days, pick different types of colonies, ...

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Abstract

The invention discloses a penicillium oxalicum strain for inhibiting banana wilt and an application of the penicillium oxalicum strain. A microbial strain is separated and screened from soil, is identified as Penicillium oxalicum in combination with morphological features and molecular identification and is preserved with the serial number: CCTCC NO:M2018183 in China Center for Type Culture Collection located in Wuhan University, Wuhan, China. The strain is capable of obviously inhibiting the incidence rate of banana FOC and improving the growth vigor of banana seedlings and good in wilt preventing and controlling effect and application potential.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a Penicillium oxalic acid strain for inhibiting banana wilt and application thereof. Background technique [0002] my country is an important banana producer in the world. In 2015, the national planting area reached 431,100 hectares, and the output reached 12.6416 million tons. However, the sound development of my country's banana industry is suffering from the severe test of Fusarium wilt (Fusuraum oxysporum f.sp.cubense, FOC). FOC is a soil fungal disease that severely damages the vascular tissue of bananas, leading to the death of infected banana seedlings in a short period of time, which greatly reduces the income of ordinary people and discourages the enthusiasm of fruit farmers for production. [0003] Chemical pesticide control is still the main technical means for the control of banana Fusarium wilt in my country. However, the use of chemical pesticides not onl...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00A01G13/00C12R1/80
CPCA01G13/00A01N63/30C12N1/14C12N1/145C12R2001/80
Inventor 王明元徐志周刘建福张敏瑜侯式贞于海玲
Owner HUAQIAO UNIVERSITY
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