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A kind of inactivation method of Avian bacilli paragallinarum

A technology for inactivating chickens and poultry, which is applied in the directions of microorganism-based methods, methods of using microorganisms, biochemical equipment and methods, etc., which can solve the problem of long inactivation cycle of Avian bacillus paragallinarum bacteria liquid, and residual vaccine safety inactivator. and other problems, to achieve the effect of overcoming inactivator residues, improving safety, and improving immune activity.

Active Publication Date: 2021-10-29
TIANJIN RINGPU BIO TECH
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is also to solve the problem of vaccine safety and inactivating agent residues in animals after adding the inactivating agent
[0006] The purpose of the present invention is to provide a novel bacterial liquid inactivation process, to solve the technical problem of the long inactivation cycle of Avian bacillus paragallinarum bacterial liquid in the prior art

Method used

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  • A kind of inactivation method of Avian bacilli paragallinarum
  • A kind of inactivation method of Avian bacilli paragallinarum

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Embodiment 1

[0022] Embodiment 1: the research of bacteria liquid inactivation process

[0023] Bacterial species: Avibacterium paragallinarum type A C-Hpg-8 strain, provided by Tianjin Ruipu Biotechnology Co., Ltd. Airport Economic Zone Company.

[0024] Culture medium: semi-synthetic medium, chicken broth medium, chicken broth agar, the formula refers to the "Manufacturing and Testing Regulations of Inactivated Chicken Infectious Rhinitis Vaccine", which is self-made by our laboratory.

[0025] Bacteria solution for inactivation: According to the regulations, after the cultured bacteria solution is centrifuged with a centrifuge, and then made into a suspension with phosphate buffer, it is estimated that each ml of concentrated solution contains 10 billion bacteria. The above bacterial liquid is 100ml.

[0026] 1. Divide the above bacterial solution into 3 parts and place them in water baths at 45°C, 50°C, 60°C, and 65°C for inactivation, and shake and mix well during the period. 200 μL...

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Abstract

The invention provides a method for inactivating Avian bacillus paragallinarum bacteria liquid, which belongs to the field of bacteria liquid inactivation technology. The inactivation method is 50-65°C, stir for 30-45 minutes, then put it in 2-8°C, store it in cold storage for 3 days, and then it will be completely inactivated. The invention has simple inactivation process, short time and thorough inactivation, can effectively avoid adverse effects on antigen immunogenicity and vaccine safety due to the addition of an inactivator, and can significantly improve the vaccine production process and quality improvement.

Description

technical field [0001] The invention belongs to the field of production technology of veterinary biological products, especially the field of bacterial liquid inactivation technology. Background technique [0002] Avian infectious rhinitis is an acute upper respiratory infectious disease of chickens caused by Avibacterium paragallinarum. When the disease occurs alone, it can cause the egg production of laying hens to decrease, the growth and development of bred chickens will be hindered and the elimination rate will increase, and the mortality rate is generally not high. When the disease is complicated, the symptoms will be aggravated, the course of the disease will be prolonged, and the mortality rate will increase. When the disease occurs in broilers, it will cause a serious decline in meat quality, increase the death rate, and cause great economic losses. [0003] At present, the prevention and treatment of chicken infectious rhinitis mainly adopts two methods of drug or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36C12N1/20C12R1/01
CPCC12N1/20C12N1/36
Inventor 李守军陈冰郁宏伟康亚男张立霞唐荣宏丁旭娜
Owner TIANJIN RINGPU BIO TECH
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