Kit for detecting human immune ages and application of kit

A technology for detecting human body and kit, applied in the field of medicine, can solve the problem of lack of a mature detection method, and achieve the effect of good repeatability, high sensitivity and strong specificity

Inactive Publication Date: 2019-03-19
ZHEJIANG BOZHEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, no medical testing institution has proposed the detection of human immune

Method used

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  • Kit for detecting human immune ages and application of kit
  • Kit for detecting human immune ages and application of kit

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0068] The preparation method of the kit comprises the following steps:

[0069] 1) Fluorescently label the antibody;

[0070] 2) Metering the fluorescently labeled antibody according to the formula amount;

[0071] 3) Add the fluorescently labeled antibody measured above into a light-proof plastic or glass reagent bottle, mix evenly, and screw the cap to seal to obtain a kit.

[0072] The storage conditions of the kit are: 2-8°C, protected from light.

[0073] After the preparation of the kit is completed, the human peripheral blood from the above 800 samples is tested; the human body includes physical examination persons of all ages, and is required to be free of thymoma or thymus-related diseases, and is not used to stimulate the immune system. drug.

[0074] When the kit is used for detection, the preparation of the detection sample includes the following steps:

[0075] 1) Collect not less than 100 μL of human peripheral blood as a test sample; the test sample is test...

Embodiment 1

[0089] Preparation of reagents:

[0090] CD3-FITC, CD4-PerCP, CD31-PE, CD45RA-APC four kinds of monoclonal antibodies, take 125 μ L of each antibody respectively, add in light-proof plastic or glass reagent bottle, mix evenly, namely become 100 servings of the present invention product, corresponding to the dosage of the product per person is 5 μL.

Embodiment 2

[0092] Preparation of reagents:

[0093] CD3-FITC, CD4-PerCP, CD31-PE, CD45RA-APC four kinds of monoclonal antibodies, take 50 μL of each antibody respectively, add in light-proof plastic or glass reagent bottle, mix evenly, namely become 100 servings of the present invention product, corresponding to the dosage of the product per person is 2 μL.

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Abstract

The invention provides a kit for detecting human immune ages and an application of the kit. Reagents in the kit comprise CD3, CD4, CD31 and CD45RA monoclonal antibodies which are four fluoresceins applicable to flow cytometers, and the colors of the four fluoresceins are different. The reagents in the kit comprise, in weight percent, 0.66-94.34% of fluorescent labeled CD3, 0.66-94.34% of fluorescent labeled CD4, 0.66-94.34% of fluorescent labeled CD31 and 0.66-94.34% of fluorescent labeled CD345RA. The kit is used for detecting recently outputted cell number of human thymuses, the immune agesof people are determined according to detection results of the recently outputted cell number of the human thymuses, and the immune ability of a human body is evaluated.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a kit and application thereof. The kit is used for detecting human immune age. Background technique [0002] The thymus is an important lymphatic organ of the body, not only the central immune organ of the human immune system, but also a gland of the endocrine system. As the central control organ of the immune system, its function is closely related to immunity, and plays an important role in the establishment, improvement and function of the entire human immune system. [0003] With the increase of age, the thymus undergoes obvious pathological changes, most of which are replaced by adipose tissue, leaving very little thymic cortex or medulla. Thus, it has long been assumed that an age-related process of thymic evolution is accompanied by non-functioning thymus. [0004] However, recent studies have shown that thymic hyperplasia, despite an inverse relationship with age, can ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/58G01N33/533G01N15/14
CPCG01N33/577G01N15/14G01N33/533G01N33/582
Inventor 倪万茂迟妍妍陈乐芝倪万根倪声雷张鸿张鸿君林鹏程刘维杰朱阳承
Owner ZHEJIANG BOZHEN BIOTECH CO LTD
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