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Strain of bletilla striata endophytic fungus 1-G1 and application thereof

An endophytic fungus, 1-G1 technology, applied in applications, fungi, biocides, etc., can solve the problems of lack of quality standards for seedlings, restricting the sustainable development of the industry, and lagging in the research and development and application of varieties and quality control technologies. The effect of strong growth promoting effect and broad application prospect

Active Publication Date: 2019-03-22
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the lack of quality standards for seedlings and the lagging behind in the development and application of varieties and quality control technologies are all key issues restricting the sustainable development of the white and white industry.

Method used

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  • Strain of bletilla striata endophytic fungus 1-G1 and application thereof
  • Strain of bletilla striata endophytic fungus 1-G1 and application thereof
  • Strain of bletilla striata endophytic fungus 1-G1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 : Isolation, screening and identification of bacterial strains

[0021] 1.1 Isolation of strains

[0022] Materials for testing: Wild Baiji collected from Ziyuan County and Huanjiang Autonomous County in Guangxi.

[0023] Medium: 1000ml potato dextrose agar medium (PDA medium); PDA medium contains 300g of potatoes, 20g of glucose, 20g of agar, the pH of the medium is 6.0±0.2; DE medium contains 0.111mg of CaCl 2 , 0.174 mg K 2 SO 4 , 27.8mg FeSO 4 ·7H 2 0, 6.501 mg MnCl 2 4H 2 0,0.241mg NaMo 4 , 0.123mg MgSO 4 ·7H 2 0,0.0544 mg KH 2 PO 4 , 1.545 mg H 3 BO 4 , 0.80516mg ZnSO 4 ·7H 2 0,52.108 mg Na 2 EDTA·2H 2 0.9g soluble starch, 1g yeast extract, 8g agar, 1000mL water, pH 6.0.

[0024] Surface disinfection: Gently shake off the soil and attachments on the roots and tubers of the wild white root and tuber, rinse under running water, put the washed white root and tuber on filter paper to absorb the water and set aside. On the ultra-clean wor...

Embodiment 2

[0061] Example 2 : Detection of the growth-promoting characteristics of active strains

[0062] 2.1 Detection of indoleacetic acid (IAA) synthesis ability of active strain 1-G1 fermentation broth

[0063] Accurately weigh 1 mg of IAA, add 10 mL of distilled water to dissolve, and obtain a 100 μg / mL IAA reference solution. Gradual dilution of the IAA reference solution was 10, 20, 30, 40, 50, 60, 70, 80ug / mL. The IAA solution of each gradient and the Salkowski colorimetric solution were mixed at a volume ratio of 1:1, and after being placed at room temperature in the dark for 30 min, the OD530 of the IAA solution of each concentration was measured. With the concentration of IAA as the abscissa and the OD530 of IAA solutions of various concentrations as the ordinate, draw a standard curve.

[0064] Add 20mL IAA detection medium into a 50mL sterilizable centrifuge tube, sterilize at 121°C for 20min, take it out and cool it for later use. On the ultra-clean workbench, use a p...

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PUM

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Abstract

The invention belongs to the technical field of microbial application, in particular to a strain of bletilla striata endophytic fungus 1-G1 and application thereof. The strain of bletilla striata endophytic fungus 1-G1 is classified and named as Diaporthe spectabilis. The strain has been deposited in Guangdong Cultures Collection Center on June 06, 2018 with the preservation number of GDMCCNO.60382. For the first time, the strain of endophytic fungus (Diaporthe spectabilis) 1-G1 is isolated and screened from the medicinal plant bletilla striata, and the strain has a relatively strong promotingeffect on the growth of bletilla striata, and brings a broad application prospect for the yield and quality control of bletilla striata.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a white and endophytic fungus 1-G1 and its application. Background technique [0002] The prevention and control of plant diseases in modern agriculture is overly dependent on the use of chemical pesticides. The application of a large number of chemical pesticides not only has long-term damage to the ecological environment, but also causes the quality of agricultural products to decline, pesticide residues exceed the standard, pathogenic bacteria are resistant to drugs, and are harmful to humans and animals. Many questions. It is of great significance to find safer and more effective methods of disease control, and the use of biological methods to prevent and control plant diseases can effectively solve the above problems. [0003] Baiji Bletilla striata (Thunb.) Reichb.F. is a perennial herb belonging to Orchidaceae Bletilla striata, named Baijier,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 李良波田佩雯黄荣韶
Owner GUANGXI UNIV
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