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Compound spore powder prepared through synergistic solid fermentation of mixed strains of trichoderma and application thereof

A technology of solid fermentation and mixed bacteria synergy, which is applied in the fields of application, fertilizer mixture, and the method of using spores. It can solve the problems of single product function, many fermentation batches, and high cost, and achieve increased functions, increased growth, and simple processes. Effect

Inactive Publication Date: 2019-03-26
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One object of the present invention is to provide the composite spore powder prepared by Trichoderma mixed bacteria synergistic solid fermentation, the composite spore powder prepared by Trichoderma mixed bacteria coordinated solid fermentation is used to solve the problem of Trichoderma biocontrol products obtained by fermentation of a single strain or After a single strain is fermented, it is mixed and compounded, which leads to the problems of single product function, many fermentation batches, and high cost. Another purpose of the present invention is to provide the application of the composite spore powder prepared by this Trichoderma mixed bacteria collaborative solid fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step 1. Strain activation and spore suspension preparation

[0025] Three fermentation strains: Trichoderma viride, Trichoderma harzianum and Trichoderma aculeatus were activated on PDA medium, cultured at 28°C for 72 h, and the conidia were washed with sterilized distilled water to make their respective spore suspensions , the number of spores in the spore suspension is 10 7 A / mL.

[0026] Step 2, preparation of solid seeds

[0027] Mix bran, cassava bagasse, bagasse, and corn flour into a seed fermentation material at a mass ratio of 6.8:1.2:1:1, then mix the seed fermentation material and water at a ratio of 1:1 (mass ratio), and pack them separately Put three 500mL plastic bottles, the filling volume is 1 / 5 of the plastic bottle height, and then sterilize at 121℃ for 20 minutes. In the process, the spore suspension of Trichoderma harzianum was added to the second plastic bottle according to the inoculum amount of 1%, the spore suspension of Trichoderma aculeatus ...

Embodiment 2

[0034] Step 1. Strain activation and spore suspension preparation

[0035] Three fermentation strains: Trichoderma viride, Trichoderma harzianum and Trichoderma aculeatus were respectively activated on PDA medium, cultured at 28°C for 72 h, and the conidia were washed with sterilized distilled water to make spore liquid, spore liquid The number of spores is 10 6 A / mL.

[0036] Step 2, preparation of solid seeds

[0037]Mix bran, cassava bagasse, bagasse, and corn flour into a seed fermentation material at a mass ratio of 6.8:1.2:1:1, then mix the seed fermentation material and water at a ratio of 1:1 (mass ratio), and pack them separately into 500mL plastic bottles, the filling volume is 1 / 5 of the height of the plastic bottle, and then sterilized by high-pressure damp heat at 121°C for 20 minutes. Shake evenly, culture at a temperature of 28°C and a humidity of 80% for 5 days; prepare solid seeds of Trichoderma viride, Trichoderma harzianum and Trichoderma aculeatus respec...

Embodiment 3

[0044] Step 1. Strain activation and spore suspension preparation

[0045] Three fermentation strains: Trichoderma viride, Trichoderma harzianum and Trichoderma aculeatus were respectively activated on PDA medium, cultured at 28°C for 72 h, and the conidia were washed with sterilized distilled water to make spore liquid, spore liquid The number of spores is 10 7 A / mL.

[0046] Step 2, preparation of solid seeds

[0047] Mix bran, cassava bagasse, bagasse, and corn flour into a seed fermentation material at a mass ratio of 6.8:1.2:1:1, then mix the seed fermentation material and water at a ratio of 1:1 (mass ratio), and pack them separately into 500mL plastic bottles, the filling volume is 1 / 5 of the height of the plastic bottle, and then sterilized by high-pressure damp heat at 121°C for 20 minutes. Shake evenly, culture at a temperature of 28°C and a humidity of 80% for 5 days; prepare solid seeds of Trichoderma viride, Trichoderma harzianum and Trichoderma aculeatus respe...

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Abstract

The invention relates to compound spore powder prepared through synergistic solid fermentation of mixed strains of trichoderma and application thereof. The compound spore powder prepared through synergistic solid fermentation of the mixed strains of the trichoderma is prepared through the following steps that 1, the strains are activated, and spore suspension is prepared; 2, solid seeds are prepared, wherein green trichoderma solid seeds, trichoderma harzianum solid seeds and trichoderma asperellum solid seeds are prepared respectively, then fermented green trichoderma solid seeds, fermented trichoderma harzianum solid seeds and fermented trichoderma asperellum solid seeds are mixed according to a ratio of 1 to 1 to 1, and finally, mixed strain seeds are obtained; 3, the mixed strain seedsare subjected to solid disc fermentation; 4, the compound spore powder is obtained. According to the compound spore powder prepared through synergistic solid fermentation of the mixed strains of thetrichoderma and the application thereof, a mixed strain seed solid fermentation technology is adopted for obtaining the compound spore powder of three kinds of trichoderma, the compound spore powder can effectively inhibit soil harmful bacteria, improve continuous cropping land and saline and alkaline land, and promote plant growth, and use of chemical fertilizer and pesticides is reduced.

Description

1. Technical field [0001] The invention relates to a microbial bacterial agent for preventing and controlling crop diseases, in particular to a composite spore powder prepared by Trichoderma mixed bacteria cooperative solid fermentation and application thereof. 2. Background technology [0002] The important development trend of the development of biological control products at home and abroad is from single-strain fermentation to mixed-bacteria fermentation, using the functional characteristics of different microorganisms, through the synergy of multiple strains, improving the biomass and functional characteristics of the strains, and simplifying the production process , Reduce fermentation batches, improve fermentation efficiency and shorten fermentation cycle, so that the products developed by it have complementary functions and mechanisms. [0003] Trichoderma belongs to the phylum Deuteromycota, the order Hyphospora, and the genus Trichoderma. It is a kind of beneficia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P3/00A01P21/00C09K17/14C12R1/885
CPCA01N63/30C09K17/14C12N1/14C12N3/00
Inventor 刘铜侯巨梅
Owner HAINAN UNIVERSITY
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