Stable oxygen-carrying microvesicle, and preparation method and application thereof

A microbubble and oxygen-carrying technology, applied in the field of medicine and medicine

Inactive Publication Date: 2019-03-29
彭盛 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The research on the oxygen-carrying microbubbles used for radiotherapy sensitization disclosed by the present invention has no relevant reports at home and abroad. This technology combined with ultrasonic control and microbubble oxygen supply has good application and development prospects, and provides a safe and reliable method for clinical practice. and efficient radiation sensitization scheme

Method used

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  • Stable oxygen-carrying microvesicle, and preparation method and application thereof
  • Stable oxygen-carrying microvesicle, and preparation method and application thereof
  • Stable oxygen-carrying microvesicle, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of micron-sized phospholipid oxygen-carrying microbubbles by freeze-drying method

[0032] Use an analytical balance to measure 20 mg of distearoylphosphatidylcholine (DSPC), 20 mg of dipalmitoylphosphatidylglycerol (DPPG), 2 mg of palmitic acid and 100 mg of polyethylene glycol 4000 (PEG4000) into a flask 5mL of propylene glycol was added to the flask, heated in a water bath at 55°C for 20min, then 35ml of 10wt% aqueous glycerin was added, and heated in a water bath at 65°C for 20min. Heating was stopped, and the phospholipid solution was cooled to room temperature. Then put the phospholipid solution in an ice-water bath, add 3.2ml of perfluoroheptane, and emulsify the above mixture for one minute at 13,000-17,000rpm using a high-speed homogenizer. The average particle size of the obtained perfluoroheptane emulsion is between 1.0 μm and 4 μm. The perfluoroheptane emulsion was allowed to stand until it returned to room temperature and then centr...

Embodiment 2

[0033] Example 2: Preparation of nanoscale phospholipid oxygen-carrying microbubbles by freeze-drying method

[0034] The preparation method of the phospholipid solution is the same as that of Example 1. After adding 3.2ml of perfluoroheptane to the phospholipid solution, the above-mentioned mixed solution is emulsified for one minute using an ultrasonic cell disruptor, and the average particle size of the obtained perfluoroheptane emulsion is between 0.2 μm and 0.8 μm. between μm. Subsequent perfluoroheptane emulsion purification and freeze-drying, and microbubble preparation methods are the same as in Example 1.

Embodiment 3

[0035] Example 3: Preparation of targeted phospholipid oxygen-carrying microbubbles for freeze-drying and long-term storage

[0036] Use an analytical balance to measure 16 mg of distearoylphosphatidylcholine (DSPC), 16 mg of dipalmitoylphosphatidylglycerol (DPPG), 2 mg of palmitic acid, and 4 mg of distearoylphosphatidylethanolamine-polyethylene glycol (DSPE-mPEG2000), 4 mg of distearoylphosphatidylethanolamine-polyethylene glycol-folate (DSPE-PEG-FA) or distearoylphosphatidylethanolamine-polyethylene glycol-cyclopeptide (DSPE-PEG- cRGD) and 100 mg of polyethylene glycol 4000 (PEG4000) into a flask, add 5 mL of propylene glycol to the flask, heat in a water bath at 55 ° C for 20 min, then add 35 ml of 10 wt % glycerol aqueous solution, and heat in a water bath at 65 ° C 20min. Subsequent emulsification with perfluoroheptane, purification and lyophilization of the obtained emulsion, and preparation of microbubbles are the same as in Example 1.

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Abstract

The invention discloses an oxygen-carrying microvesicle, which is easy to use, can high-effectively carry oxygen, has good stability and is uniform in particle size, and a preparation method thereof,as well as application thereof as internal and external irradiation sensitizers during radiotherapy. The microvesicle includes a case and filling gas, wherein the case includes phospholipid and a stabilizer and the filling gas includes oxygen and a fluorine-containing gas.

Description

technical field [0001] The invention relates to the technical field of medicine and medicine, in particular to a stable oxygen-carrying microbubble and its preparation method and application. Background technique [0002] In recent years, the prevalence and mortality of cancer in my country have been increasing year by year, seriously threatening people's health. Conventional tumor treatment methods are mainly surgery, radiotherapy and chemotherapy. Among them, radiotherapy (radiotherapy, radiotherapy) is a kind of local treatment method that uses radiation to treat tumors. Nearly 70% of malignant tumor patients need radiation therapy during treatment, and 40% of malignant tumors can be cured by radiotherapy. The basic principle of radiotherapy is to use α, β, γ rays produced by radioactive isotopes and x-rays, electron beams, proton beams and other particle beams produced by various X-ray therapy machines or accelerators to ionize water molecules to generate free radicals...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/50A61P35/00
CPCA61K9/0019A61K9/5015A61K9/5031A61K41/0038A61P35/00
Inventor 彭盛宋汝渊张振姚书怀
Owner 彭盛
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