Urine exosome extraction reagent tube and manufacturing method thereof

Abstract

The invention discloses a urine exosome extraction reagent tube and a manufacturing method, the urine exosome extraction reagent tube comprises a cap, an outer tube and an inner tube, the top end surface of the outer tube is sleeved with the cap through threads, the inner tube is sleeved with the outer tube, and the inner wall of the inner tube is provided with a nanofiber membrane. The whole reaction can be completed in a short time, and long-time waiting is not needed; by utilizing a positive and negative charge attraction principle, not only is the pollution of a separation reagent avoided, but also non-charged lipoprotein with the particle size of 50-200nm in the urine can be removed; the pore size of the positive charge porous nanofiber membrane is about 50-200nm, so that the high-purity exosome with the particle size of 50-200nm can be obtained, and the pollution of microvesicles with other particle sizes can be prevented; and the porous structure on the positive charge porous nanofiber membrane greatly increases the contact area between the membrane and a sample, and increases the extraction efficiency of the urine exosome.

Description

technical field [0001] The invention relates to the technical field of exosome extraction, in particular to a urine exosome extraction reagent tube and a production method. Background technique [0002] Exosomes were first seen in 1981. EG Trams et al. found small vesicles with a membrane structure in the supernatant of sheep erythrocytes cultured in vitro, and named them exosomes. For the role of exosomes, it was speculated that it was a way for cells to excrete waste. In 1996, GRaposo et al. found that immune cells similar to B lymphocytes also secreted antigen presenting vesicles, and the secreted exosomes could directly stimulate the anti-tumor response of effector CD4+ cells. In 2007, HValadi et al. further discovered that cells can exchange genetic material through RNA in exosomes. With more and more studies on exosomes, researchers have found that they are widely involved in various biological processes such as the body's immune response, antigen presentation, cell ...

AI Technical Summary

Problems solved by technology

[0005] Existing exosome techniques are time-consuming (ranging from 40min to 10 hours), and there are different degrees of contamination of extraction reagents (such as residues of precipitation reagents in the PEG precipitation method, such as residues of high-salt reagents in the column-passing method, such as magnetic In the bead adsorption method, the pollution of the magnetic bead anchor chain antibody, the pollution of the eluent EDTA), although the positive char

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