Method capable of rapidly intuitively distinguishing pine nut extract

An extractive and intuitive technology, applied in the field of rapid and intuitive identification of juniper berry extracts, can solve problems such as troubles in extracts, failure to appear spots, inability to separate extracts, etc., to simplify the processing process, to judge the results clearly, and to save money. Effects of Reagents

Inactive Publication Date: 2019-03-29
JIANGSU ALAND NOURISHMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The developer in the current identification method of juniper berry extract cannot separate the extract, and after spraying the chromogenic agent, no clear spots appear
Caused a lot of confusion on how to identify the extract

Method used

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  • Method capable of rapidly intuitively distinguishing pine nut extract
  • Method capable of rapidly intuitively distinguishing pine nut extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] Reagents and preparation used in this method: guaiazulene (source: Shanghai Yuanye batch number: Y19S6S2975)

[0025] Eucalyptol (source: CIQ Batch No.: 110788-201707)

[0026] n-Hexane (Source: China Inspection Institute Batch No.: 110788-201707)

[0027] Anisaldehyde (Source: Sinopharm Group Batch No.: 20151209)

[0028] Acetic acid (Source: Sinopharm Group Batch No.: 20180806)

[0029] Methanol (Source: Sinopharm Group No.: 20180822)

[0030] Sulfuric Acid (Source: Sinopharm Group Batch Number: 20180507)

[0031] Reference substance solution: accurately weigh 4.0 mg of guaiazulene, add 50 μl of cineole, dissolve and dilute to 10 ml with n-hexane;

[0032] Sample solution: Weigh 5.0g of sample, add 20ml of methanol for ultrasonic extraction, and take the supernatant to obtain;

[0033] Developer solution: ethyl acetate: toluene (19:1, V / V);

[0034] Chromogen solution: anisaldehyde: acetic acid: methanol: sulfuric acid solution = 0.5:10:85:5;

[0035] Detection...

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Abstract

The invention relates to a method capable of rapidly intuitively distinguishing pine nut extract, and discloses an analysis method for thin layer identification of juniper berry extracts. The juniperberry extracts can be distinguished quickly and clearly by using an efficient thin layer chromatograph. A clearly thin-layer chromatogram can be obtained by carrying out distillation, centrifuging, extracting, and the like on the juniper berry extracts, performing processing and extracting on the extracts, and expanding through a developing instrument. The method comprises the following steps of:(1) preparing a test solution; (2) preparing a control substance solution; (3) preparing a sample solution; (4) preparing a developer solution; and sucking 20MuL of a sample solution of the developinginstrument and 10MuL of a reference solution, respectively pointing on the same thin layer silica gel plate G, spraying a developer solution, heating 5-10min at 100-105 DEG C, detecting under sunlight, and spots of the same color are displayed at positions corresponding to the reference color spectrum in a sample chromatograph.

Description

technical field [0001] The invention relates to the technical field of biology and new medicine, in particular to a method capable of quickly and intuitively identifying juniper berry extract. Background technique [0002] Juniper berry is also known as juniper fruit. 1. Antibacterial effect 2. Effect on skin: juniper berry extract is a good helper for oily skin with clogged pores, deep cleansing and purification, effective in treating acne and acne, and is also good against cellulite; astringent, sterilizing and detoxifying , very suitable for treating acne, eczema, dermatitis and psoriasis; 3. Physiological effects: juniper berry extract can detoxify the liver and strengthen liver function; it is also a good household anti-infection agent, which can eliminate congestion and help 4. Effect on nervous system: Juniper berry extract can stimulate tired nerves, remove stress, bring vitality and purify the mind. [0003] The developing agent in the current identification metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/95G01N30/06
CPCG01N30/06G01N30/90G01N30/95
Inventor 奚栋梁毛沁张沛霞徐小明包亚君
Owner JIANGSU ALAND NOURISHMENT
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