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Preparation method and use method of feed for relieving chicken manganese poisoning symptoms

A manganese poisoning and feed technology, applied to bacteria, animal feed, animal feed and other directions used in food preparation, can solve the problems of low absorption and utilization rate, destruction of feed vitamins and other active substances, manganese pollution, etc., so as to alleviate manganese poisoning. Effect

Inactive Publication Date: 2019-04-02
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Traditional manganese preparations, such as manganese sulfate, manganese oxide or manganese chloride, can destroy vitamins and other active substances in the feed, and the absorption and utilization rate is very low. A large amount of unabsorbed manganese is excreted with feces, which wastes limited Manganese resources have brought potential manganese pollution to the environment

Method used

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  • Preparation method and use method of feed for relieving chicken manganese poisoning symptoms

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Effect test

Embodiment 1

[0033] A preparation method of a feed for alleviating symptoms of manganese poisoning in chickens, comprising the following steps:

[0034] 1) Ultrasonic crushing of freshly salvaged cyanobacteria to obtain a cyanobacteria crushing liquid; the ultrasonic crushing time is 15 minutes, and the output power of the ultrasonic crusher used is 1 kilowatt;

[0035] 2) Add a manganese chloride solution with a final concentration of 20 g / L to the crushed cyanobacteria, ferment under anaerobic conditions at a temperature of 37° C., and cultivate for 24 hours to obtain a cyanobacteria fermentation liquid;

[0036] 3) Place the Lactobacillus acidophilus powder in the MRS liquid medium for activation, the mass ratio of the Lactobacillus acidophilus powder to the MRS liquid medium is 1:100, the temperature is 37°C, the rotation speed is 100rpm, and the OD600 value is When it is 0.8, collect the bacterial solution, which is the activation solution;

[0037] 4) Add a final concentration of 10...

Embodiment 2

[0040] A preparation method of a feed for alleviating symptoms of manganese poisoning in chickens, comprising the following steps:

[0041] 1) Ultrasonic crushing of freshly salvaged cyanobacteria to obtain a cyanobacteria crushing liquid; the ultrasonic crushing time is 15 minutes, and the output power of the ultrasonic crusher used is 1 kilowatt;

[0042] 2) Add a manganese chloride solution with a final concentration of 30 g / L to the cyanobacteria broken liquid, ferment under anaerobic conditions at a temperature of 37° C., and cultivate for 24 hours to obtain a cyanobacterial fermentation liquid;

[0043] 3) Place the Lactobacillus acidophilus powder in the MRS liquid medium for activation, the mass ratio of the Lactobacillus acidophilus powder to the MRS liquid medium is 2:100, the temperature is 37°C, the rotation speed is 120rpm, and the OD600 value is When it is 0.9, collect the bacterial solution, which is the activation solution;

[0044] 4) Add a final concentratio...

Embodiment 3

[0047] A preparation method of a feed for alleviating symptoms of manganese poisoning in chickens, comprising the following steps:

[0048] 1) Ultrasonic crushing of freshly salvaged cyanobacteria to obtain a cyanobacteria crushing liquid; the ultrasonic crushing time is 15 minutes, and the output power of the ultrasonic crusher used is 1 kilowatt;

[0049]2) Add a manganese chloride solution with a final concentration of 50 g / L to the cyanobacteria broken liquid, ferment under anaerobic conditions at a temperature of 37° C., and cultivate for 24 hours to obtain a cyanobacterial fermentation liquid;

[0050] 3) Place the Lactobacillus acidophilus powder in the MRS liquid medium for activation, the mass ratio of the Lactobacillus acidophilus powder to the MRS liquid medium is 3:100, the temperature is 37°C, the rotation speed is 150rpm, and the OD600 value is When it is 1.0, the bacterial liquid is collected as the activation liquid;

[0051] 4) Add a final concentration of 10...

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Abstract

The present invention relates to a preparation method and a use method of a feed for relieving chicken manganese poisoning symptoms. The preparation method comprises the following steps: blue-green algae is ultrasonically crushed to obtain blue-green algae crushing liquid; a manganese chloride solution with a final concentration of 50 g / L is added into the blue-green algae crushing liquid and blue-green algae fermentation liquid is obtained under anaerobic conditions; lactobacillus acidophilus powder is placed in an MRS liquid culture medium for activation to obtain activation liquid; blue-green algae fermentation mixed liquid is prepared; the activation liquid inoculates into the blue-green algae fermentation mixed liquid to obtain fermentation liquid; the fermentation liquid is mixed uniformly with corn, millet, sorghum and soybean cake; and a mass ratio of the fermentation liquid, corn, millet, sorghum and soybean cake is 35:30:10:14:11 to obtain the feed. The feed can sufficientlyensure manganese element required for growth of chickens and other domestic poultry animals, can also alleviate the manganese poisoning symptoms, significantly improves growth rate of the chickens andreduces mortality caused by manganese poisoning.

Description

technical field [0001] The patent application for the present invention is a divisional application. The original application number is: 201610004137.3; the original application date is: January 5, 2016; the original application name is: a feed for alleviating the symptoms of manganese poisoning in chickens and its preparation and use method. [0002] The invention relates to the technical field of feed, in particular to a method for preparing and using a feed for alleviating symptoms of manganese poisoning in chickens. Background technique [0003] Manganese is one of the essential trace elements for animals. It has important physiological functions such as promoting growth, enhancing immunity and improving animal reproductive performance. [0004] In the feed of poultry animals such as chickens, the content of manganese should be neither too high nor too low. If manganese is lacking, it is easy to cause abnormal carbohydrate and lipid metabolism, bone deformity, growth s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/75A23K20/20A23K10/12A23K10/30A23K10/37A23K20/26
CPCA23K10/12A23K10/30A23K10/37A23K20/26A23K20/30A23K50/75A23V2400/113Y02P60/87
Inventor 吴迪董娜牛步月
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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