Feed capable of relieving symptom of manganism of chickens, as well as preparation method and using method of feed
A technology of manganese poisoning and feed, which is applied in the field of feed to alleviate the symptoms of chicken poisoning, can solve the problems of low absorption and utilization, destruction of feed vitamins and other active substances, manganese pollution, etc., and achieve the effect of alleviating manganese poisoning
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Embodiment 1
[0032] A preparation method of a feed for alleviating symptoms of manganese poisoning in chickens, comprising the following steps:
[0033] 1) Ultrasonic crushing of freshly salvaged cyanobacteria to obtain a cyanobacteria crushing liquid; the ultrasonic crushing time is 15 minutes, and the output power of the ultrasonic crusher used is 1 kilowatt;
[0034] 2) Add manganese chloride solution to the cyanobacteria broken liquid, the final concentration of manganese chloride is 20g / L, ferment under anaerobic conditions at a temperature of 37°C, and cultivate for 24 hours to obtain a cyanobacteria fermentation liquid;
[0035] 3) Place the Lactobacillus acidophilus powder in the MRS liquid medium for activation, the mass ratio of the Lactobacillus acidophilus powder to the MRS liquid medium is 1:100, the temperature is 37°C, the rotation speed is 100rpm, and the OD600 value is When it is 0.8, collect the bacterial solution, which is the activation solution;
[0036] 4) Add dipota...
Embodiment 2
[0039] A preparation method of a feed for alleviating symptoms of manganese poisoning in chickens, comprising the following steps:
[0040] 1) Ultrasonic crushing of freshly salvaged cyanobacteria to obtain a cyanobacteria crushing liquid; the ultrasonic crushing time is 15 minutes, and the output power of the ultrasonic crusher used is 1 kilowatt;
[0041] 2) Add manganese chloride solution to the cyanobacteria broken liquid, the final concentration of manganese chloride is 30g / L, ferment under anaerobic conditions at a temperature of 37°C, and cultivate for 24 hours to obtain a cyanobacteria fermentation liquid;
[0042] 3) Place the Lactobacillus acidophilus powder in the MRS liquid medium for activation, the mass ratio of the Lactobacillus acidophilus powder to the MRS liquid medium is 2:100, the temperature is 37°C, the rotation speed is 120rpm, and the OD600 value is When it is 0.9, collect the bacterial solution, which is the activation solution;
[0043] 4) Adding dip...
Embodiment 3
[0046] A preparation method of a feed for alleviating symptoms of manganese poisoning in chickens, comprising the following steps:
[0047] 1) Ultrasonic crushing of freshly salvaged cyanobacteria to obtain a cyanobacteria crushing liquid; the ultrasonic crushing time is 15 minutes, and the output power of the ultrasonic crusher used is 1 kilowatt;
[0048] 2) Add manganese chloride solution to the cyanobacteria crushed liquid, the final concentration of manganese chloride is 50g / L, ferment under anaerobic conditions at a temperature of 37°C, and cultivate for 24 hours to obtain a cyanobacteria fermentation liquid;
[0049]3) Place the Lactobacillus acidophilus powder in the MRS liquid medium for activation, the mass ratio of the Lactobacillus acidophilus powder to the MRS liquid medium is 3:100, the temperature is 37°C, the rotation speed is 150rpm, and the OD600 value is When it is 1.0, the bacteria liquid is collected, which is the activation liquid;
[0050] 4) Adding dip...
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