A Simultaneous and Accurate Detection Method of 45 Kinds of Antibiotics in Livestock Feces

Abstract

The invention discloses a method for simultaneously and accurately detecting 45 kinds of antibiotics in livestock and poultry feces, comprising: establishment of antibiotic standard curves, sample detection and the like. The new method obtains the components and proportions of the extract through screening, so that antibiotics with different properties can be extracted at the same time, realizing the accurate detection of more antibiotics at the same time. During the detection, the overall RSD of the 45 antibiotics was reduced, the detection error was significantly reduced, and the detection accuracy was improved. In the new method, the separation and detection of 45 kinds of antibiotics in the five major classes of antibiotics was realized within 12 minutes, which has the characteristics of strong operability, high efficiency, rapidity and high accuracy.

Description

technical field [0001] The invention belongs to the technical field of solid waste recycling, and relates to a method for simultaneously and accurately detecting 45 kinds of antibiotics in livestock and poultry manure. Background technique [0002] my country is a big breeding country and a big consumer of antibiotics. According to reports, in 2013, the total antibiotic consumption in my country was about 162,000 tons (about 9 times the total usage in the United States), of which 52% was used in livestock and poultry farming. It can be seen that the consumption of veterinary antibiotics in my country has occupied a pivotal position. After veterinary antibiotics are used, 30% to 90% are excreted with livestock and poultry feces, and most of them exist in the maternal form. In my country, the main utilization method of livestock and poultry manure is farmland utilization. Therefore, antibiotic residues in livestock and poultry manure will cause secondary pollution to soil, wa...

AI Technical Summary

Problems solved by technology

However, with more and more types of veterinary antibiotics and different habits of farmers choosing antibiotics, there are more and more types of antibiotic residues in livestock and poultry manure, and the physical and chemical properties of different types of antibiotics are quite different. Simultaneous accurate detection of multiple antibiotics poses challenges

Therefore, it is urgent to establish a precise detection method for a wider variety of antibiotics. At present, no simultaneous accurate detection method for 45 kinds (5 major categories) of veterinary antibiotics has been reported.

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